Journal: PLoS ONE

Article Title: Measuring EGFR Separations on Cells with ∼10 nm Resolution via Fluorophore Localization Imaging with Photobleaching

doi: 10.1371/journal.pone.0062331

Figure Lengend Snippet: (A) Single-molecule TIRF image of glass-immobilised 13.3 nm DNA rulers, labelled at each end with Atto 647 N. (B) Single-molecule TIRF image of T47D cells labelled with anti-EGFR Affibody-Atto 647 N. Scale bar = 8 µm.

Article Snippet: Protein S resin (Sigma) was washed twice with PBS and incubated with anti-EGFR antibody (Cell Signaling Technology #2256) for 1 h at room temperature, then washed twice with PBS to remove unbound antibody.

Techniques:

Journal: PLoS ONE

Article Title: Measuring EGFR Separations on Cells with ∼10 nm Resolution via Fluorophore Localization Imaging with Photobleaching

doi: 10.1371/journal.pone.0062331

Figure Lengend Snippet: (A) Western blots of A431 cell immunoprecipitates (I) and whole cell lysates (C) from unstimulated cells (blank) and cells stimulated with either 100 nM EGF or 2 nM EGFR Affibody. Proteins were probed (left to right) for non-specific tyrosine phosphorylation of proteins (Anti-pY4G10), tyrosine phosphorylation of EGFR (Anti-pY1045 EGFR) to show activated receptor, and presence of EGFR (Anti-EGFR). (B) Results of densitometry normalized against the total EGFR and relative quantity expressed as a fold change compared to an unstimulated control.

Article Snippet: Protein S resin (Sigma) was washed twice with PBS and incubated with anti-EGFR antibody (Cell Signaling Technology #2256) for 1 h at room temperature, then washed twice with PBS to remove unbound antibody.

Techniques: Western Blot, Phospho-proteomics, Control

Journal: Cell Death & Disease

Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers

doi: 10.1038/s41419-024-06916-y

Figure Lengend Snippet:

Article Snippet: Phospho-EGFR , 1:1000 , Cell Signaling, 1068S.

Techniques:

Journal: Cell Death & Disease

Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers

doi: 10.1038/s41419-024-06916-y

Figure Lengend Snippet:

Article Snippet: Phospho-EGFR , 1:1000 , Cell Signaling, 1068S.

Techniques:

Journal: PLoS ONE

Article Title: Pulmonary Neoplasms in Patients with Birt-Hogg-Dubé Syndrome: Histopathological Features and Genetic and Somatic Events

doi: 10.1371/journal.pone.0151476

Figure Lengend Snippet: Summary of histology, gene mutations and immunostaining.

Article Snippet: For immunostaining, the following reagents were used: rabbit polyclonal antibodies against phospho-mTOR (p-mTOR) (Ser2448) (Cell Signaling Technology, Danvers, MA), phospho-S6 ribosomal protein (p-S6) (Ser235/236) (Cell Signaling Technology), phospho-Akt (p-Akt) (Ser473) (Cell Signaling Technology), and FLCN (ab93196, Abcam, Cambridge, UK); rabbit monoclonal mutant/deleted-specific antibody against EGFR (L858R and E746-A750 deletion) (Cell Signaling Technology); rabbit monoclonal antibody against ROS1 (clone D4D6, Cell Signaling Technology); and mouse monoclonal antibody against ALK (clone 5A4, Abcam).

Techniques: Immunostaining, Mutagenesis, Labeling

Journal: PLoS ONE

Article Title: Pulmonary Neoplasms in Patients with Birt-Hogg-Dubé Syndrome: Histopathological Features and Genetic and Somatic Events

doi: 10.1371/journal.pone.0151476

Figure Lengend Snippet: (A) Germline and somatic FLCN status in 3 representative cases with different mutation patterns are shown. Control normal sequences are shown on the left. Germline mutations are shown on the middle. The somatic status of FLCN in microdissected neoplasms are shown on the right. (B) The papillary adenocarcinoma (PAC) had a heterozygous missense mutation (L858R) in EGFR (indicated by an arrow). (C) The micropapillary adenocarcinoma (MPAC) had a heterozygous missense mutation (G12D) in KRAS (right, indicated by an arrow).

Article Snippet: For immunostaining, the following reagents were used: rabbit polyclonal antibodies against phospho-mTOR (p-mTOR) (Ser2448) (Cell Signaling Technology, Danvers, MA), phospho-S6 ribosomal protein (p-S6) (Ser235/236) (Cell Signaling Technology), phospho-Akt (p-Akt) (Ser473) (Cell Signaling Technology), and FLCN (ab93196, Abcam, Cambridge, UK); rabbit monoclonal mutant/deleted-specific antibody against EGFR (L858R and E746-A750 deletion) (Cell Signaling Technology); rabbit monoclonal antibody against ROS1 (clone D4D6, Cell Signaling Technology); and mouse monoclonal antibody against ALK (clone 5A4, Abcam).

Techniques: Mutagenesis, Control