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Image Search Results
Journal: Cellular and Molecular Bioengineering
Article Title: Multicompartmentalized Microvascularized Tumor-on-a-Chip to Study Tumor-Stroma Interactions and Drug Resistance in Ovarian Cancer
doi: 10.1007/s12195-024-00817-y
Figure Lengend Snippet: Microfluidic device mimics drug penetration gradients in OC-TME. a Schematic representation of method used for doxorubicin penetration assay, where doxorubicin (100 µM) was injected in both circulation channels and images were taken in time-lapse fluorescent microscope up to 24 h. b Representative images at 5 min, 30 min, 2, 4, 7 and 24 h of doxorubicin penetration. Scale Bar = 1 mm. c Quantification of the fluid velocity (μm/s) inside the microfluidic device over the time (h) (left) and MFI of doxorubicin penetration inside the cancer and stromal chambers at each time point (right). d Schematic representation of method used for doxorubicin uptake, cancer cells were cultured surrounded by different stromal cells (EC, NF, or CAF) or blank scaffold as a control for 5 days and then doxorubicin (100 µM) was injected in both circulation channels and images were taken at 24 h. e Representative images of doxorubicin uptake by cells at 24 h. Scale Bar = 1 mm. Figure a and d created with Biorender.com
Article Snippet: The images were taken at different time points (0; 0.25; 0.5; 0.75, 1; 1.25; 1.5; 1.75; 2, 2.5; 3; 4; 5; 6; 7; 8; 24 hours) for drug penetration and at 24 h for drug uptake using
Techniques: Injection, Microscopy, Cell Culture, Control