Journal: BMC Plant Biology

Article Title: Genome-wide association study of agronomic traits in winter wheat ( Triticum aestivum L.) using a custom SNP marker set

doi: 10.1186/s12870-025-07322-y

Figure Lengend Snippet: Proportion of SNPs of the final 3.5 K set for which significant marker-trait associations were found. The SNP groups are as follows: A based on KASP MAS markers, B from previous GWAS that use iSelect-90 K array, C mutations in genes which sequences of were deposited to NCBI GenBank database, D protein-changing mutations, G random SNPs for gap filling. Letters above the diagram designate homogenous groups of non-significantly different values according to Fishers exact test (FET)

Article Snippet: The PCR mixture included 5 μl of the master mix, containing FAM, HEX and ROX fluorescent dyes (KASP TF V4.0 2X Master Mix, KBS-1050-102, LCG Biosearch Techhnology, Teddington, UK), 5 μl of matrix DNA at a concentration of 15–30 ng/μl, and 0.14 μl primer mix for each SNP (Additional file 1, Supplementary Table 2).

Techniques: Marker