Journal: Cell stem cell

Article Title: Deconstructing Olfactory Stem Cell Trajectories at Single Cell Resolution

doi: 10.1016/j.stem.2017.04.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Chemicals, Peptides, and Recombinant Proteins tamoxifen Sigma T5648 Neurobasal medium Thermo Fisher Scientific 21103049 papain Worthington Biochemical Corporation {"type":"entrez-nucleotide","attrs":{"text":"LS003119","term_id":"1321651605","term_text":"LS003119"}} LS003119 fetal bovine serum Thermo Fisher Scientific 26140-087 propidium iodide Sigma 81845 tissue freezing medium Fisher 15-183-13 goat serum Jackson ImmunoResearch 005000121 donkey serum Jackson ImmunoResearch 017000121 Hoechst 33342 Thermo Fisher Scientific H3570 DAPI in Vectashield Vector Laboratories H-1500 5-ethynyl-2′-deoxyuridine (EdU) Thermo Fisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"A10044","term_id":"489105","term_text":"A10044"}} A10044 Dig RNA labeling mix Roche (sold by Sigma) 1277073 RNA Labeling Mix, Fluorescein Roche (sold by Sigma) 11685619910 BCIP/NBT color development substrate Promega s3771 SuperScript RTIII reverse transcriptase Invitrogen (now Thermo Fisher Scientific) 18080093 Trizol Invitrogen (now Thermo Fisher Scientific) 10296-010 DNase1 Roche 03539121103 Critical Commercial Assays C1 IFC for mRNA-seq (5–10 μM) Fluidigm 1005759 C1 IFC for mRNA-seq (10–17 μM) Fluidigm 1005760 C1 Single-Cell Auto Prep Kit for mRNA Seq Fluidigm 100-6209 C1 Single-Cell Auto Prep Reagent Kit for mRNA Seq Fluidigm 100-6201 SMARTer Ultra Low RNA Kit for the Fluidigm C1 System Clontech 634833 Advantage 2 PCR Kit Clontech 639207 Agilent High Sensitivity DNA Kit Reagents Agilent 5067-4626 Nextera XT DNA Sample Preparation Kit Illumina FC-131-1096 Nextera XT DNA Sample Preparation Index Kits A–D Illumina FC-131-2001-4 Agencourt AMPure XP beads Beckman Coulter {"type":"entrez-nucleotide","attrs":{"text":"A63880","term_id":"3717426","term_text":"A63880"}} A63880 Qubit dsDNA High Sensitivity Assay Kit Life Technologies Molecular Probes {"type":"entrez-protein","attrs":{"text":"Q32854","term_id":"75280861","term_text":"Q32854"}} Q32854 Tyramide Signal Amplification kit cy3 Perkin-Elmer NEL744001KT Tyramide Signal Amplification kit FITC Perkin-Elmer NEL741001KT Click-iT EdU Imaging Kit, Alex Fluor 647 Thermo Fisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"C10340","term_id":"1535411","term_text":"C10340"}} C10340 Deposited Data Raw data, normalized counts matrix GEO GEO accession # {"type":"entrez-geo","attrs":{"text":"GSE95601","term_id":"95601"}} GSE95601 Scripts for analysis GitHub https://github.com/rufletch/p63-HBC-diff Experimental Models: Cell Lines None.

Techniques: Recombinant, Plasmid Preparation, Labeling, Sample Prep, Sensitive Assay, Amplification, Imaging, Software