Journal: Journal of vascular research

Article Title: A Novel Model of Balloon Angioplasty Injury in Rat Arteriovenous Fistula

doi: 10.1159/000507080

Figure Lengend Snippet: (A) α-SMA and CD68 immunostaining of representative sections from Group 1 and Group 2. Scale bar=100 μM. Higher magnification images are at 40X. (B) Quantification of α-SMA (intimal region) and CD68 (intimal and medial regions) expression from AVF-artery and AVF-vein. N=3–4 in each group. *p<0.05, **p<0.001. An unpaired t-test was used to test for statistical differences between AVF without angioplasty and AVF with angioplasty groups.

Article Snippet: Immunohistochemical Analysis Paraffin embedded arterial and venous sections were evaluated to identify the cells contributing to the vascular remodeling following balloon angioplasty. α-SMA (14-9760-82, Invitrogen) and CD68 (NB600–985, Novus Biologicals) primary antibodies were used to verify the presence of smooth muscle cell phenotype and inflammatory cells respectively. α-SMA expression in the intimal region and CD68 expression in both medial and intimal regions of AVF vessels were quantified using Cellsense Dimension Software (Olympus Life Science) on digital photographs taken at a final magnification of 20X.

Techniques: Immunostaining, Expressing

Journal: Physiological Reports

Article Title: Autologous minced muscle grafts improve endogenous fracture healing and muscle strength after musculoskeletal trauma

doi: 10.14814/phy2.13362

Figure Lengend Snippet: Flow cyotometry antibodies

Article Snippet: CD68 , ED1 , Novus Biologicals , NB600‐985V , 0.001 mg/mL.

Techniques: Concentration Assay

Journal: Journal of Inflammation Research

Article Title: Discovery and Development of a Novel mPGES-1/5-LOX Dual Inhibitor LFA-9 for Prevention and Treatment of Chronic Inflammatory Diseases

doi: 10.2147/jir.s286110

Figure Lengend Snippet: Figure 5 Anti-inflammatory activity of LFA-9. Paw thickness (A) and percent inhibition (B) using inflammogen (carrageenan (CAR))-induced rat paw edema. (C) Effect of LFA-9 on PGE2, LTB4, PGI2, and TXB2 levels in exudates of carrageenan (CAR))-induced rat paw edema. (D) Effect of LFA-9 on expression of CD68 and TNF-α in CAR- injected paw tissue using immunoblot analysis. (E and F) Effect of LFA-9 on CD68-positive inflammatory cell infiltration and TNF-α expression in CAR-injected paw tissue sections using immunofluorescence technique. P-values ≤0.05 for data sets were considered significant. (NS) Not significant (P>0.05); *(P ≤ 0.05); **(P ≤ 0.01); *** (P ≤ 0.001); **** (P ≤ 0.0001). (G) Schematic representation of mechanism of action of LFA-9 for its anti-inflammatory activity with safety.

Article Snippet: After incubation with primary antibody [Alexa Fluor 700-conjugated CD68 (1:50 dilution) (Novus Biologicals (NB600985)) and TNF-α (1:100 dilution) (Santa Cruz)] overnight at 4°C, slides were labeled with Alexa Fluor 488 dye-conjugated secondary antibody (for TNF-α) and mounted with ProLong Gold (Thermo Fisher Scientific).

Techniques: Activity Assay, Inhibition, Expressing, Injection, Western Blot, Immunofluorescence

Journal: iScience

Article Title: A rat liver cell atlas reveals intrahepatic myeloid heterogeneity

doi: 10.1016/j.isci.2023.108213

Figure Lengend Snippet:

Article Snippet: Mouse anti-rat CD68 AF700; Clone: ED1 , Novus Biologicals , Cat# NB600-985AF700.

Techniques: Recombinant, SYBR Green Assay, Staining, Single Cell, Selection, Gene Expression, Software