Journal: Journal of Translational Medicine

Article Title: Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

doi: 10.1186/1479-5876-12-131

Figure Lengend Snippet: Clinical characteristics of patients with EGFR mutated lung adenocarcinoma

Article Snippet: Allele specific assays were run in a final volume of 5 μl in 384 wells plate including 2.5 μl of 2X TaqMan® genotyping master mix (Applied Biosystems), 0.5 μl of 10X Assay Mix (Hs00000173_rf :EGFR_rf; Hs00000141_mu :EGFR_6213_mu; Hs00000104_mu: EGFR_ 6239_mu; Hs00000148_mu: EGFR_6253_mu; Hs00000146_mu: EGFR_6252_mu; Hs00000106_mu: EGFR_6240_mu; Hs00000102_mu: EGFR_6224_mu; Hs00000228_mu: EGFRex19dels_mu from Lifetechnologies) and 1μl DNA template (≤20ng/μl).

Techniques: Biomarker Discovery

Journal: Journal of Translational Medicine

Article Title: Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

doi: 10.1186/1479-5876-12-131

Figure Lengend Snippet: Multi - localization EGFR genotyping screening

Article Snippet: Allele specific assays were run in a final volume of 5 μl in 384 wells plate including 2.5 μl of 2X TaqMan® genotyping master mix (Applied Biosystems), 0.5 μl of 10X Assay Mix (Hs00000173_rf :EGFR_rf; Hs00000141_mu :EGFR_6213_mu; Hs00000104_mu: EGFR_ 6239_mu; Hs00000148_mu: EGFR_6253_mu; Hs00000146_mu: EGFR_6252_mu; Hs00000106_mu: EGFR_6240_mu; Hs00000102_mu: EGFR_6224_mu; Hs00000228_mu: EGFRex19dels_mu from Lifetechnologies) and 1μl DNA template (≤20ng/μl).

Techniques: Mutagenesis

Journal: Journal of Translational Medicine

Article Title: Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

doi: 10.1186/1479-5876-12-131

Figure Lengend Snippet: Multi - localization EGFR CNV screening

Article Snippet: Allele specific assays were run in a final volume of 5 μl in 384 wells plate including 2.5 μl of 2X TaqMan® genotyping master mix (Applied Biosystems), 0.5 μl of 10X Assay Mix (Hs00000173_rf :EGFR_rf; Hs00000141_mu :EGFR_6213_mu; Hs00000104_mu: EGFR_ 6239_mu; Hs00000148_mu: EGFR_6253_mu; Hs00000146_mu: EGFR_6252_mu; Hs00000106_mu: EGFR_6240_mu; Hs00000102_mu: EGFR_6224_mu; Hs00000228_mu: EGFRex19dels_mu from Lifetechnologies) and 1μl DNA template (≤20ng/μl).

Techniques: Mutagenesis

Journal: Journal of Translational Medicine

Article Title: Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

doi: 10.1186/1479-5876-12-131

Figure Lengend Snippet: Illustration of the different patterns of EGFR DEL19 mutation , EGFR CN analyses and the associated histological features for patient P1. - 1 - HES staining at x100 magnification, P1A : lepidic pattern, P1B: solid pattern. - 2 - Fragment analysis for EGFR DEL19 mutation. - 3 - EGFR copy number evaluation. In sample A with lepidic pattern, mutation was validated by a cast PCR assay and there is no increased copy number of EGFR gene (CNV:1.89). In sample B with solid pattern, EGFR amplification was identified (CNV: 3.44).

Article Snippet: Allele specific assays were run in a final volume of 5 μl in 384 wells plate including 2.5 μl of 2X TaqMan® genotyping master mix (Applied Biosystems), 0.5 μl of 10X Assay Mix (Hs00000173_rf :EGFR_rf; Hs00000141_mu :EGFR_6213_mu; Hs00000104_mu: EGFR_ 6239_mu; Hs00000148_mu: EGFR_6253_mu; Hs00000146_mu: EGFR_6252_mu; Hs00000106_mu: EGFR_6240_mu; Hs00000102_mu: EGFR_6224_mu; Hs00000228_mu: EGFRex19dels_mu from Lifetechnologies) and 1μl DNA template (≤20ng/μl).

Techniques: Mutagenesis, Staining, Amplification

Journal: Journal of Translational Medicine

Article Title: Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

doi: 10.1186/1479-5876-12-131

Figure Lengend Snippet: Summary of previously published series

Article Snippet: Allele specific assays were run in a final volume of 5 μl in 384 wells plate including 2.5 μl of 2X TaqMan® genotyping master mix (Applied Biosystems), 0.5 μl of 10X Assay Mix (Hs00000173_rf :EGFR_rf; Hs00000141_mu :EGFR_6213_mu; Hs00000104_mu: EGFR_ 6239_mu; Hs00000148_mu: EGFR_6253_mu; Hs00000146_mu: EGFR_6252_mu; Hs00000106_mu: EGFR_6240_mu; Hs00000102_mu: EGFR_6224_mu; Hs00000228_mu: EGFRex19dels_mu from Lifetechnologies) and 1μl DNA template (≤20ng/μl).

Techniques: Mutagenesis, Sequencing, Laser Capture Microdissection, Adjuvant

Journal: Scientific Reports

Article Title: Design, in silico studies and biological evaluation of novel chalcones tethered triazolo[3,4- a ]isoquinoline as EGFR inhibitors targeting resistance in non-small cell lung cancer

doi: 10.1038/s41598-024-76459-x

Figure Lengend Snippet: Inhibitory activities of chalcones 3e and 3f against EGFR-TKs compared to Lapatinib, Gefitinib, and Erlotinib as reference standards.

Article Snippet: The EGFR proteins (EGFR WT , EGFR T790M and EGFR L858R ) were obtained from Signalchem (BC V6V 2J2, Canada).

Techniques:

Journal: Scientific Reports

Article Title: Design, in silico studies and biological evaluation of novel chalcones tethered triazolo[3,4- a ]isoquinoline as EGFR inhibitors targeting resistance in non-small cell lung cancer

doi: 10.1038/s41598-024-76459-x

Figure Lengend Snippet: Selectivity index of the tested compounds 3e and 3f relative to the drugs for EGFR proteins: EGFR L858R , EGFR T790M , and EGFR wt . Selectively index (SI a ) = EGFR WT IC 50 / EGFR L858R IC 50 value, Selectively index (SI b ) = EGFR WT IC 50 / EGFR T790M IC 50 value.

Article Snippet: The EGFR proteins (EGFR WT , EGFR T790M and EGFR L858R ) were obtained from Signalchem (BC V6V 2J2, Canada).

Techniques: