Journal: Open biology

Article Title: MYH7 R453C induced cardiac remodelling via activating TGF-β/Smad2/3, ERK1/2 and Nox4/ROS/NF-κB signalling pathways.

doi: 10.1098/rsob.230427

Figure Lengend Snippet: Figure 8. Effects of EGCG on the MYH7 R453C mutation H9C2 cells. (a) Effects of EGCG on the MYH7 R453C mutation H9C2 cell survival rate. (b) Protective effects of EGCG in MYH7 R453C mutation on ROS production. (c) Effect of EGCG on Bax and Bcl-2 activities of the MYH7 R453C mutation H9C2 cells. (d) Bcl-2 expression were quantified by densitometry and normalized to GAPDH levels. (e) Bax expression was quantified by densitometry and normalized to GAPDH levels. (f) Quantitative analysis of the ratio of Bcl-2 to Bax in protein expression was evaluated. Data are expressed as the mean ± s.d. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n = 3 biologically independent samples.

Article Snippet: Epigallocatechin gallate Epigallocatechin gallate (EGCG) was purchased from MedChem Express (MCE, USA, HY-13653) with a purity of 99.87%.

Techniques: Mutagenesis, Expressing

Journal: iScience

Article Title: Excitatory amino acid transporter 1 supports adult hippocampal neural stem cell self-renewal

doi: 10.1016/j.isci.2023.107068

Figure Lengend Snippet:

Article Snippet: EGCG (50 μM) , Tocris , Cat#4524.

Techniques: Virus, Plasmid Preparation, Recombinant, RNA HS Assay, Cell Culture, Derivative Assay, Software

Journal: The Journal of nutritional biochemistry

Article Title: Therapeutic properties of green tea against environmental insults

doi: 10.1016/j.jnutbio.2016.05.005

Figure Lengend Snippet: Green tea and smoke-related injury

Article Snippet: Syed, 2007 [ 77 ] , Normal human bronchial epithelial cells , EGCG (>98% pure) (Mitsui Norin Co., Ltd Shizuoka, Japan) , Smoke Smoke+EGCG , ↓ Smoke-induced cell proliferation ↓ Smoke-induced activation of NF-κB, ↓ NF-κB-regulated proteins cyclin D1, MMP-9, IL-8, iNOS ↓ Smoke-induced phosphorylation of ERK1/2, JNK, p38 MAPK.

Techniques: Suspension, Control, Phospho-proteomics, Activation Assay, Activity Assay, Marker, Concentration Assay, Expressing, Residue

Journal: Advanced Healthcare Materials

Article Title: A Dual‐Layer Hydrogel Barrier Integrating Bio‐Adhesive and Anti‐Adhesive Properties Prevents Postoperative Abdominal Adhesions

doi: 10.1002/adhm.202405238

Figure Lengend Snippet: Schematic illustration for fabrication of bilayer HPPT hydrogel barrier. In A), The inner layer was synthesized by coaercevate formation of TA and PEI resulting in intramolecular hydrophobic and intermolecular H‐bonding. B) The bioadhesive when applied on injured site repels water. The outer layer is formed by C) EGCG‐PBA complex which was conjugated with D) HA‐SPDA polymer and amine terminal pNiPAM, 4‐(4,6‐dimethoxy‐1,3,5‐triazin‐2‐yl)‐4‐methylmorpholinium chloride (DMTMM) at RT for 3 days to form HSPE‐pNiPAM microgel, and was mixed with PVA to form HP hydrogel within 10s. E) The anti‐adhesive, zwitterionic HP hydrogel outer layer was applied, to prevent adhesion. This schematic illustration was developed using BioRender.com, in compliance with the terms of an authorized license.

Article Snippet: EGCG (18.9 mg; 0.125 M; AstaTech) and 3‐aminophenylboronic acid (PBA; 62.1 mg; 1 M; Sigma‐Aldrich) were dissolved in 300 μL DMSO and then left at RT for 20 min before being added to the HA‐SPDA solution, where it was allowed to react for an additional 40 minutes.

Techniques: Synthesized, Polymer, Adhesive