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mmp13  (Boster Bio)
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Boster Bio mmp13
Inflammation inhibition and chondrocyte protection by UCMSCs. ( A ) Detection of inflammatory factors in synovial fluid. ELISAs were performed to detect IL-1β, TNF-α, and <t>MMP13</t> in synovial fluid. The levels of these three factors decreased significantly in the UCMSC group compared with the model group, indicating that UCMSC transplantation inhibited the expression of key inflammatory factors in OA. Normal, normal group; Model, OA model group; Vehicle, OA model with saline injection; UCMSC, OA model with UCMSC transplantation. n = 6. ( B ) The effect of UCMSCs on the gene expression levels of aggrecan , Col2, and Sox9 in chondrocytes induced by IL-β1. Chondrocytes co-cultured with UCMSCs and IL-β1 were collected, and RT-PCR was used to detect gene expression levels in chondrocytes. Treatment of chondrocytes with high (1 × 10 6 cells) and low (0.5 × 10 6 cells) doses of UCMSCs promoted the expression of aggrecan , Col2 , and Sox9 genes. Control, normal cultured rat chondrocytes; Model, rat chondrocytes pre-treated by 10 ng/mL IL-1β; UCMSC-L, rat chondrocytes pre-treated by 10 ng/mL IL-1β were co-cultured with 0.5 × 10 6 UCMSCs; UCMSC-H, rat chondrocytes pre-treated by10ng/mL IL-1β were co-cultured with 1 × 10 6 UCMSCs, n = 3. * P < 0.05, ** P < 0.01 analyzed by unpaired two-tailed t-test after normality and equal variance test using Shapiro–Wilk test and ANOVA plus Brown–Forsythe test in the GraphPad software.
Mmp13, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ek0468/Human+MMP13%2FCollagenase+3+ELISA+Kit+PicoKine/pmc10495383-179-16-18
Average 92 stars, based on 1 article reviews
mmp13 - by Bioz Stars, 2026-09
92/100 stars
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Inflammation inhibition and chondrocyte protection by UCMSCs. ( A ) Detection of inflammatory factors in synovial fluid. ELISAs were performed to detect IL-1β, TNF-α, and MMP13 in synovial fluid. The levels of these three factors decreased significantly in the UCMSC group compared with the model group, indicating that UCMSC transplantation inhibited the expression of key inflammatory factors in OA. Normal, normal group; Model, OA model group; Vehicle, OA model with saline injection; UCMSC, OA model with UCMSC transplantation. n = 6. ( B ) The effect of UCMSCs on the gene expression levels of aggrecan , Col2, and Sox9 in chondrocytes induced by IL-β1. Chondrocytes co-cultured with UCMSCs and IL-β1 were collected, and RT-PCR was used to detect gene expression levels in chondrocytes. Treatment of chondrocytes with high (1 × 10 6 cells) and low (0.5 × 10 6 cells) doses of UCMSCs promoted the expression of aggrecan , Col2 , and Sox9 genes. Control, normal cultured rat chondrocytes; Model, rat chondrocytes pre-treated by 10 ng/mL IL-1β; UCMSC-L, rat chondrocytes pre-treated by 10 ng/mL IL-1β were co-cultured with 0.5 × 10 6 UCMSCs; UCMSC-H, rat chondrocytes pre-treated by10ng/mL IL-1β were co-cultured with 1 × 10 6 UCMSCs, n = 3. * P < 0.05, ** P < 0.01 analyzed by unpaired two-tailed t-test after normality and equal variance test using Shapiro–Wilk test and ANOVA plus Brown–Forsythe test in the GraphPad software.

Journal: Scientific Reports

Article Title: Umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosis

doi: 10.1038/s41598-023-42349-x

Figure Lengend Snippet: Inflammation inhibition and chondrocyte protection by UCMSCs. ( A ) Detection of inflammatory factors in synovial fluid. ELISAs were performed to detect IL-1β, TNF-α, and MMP13 in synovial fluid. The levels of these three factors decreased significantly in the UCMSC group compared with the model group, indicating that UCMSC transplantation inhibited the expression of key inflammatory factors in OA. Normal, normal group; Model, OA model group; Vehicle, OA model with saline injection; UCMSC, OA model with UCMSC transplantation. n = 6. ( B ) The effect of UCMSCs on the gene expression levels of aggrecan , Col2, and Sox9 in chondrocytes induced by IL-β1. Chondrocytes co-cultured with UCMSCs and IL-β1 were collected, and RT-PCR was used to detect gene expression levels in chondrocytes. Treatment of chondrocytes with high (1 × 10 6 cells) and low (0.5 × 10 6 cells) doses of UCMSCs promoted the expression of aggrecan , Col2 , and Sox9 genes. Control, normal cultured rat chondrocytes; Model, rat chondrocytes pre-treated by 10 ng/mL IL-1β; UCMSC-L, rat chondrocytes pre-treated by 10 ng/mL IL-1β were co-cultured with 0.5 × 10 6 UCMSCs; UCMSC-H, rat chondrocytes pre-treated by10ng/mL IL-1β were co-cultured with 1 × 10 6 UCMSCs, n = 3. * P < 0.05, ** P < 0.01 analyzed by unpaired two-tailed t-test after normality and equal variance test using Shapiro–Wilk test and ANOVA plus Brown–Forsythe test in the GraphPad software.

Article Snippet: ELISA kits were used to determine the levels of IL-1β (EK0392, Boster, China), TNF-α (EK0525, Boster), MMP13 (EK0468, Boster), IL-10 (EK0416, Boster), and TGF-β1 (EK0513, Boster) in the supernatant.

Techniques: Inhibition, Transplantation Assay, Expressing, Saline, Injection, Gene Expression, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Control, Two Tailed Test, Software