Journal: Molecules

Article Title: Efficiency of the Enzymatic Conversion of Flavone Glycosides Isolated from Carrot Leaves and Anti-Inflammatory Effects of Enzyme-Treated Carrot Leaves

doi: 10.3390/molecules28114291

Figure Lengend Snippet: Effect of DCR, DCL, and enzyme-treated DCL on the transcript levels of TNF-α and cytokines under various stimulatory conditions. ( A , B ) The mRNA levels of TNF-α and IL-2 were assayed using RT-PCR. Jurkat cells were stimulated using PMA (100 ng/mL)/PHA (10 ng/mL) and treated with various extracts, as indicated for 3 h. The mRNA levels of TNF-α and IL-2 were determined using qRT-PCR and normalized to those of GAPDH. ( C , D ) TNF-α and IL-2 levels were measured using an enzyme-linked immunosorbent assay (ELISA). Cells were stimulated with PMA (100 ng/mL)/PHA (10 ng/mL) and cotreated with various extracts, as indicated for 24 h. After simulation, the supernatants were collected for ELISA. The values are presented as the mean ± SD for three independent experiments. ### p < 0.0001 vs. control and **** p < 0.0001, *** p < 0.001, ** p < 0.005, * p < 0.05 vs. PMA/PHA.

Article Snippet: TNF-α and IL-2 levels in the cell supernatants were measured using a Human TNF-α and IL-2 ELISA kit (Abcam, Cambridge, England), according to the manufacturer’s instructions.

Techniques: Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Physiology

Article Title: Impact of Bilateral Sympathetic Stellate Ganglionectomy on TGF-β1 Signaling Pathway in Rats With Chronic Volume Overload

doi: 10.3389/fphys.2020.00375

Figure Lengend Snippet: Expressions of norepinephrine (NE) and acetylcholine (ACh) in the plasma, right ventricle (RV), and left ventricle (LV) by ELISA. (A) The expression of NE was significantly increased in the abdominal aorta-cava fistula (AV) group and decreased in the bilateral sympathetic stellate ganglionectomy after abdominal aorta-cava fistula (AD) group in the plasma, RV, and LV. (B) The expression of ACh was significantly decreased in the AV group and increased in the AD group in the RV (* P < 0.05, ** P < 0.01) (three replicate experiments were performed).

Article Snippet: Acetylcholine (ACh) concentrations of the plasma, RV, and LV were determined with the ELISA kit (BioVision, E4454-100, Milpitas, CA, United States) following the manufacturer’s instructions.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing