Journal: Stem Cell Research & Therapy

Article Title: Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro

doi: 10.1186/s13287-020-01671-1

Figure Lengend Snippet: Results of RT-PCR production of spermatogonial stem cells and EL4 cells. The expression of PLZF, GFRα-1, and Integα-6 in spermatogonial stem cells and H2Kb for EL4 cells. β-actin was included as a housekeeping gene

Article Snippet: Finally, in the present study, three main groups were designed, and by accessing the culture collection of Pasteur Institute, Tehran, Iran, the mouse acute lymphoblastic leukemia cell line EL4 was prepared.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro

doi: 10.1186/s13287-020-01671-1

Figure Lengend Snippet: Results of flow cytometry in the mixture of cancer cells and spermatogonial stem cells after microfluidic isolation. a The results obtained from cells without adding antibodies. b The results of EL4 cell composition and SSCs (in vitro tumor model). c The results of cell percentage after passing microfluidic of outer outlet. d The results of the percentage of cells associated with the inner outlet. Q1, the range of PLZF positive cells and negative H2k cells; Q2, the range of PLZF positive cells and positive H2k cells, Q3, the range of negative PLZF cells and negative H2k cells; Q4, the range for negative PLZF and positive H2k

Article Snippet: Finally, in the present study, three main groups were designed, and by accessing the culture collection of Pasteur Institute, Tehran, Iran, the mouse acute lymphoblastic leukemia cell line EL4 was prepared.

Techniques: Flow Cytometry, Isolation, In Vitro

Journal: Stem Cell Research & Therapy

Article Title: Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro

doi: 10.1186/s13287-020-01671-1

Figure Lengend Snippet: SSC colonies of mouse neonate spermatogonial stem cell after 2 weeks of culture in free-growth factors DMEM/F12, 1 week after primary culture, and EL4 tumor cell. a Complete colony of spermatogonium cells. b Tumor cells. Scale bars = 100 μm

Article Snippet: Finally, in the present study, three main groups were designed, and by accessing the culture collection of Pasteur Institute, Tehran, Iran, the mouse acute lymphoblastic leukemia cell line EL4 was prepared.

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro

doi: 10.1186/s13287-020-01671-1

Figure Lengend Snippet: Part A is for the PLZF marker for SSC cells and part B is for tumor cell CD45 markers and the MERGE-shaped image of the above images and DAPI staining. Based on flow cytometry, in the first row, the control group, which contains the SSC and EL4 cell composition, is seen, and both types of cells are seen. In the 2nd row is the output from the outer inlet, which indicates a higher number of EL4 cell. The 3rd row refers to the output of the inner outlet which indicates a higher number of PLZF markers, the SSC cell

Article Snippet: Finally, in the present study, three main groups were designed, and by accessing the culture collection of Pasteur Institute, Tehran, Iran, the mouse acute lymphoblastic leukemia cell line EL4 was prepared.

Techniques: Marker, Staining, Flow Cytometry, Control

Journal: Nmr in Biomedicine

Article Title: Analysis of 13 C and 14 C labeling in pyruvate and lactate in tumor and blood of lymphoma‐bearing mice injected with 13 C‐ and 14 C‐labeled pyruvate

doi: 10.1002/nbm.3901

Figure Lengend Snippet: Biodistribution [percentage of injected dose (ID%)/g] of [1‐ 14 C] pyruvate injected intravenously (10 mL/kg; 82mM; specific activity, 50–60 mCi/mmol). EL4 tumor‐bearing mice were injected at the indicated times and the tissues were harvested and weighed, and the radioactivity was counted in a scintillation counter. The total amounts of radioactivity recovered from the indicated tissues were 55.9, 57.9 and 56.1 ID%/g at 15, 30 and 60 s, respectively

Article Snippet: Tumors were established by subcutaneous inoculation of a suspension of 5 × 10 6 viable EL4 murine lymphoma cells (in a volume of 100 μL) in the left flank of C57BL/6 mice (Charles River Ltd., Harlow, Essex, UK) ( n = 69) at 6–8 weeks of age, when the animals were ~20 g. Studies were performed when the tumors had grown to a size of ~2 cm 3 , which was typically 10 days following implantation.

Techniques: Injection, Activity Assay, Radioactivity

Journal: Scientific Reports

Article Title: In vivo imaging of T cell lymphoma infiltration process at the colon

doi: 10.1038/s41598-018-22399-2

Figure Lengend Snippet: Localization of EL4-EGFP cells in the blood vessels adjacent to crypts in the colon of C57BL6/J mice. ( A ) EL4 cells stably expressing EGFP in vitro under fluorescence microscopy (LSM710, Carl Zeiss). Bar indicates 50 μm. ( B ) EGFP fluorescence intensity measured using a cell analyzer (SH800, Sony). ( C ) At 1 to 3 weeks after EL4-EGFP cell injection, the colon was removed from the body and observed on living tissues. ( D ) EL4-EGFP cell imaging of in the colon. Green color indicates EGFP of EL4-EGFP cells, as shown with white arrows. Blood vessels were stained with rhodamine B–conjugated dextran (M.W. 70,000) (red). Bar indicates 50 μm. Right upper panel shows an enlarged image of an elongated EL4 cell. Bar indicates 10 μm. Right lower panel shows an EL4 cell lodged in the T-junction of blood vessels. Bar indicates 10 μm. These images were representative from 3 mice examined. ( E ). Imaging of EL4-EGFP cells localized in large blood vessels under the crypts in the mucosal layer. EL4-EGFP cells are indicated by white arrows. Bar indicates 50 μm. ( F ). Crypts visualized using green mice (C57BL/6-Tg[CAG-EGFP]). Crypts and blood vessels are shown in green and red, respectively. Bar indicates 50 μm.

Article Snippet: EL4 mouse lymphoma cells were purchased from Japanese Collection of Research Bioresources.

Techniques: Stable Transfection, Expressing, In Vitro, Fluorescence, Microscopy, Injection, Imaging, Staining

Journal: Scientific Reports

Article Title: In vivo imaging of T cell lymphoma infiltration process at the colon

doi: 10.1038/s41598-018-22399-2

Figure Lengend Snippet: Localization of EL4-DsRed2 cells in blood vessels adjacent to crypts in the colon of green mice. ( A ) EL4 cells stably expressing DsRed2 in vitro observed under a fluorescence microscope. Bar indicates 50 μm. (pCAG-DsRed2 sequence is in supplementary data2). ( B ) DsRed2 fluorescence intensity measured using a cell analyzer (SH800, Sony). ( C ) Imaging of EL4-DsRed2 cells in the colon. Red color indicates DsRed2 in EL4-DsRed2 cells, as shown by white arrowheads. Green color mainly shows endothelial cells of blood vessels. Bar indicates 50 μm. This image was representative from 4 mice examined. ( D ) Analyses of the diameter of EL4-EGFP cells in vitro and in colonic blood vessels. Error values and bars indicate standard error (S.E.). ( E ) Hematoxylin and eosin staining and fluorescence analysis of the infiltration of EL4 cells into colon tissues. EL4 cells (5 × 10 5 ) were injected into 9-week-old C57BL6/J mice via the tail vein. The colon was removed and fixed with 4% paraformaldehyde at 14 days after EL4 cell injection. The colon tissue was stained with hematoxylin and eosin. Bar indicates 50 μm. ( F ) Infiltration of EL4 cells was also examined by fluorescence microscopy with the same sample used in ( E ).

Article Snippet: EL4 mouse lymphoma cells were purchased from Japanese Collection of Research Bioresources.

Techniques: Stable Transfection, Expressing, In Vitro, Fluorescence, Microscopy, Sequencing, Imaging, Staining, Injection

Journal: Scientific Reports

Article Title: In vivo imaging of T cell lymphoma infiltration process at the colon

doi: 10.1038/s41598-018-22399-2

Figure Lengend Snippet: Time-lapse imaging of EL4-EGFP cells in colonic blood vessels. ( A ) Time-lapse (20-min intervals) imaging of EL4-EGFP cells (green) in small blood vessels (red). EL4-EGFP cells (5 × 10 5 ) were injected into the tail vein of C57BL6/J mice and observed 10 days after injection. White arrows show lodged EL4-EGFP cells. Blue arrows indicate moving EL4 cells. Bar indicates 100 μm. ( B ) Tracking analysis of EL4-EGFP cell movement for 1 h. Traces of moving cells are shown in red. Bar indicates 100 μm. ( C ). Ratio of moving and lodged cells determined at 20-min intervals. A total of 23.3 ± 0.32% of the EL4 cells exhibited motility. Analyses were performed from 3 mice studied in the early stage of metastasis.

Article Snippet: EL4 mouse lymphoma cells were purchased from Japanese Collection of Research Bioresources.

Techniques: Imaging, Injection

Journal: Scientific Reports

Article Title: In vivo imaging of T cell lymphoma infiltration process at the colon

doi: 10.1038/s41598-018-22399-2

Figure Lengend Snippet: Time-lapse imaging of highly motile EL4-EGFP cells in colonic blood vessels. ( A ) Dynamics of EL4 cells examined at subcellular resolution. Migration of two EL4 cells is shown with blue and orange lines. Bar indicates 50 μm. ( B ) Analysis of distance traversed by EL4 cells each min. ( C ). Observation of two individual EL4 cells in small vessel in the colon. Note that we observed a pseudopod (white arrow) extending from one EL4 cell (blue arrow) and touching the other cell (orange arrow). Bar indicates 50 μm.

Article Snippet: EL4 mouse lymphoma cells were purchased from Japanese Collection of Research Bioresources.

Techniques: Imaging, Migration

Journal: Scientific Reports

Article Title: In vivo imaging of T cell lymphoma infiltration process at the colon

doi: 10.1038/s41598-018-22399-2

Figure Lengend Snippet: Formation of small nodules composed of several EL4-EGFP cells in crypts and blood vessels. ( A ) 3D image of a small nodule composed of EGFP-EL4 cells that infiltrated into crypts, as shown by a white arrow. Bar indicates 20 μm. ( B ) Image of a small EGFP-EL4 cell nodule in a blood vessel. Bar indicates 20 μm. This image is representative from 3 mice examined. ( C ). Percentage of EL4-EGFP nodules in crypts and blood vessels. ( D ). Schematic representation of the formation of nodules composed of EL4-EGFP cells. Green and red color indicate EL4-EGFP cells and blood vessels, respectively.

Article Snippet: EL4 mouse lymphoma cells were purchased from Japanese Collection of Research Bioresources.

Techniques: