Journal: Poultry Science

Article Title: Duck enteritis virus LORF4 gene is a late gene and nonessential for virus replication in vitro

doi: 10.1016/j.psj.2024.104275

Figure Lengend Snippet: The intracellular localization of pLORF4 in DEF infected with DEV. Immunofluorescence microscopy images of pLORF4 (red) at 24, 36, 48 and 60 hpi, DAPI stained cell nuclei (blue), DEF fluorescence microscopy images after merging (MERGE) (scale bar, 20 μ m), uninfected DEF (Mock), and finally captured on a Nikon fluorescence microscope (TS2FL), the images were processed with NIS Elements software.

Article Snippet: Immunofluorescence microscopy images of pLORF4 (red) at 24, 36, 48 and 60 hpi, DAPI stained cell nuclei (blue), DEF fluorescence microscopy images after merging (MERGE) (scale bar, 20 μ m), uninfected DEF (Mock), and finally captured on a Nikon fluorescence microscope (TS2FL), the images were processed with NIS Elements software.

Techniques: Infection, Immunofluorescence, Microscopy, Staining, Fluorescence, Software

Journal: Nature Communications

Article Title: Serum-tolerant polymeric complex for stem-cell transfection and neural differentiation

doi: 10.1038/s41467-025-57278-8

Figure Lengend Snippet: a Representative fluorescent images of MSCs after FAM-miRNA (green) transfection with 25K PEI FAM-miRNA , 10K PEI FAM-miRNA , BP FAM-miRNA , and APOs@BP FAM-miRNA in medium with or without 10% FBS. b Cell viability after transfection with 25k PEI miRNA , 10k PEI miRNA , BP miRNA , and APOs@BP miRNA in medium with or without 10% FBS. c Cell proliferation in 3 days after transfection with 25K PEI miRNA , 10K PEI miRNA , BP miRNA , and APOs@BP miRNA in medium with 10% FBS. d Representative TEM images of 25K PEI miRNA , 10K PEI miRNA , BP miRNA , and APOs@BP miRNA after incubation in 10% FBS for 2 h. e Evaluation of the stability of 25K PEI miRNA , 10K PEI miRNA , BP miRNA, and APOs@BP miRNA in the presence or absence of serum by measuring particle size, respectively. f Determination of protein adsorption of polymeric complexes by SDS-PAGE. g Schematic illustration of serum-tolerant polymeric complexes to avoid serum-protein adsorption . h SPR analysis of MSC membrane binding affinity of BSA@BP miRNA and APOs@BP miRNA i CLSM images of lipid vesicles (labeled with DiI, red) after incubation with FAM labeled BSA@BP miRNA and APOs@BP miRNA (green), respectively. The solid white line denotes the straight-line distance employed for colocation analysis between DiI and FAM signals on the right. j The conformational content of APOs in APOs@BP miRNA before and after incubation with lipid vesicles. k Schematic illustration of intracellular trafficking of APOs@BP miRNA . l The dynamic colocalization of APOs@BP Cy5-miRNA (green) and lysosomes (red) in MSCs after incubation for 0.5 and 2 h, respectively. m Intracellular accumulation and ROS-triggered dissociation of Cy5-miRNA (red) from polymerized carrier (FBP, green). Data were presented as mean ± SD ( n = 5 independent samples in b ; n = 6 independent samples in c ; n = 3 independent samples in e , j ;). Two-tailed unpaired t -test was used for statistical analysis of b , j . One-way ANOVA with Dunnett’s multiple comparisons test was used for statistical analysis of c . Three independent experiments were performed with similar results and representative results are shown. Source data are provided as a Source Data file.

Article Snippet: Different polymers, including 10K PEI, 25K PEI, BP, and APOs@BP, compressed FAM-labeled miRNA (FAM-miRNA, RIBOBIO, miR01104-1-1) at their optimized N/P ratios and dissolved in DMEM containing 0 or 10% FBS to maintain a final miRNA concentration of 120 nM, which were then incubated with MSCs for 6 h. The transfected MSCs were washed three times with PBS and observed using an inverted fluorescence microscope (Eclipse Ts2-FL, Nikon Precision Inc., CA, USA).

Techniques: Transfection, Incubation, Adsorption, SDS Page, Membrane, Binding Assay, Labeling, Two Tailed Test