Journal: Nature Communications

Article Title: In-situ cross-linking mass spectrometry reveals compartment-specific proteasomal interactions and structural heterogeneity

doi: 10.1038/s41467-025-65752-6

Figure Lengend Snippet: A Complex model of Rpt5 ( P17980 , residues 166–439) and PSMD9 ( O00233 ), with full torsional freedom allowed for the linker residues (115–134) between PSMD9’s two domains. B AlphaFold model of free PSMD9, with the backbone colored according to pLDDT values (yellow, <70; orange, <50). The arrow indicates the direction of domain closure upon binding to Rpt5. C AlphaFold-modeled complex structure between Rpn5 ( O00232 ), EIF3M ( Q7L2H7 ), and Rpn8 ( P51665 ), shown in two different perspectives. The sequence and structure comparison between EIF3M and Rpn9 ( Q9UNM6 ) are shown in Supplementary Fig. . Dashed lines indicate experimental intermolecular cross-links, with associated Lys residues denoted.

Article Snippet: The following antibodies were used: EIF3M (Proteintech, 11423-1-AP), RPS5 (Santa Cruz, sc-390935), and a control IgG antibody (Proteintech, 30000-0-AP, 1:200 dilution).

Techniques: Binding Assay, Sequencing, Comparison

Journal: Nature Communications

Article Title: In-situ cross-linking mass spectrometry reveals compartment-specific proteasomal interactions and structural heterogeneity

doi: 10.1038/s41467-025-65752-6

Figure Lengend Snippet: A Co-immunoprecipitation confirms that EIF3M interacts with Rpn11, a proteasomal subunit, and with Rps5, a component of the 48S translation initiation complex. Three biological repeats were performed, with the representative micrograph shown here (raw micrographs provided in the Source data). B Immunofluorescence microscopy reveals co-localization of EIF3M with both Rps5 and Rpn11in the cytoplasm and nucleus. Fluorescence intensity measurements indicate that EIF3M/Rpn11 levels are approximately 3–5 times higher in the cytoplasm compared to the nucleus.

Article Snippet: The following antibodies were used: EIF3M (Proteintech, 11423-1-AP), RPS5 (Santa Cruz, sc-390935), and a control IgG antibody (Proteintech, 30000-0-AP, 1:200 dilution).

Techniques: Immunoprecipitation, Immunofluorescence, Microscopy, Fluorescence

Journal: Oncology Letters

Article Title: High expression of eukaryotic initiation factor 3M predicts poor prognosis in colon adenocarcinoma patients

doi: 10.3892/ol.2019.11164

Figure Lengend Snippet: Expression of EIF3M in patients with colon adenocarcinoma. (A) EIF3M protein was observed in the cytoplasm of colon adenocarcinomas (magnification, ×400); (B) EIF3M protein was observed in the cytoplasm of normal colon tissues (magnification, ×400); (C) IRS values for EIF3M expression in colon adenocarcinoma and normal colon tissues; (D) western blot analysis of EIF3M in freeze-thawed tissues; (E) relative protein expression of EIF3M in freeze-thawed tissues; (F) RT-qPCR analysis of EIF3M in freeze-thawed tissues; (G) ELISA analysis of EIF3M in the serum of patients with colon adenocarcinoma and healthy controls. ***P<0.01. EIF3M, eukaryotic initiation factor 3 subunit M; IRS, immunoreactivity score; T, colon tumors; N, normal colon tissues.

Article Snippet: The EIF3M ELISA kit (cat. no. S00143, Shanghai Yuanye Biotechnology Co., Ltd) was used to detect the levels of EIF3M in serum of 20 patients with colon adenocarcinoma patients and 80 healthy controls.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Oncology Letters

Article Title: High expression of eukaryotic initiation factor 3M predicts poor prognosis in colon adenocarcinoma patients

doi: 10.3892/ol.2019.11164

Figure Lengend Snippet: Association of EIF3M expression in patients with colon adenocarcinoma with clinicopathological variables.

Article Snippet: The EIF3M ELISA kit (cat. no. S00143, Shanghai Yuanye Biotechnology Co., Ltd) was used to detect the levels of EIF3M in serum of 20 patients with colon adenocarcinoma patients and 80 healthy controls.

Techniques: Expressing

Journal: Oncology Letters

Article Title: High expression of eukaryotic initiation factor 3M predicts poor prognosis in colon adenocarcinoma patients

doi: 10.3892/ol.2019.11164

Figure Lengend Snippet: Kaplan-Meier survival curves of patients with colon adenocarcinoma divided by EIF3M expression. (A) Overall survival; (B) disease-free survival. EIF3M, eukaryotic initiation factor 3 subunit M.

Article Snippet: The EIF3M ELISA kit (cat. no. S00143, Shanghai Yuanye Biotechnology Co., Ltd) was used to detect the levels of EIF3M in serum of 20 patients with colon adenocarcinoma patients and 80 healthy controls.

Techniques: Expressing

Journal: Oncology Letters

Article Title: High expression of eukaryotic initiation factor 3M predicts poor prognosis in colon adenocarcinoma patients

doi: 10.3892/ol.2019.11164

Figure Lengend Snippet: Prognostic role of EIF3M expression and clinicopathological factors for OS and DFS of patients with colon cancer by univariate Cox regression analysis.

Article Snippet: The EIF3M ELISA kit (cat. no. S00143, Shanghai Yuanye Biotechnology Co., Ltd) was used to detect the levels of EIF3M in serum of 20 patients with colon adenocarcinoma patients and 80 healthy controls.

Techniques: Expressing

Journal: Oncology Letters

Article Title: High expression of eukaryotic initiation factor 3M predicts poor prognosis in colon adenocarcinoma patients

doi: 10.3892/ol.2019.11164

Figure Lengend Snippet: Prognostic role of EIF3M expression and clinicopathological factors for OS and DFS of patients with colon cancer by multivariate Cox regression analysis.

Article Snippet: The EIF3M ELISA kit (cat. no. S00143, Shanghai Yuanye Biotechnology Co., Ltd) was used to detect the levels of EIF3M in serum of 20 patients with colon adenocarcinoma patients and 80 healthy controls.

Techniques: Expressing

Journal: Oncology Letters

Article Title: High expression of eukaryotic initiation factor 3M predicts poor prognosis in colon adenocarcinoma patients

doi: 10.3892/ol.2019.11164

Figure Lengend Snippet: Analysis of EIF3M expression in patients with colon/colorectal cancer from published datasets in the Oncomine database. (A) Six datasets; (B) Co-expression analysis of the Ki Colon dataset. EIF3M, eukaryotic initiation factor 3 subunit M.

Article Snippet: The EIF3M ELISA kit (cat. no. S00143, Shanghai Yuanye Biotechnology Co., Ltd) was used to detect the levels of EIF3M in serum of 20 patients with colon adenocarcinoma patients and 80 healthy controls.

Techniques: Expressing

Journal: Oncology Letters

Article Title: High expression of eukaryotic initiation factor 3M predicts poor prognosis in colon adenocarcinoma patients

doi: 10.3892/ol.2019.11164

Figure Lengend Snippet: Information and functions of the top 15 genes associated with EIF3M expression using the Ki Colon dataset.

Article Snippet: The EIF3M ELISA kit (cat. no. S00143, Shanghai Yuanye Biotechnology Co., Ltd) was used to detect the levels of EIF3M in serum of 20 patients with colon adenocarcinoma patients and 80 healthy controls.

Techniques: Expressing