Journal: Food Hydrocolloids

Article Title: Impact of polyelectrolyte complex layer on the stability of palm oil multiple emulsions encapsulating a water-soluble compound during heating, cooling, and storage processes

doi: 10.1016/j.foodhyd.2022.108335

Figure Lengend Snippet: Fig. 5. Top views of the emulsion samples in the absence and presence of a CHI–SC complex layer during storage at 25 and 4 ◦C, taken with a digital microscope. Scale bars represent 1000 μm.

Article Snippet: Polarized microscopic observations were performed using a polarized optical microscope (ECLIPSE Ci POL; Nikon Solutions Co., Ltd., Tokyo, Japan).

Techniques: Emulsion, Microscopy

Journal: Food Hydrocolloids

Article Title: Impact of polyelectrolyte complex layer on the stability of palm oil multiple emulsions encapsulating a water-soluble compound during heating, cooling, and storage processes

doi: 10.1016/j.foodhyd.2022.108335

Figure Lengend Snippet: Fig. 8. (a) Polarized photomicrographs of W/O/W emulsions at 20 ◦C at different storage times. (b) Time courses of φc of palm oil multiple droplets determined by polarized microscope observation.

Article Snippet: Polarized microscopic observations were performed using a polarized optical microscope (ECLIPSE Ci POL; Nikon Solutions Co., Ltd., Tokyo, Japan).

Techniques: Microscopy

Journal: Pharmaceutics

Article Title: Micelle-Forming Block Copolymers Tailored for Inhibition of P-gp-Mediated Multidrug Resistance: Structure to Activity Relationship

doi: 10.3390/pharmaceutics11110579

Figure Lengend Snippet: The schematic structure of amphiphilic block copolymers based on PHPMA and PPO: (X) = H, diblock copolymer; (X) = PHPMA, triblock copolymer; (Y) = Boc, Boc-protected copolymer; (Y) = H, deprotected copolymer with free NHNH 2 groups.

Article Snippet: The molecular weight of the formed micelles and the ratio between micellar and unimer form of copolymers were determined by FFF (Eclipse 3+ modul) equipped with DLS (DAWN 8+; Wyatt Technology Corp., Santa Barbara, CA, USA), UV and RI (Optilab rEX;Wyatt Technology Corp., Santa Barbara, CA, USA) detection using a regenerated cellulose membrane (10 kDa) for the analysis and a mixture of water/NaN 3 as the mobile phase.

Techniques: Blocking Assay

Journal: Pharmaceutics

Article Title: Micelle-Forming Block Copolymers Tailored for Inhibition of P-gp-Mediated Multidrug Resistance: Structure to Activity Relationship

doi: 10.3390/pharmaceutics11110579

Figure Lengend Snippet: Chromatograms of molar weights of diblock P4 (red curve) and triblock P6 (blue curve) copolymers determined by FFF (3 mg mL −1 ; mobile phase: water/NaN 3 ; 10 kDa regenerated cellulose).

Article Snippet: The molecular weight of the formed micelles and the ratio between micellar and unimer form of copolymers were determined by FFF (Eclipse 3+ modul) equipped with DLS (DAWN 8+; Wyatt Technology Corp., Santa Barbara, CA, USA), UV and RI (Optilab rEX;Wyatt Technology Corp., Santa Barbara, CA, USA) detection using a regenerated cellulose membrane (10 kDa) for the analysis and a mixture of water/NaN 3 as the mobile phase.

Techniques:

Journal: Pharmaceutics

Article Title: Micelle-Forming Block Copolymers Tailored for Inhibition of P-gp-Mediated Multidrug Resistance: Structure to Activity Relationship

doi: 10.3390/pharmaceutics11110579

Figure Lengend Snippet: Dependence of fluorescent intensity on the concentration of the triblock and diblock micelles; fluorescent intensity of fluorescent dye Nile Red of diblock copolymers determined after 30 min at λ ex /λ em = 530/610 nm in PBS buffer at 37 °C. The CMC was determined as the intersection of two straight lines drawn through points at low and high concentration from the graph of dependence of fluorescence intensity on negative logarithm of sample concentration.

Article Snippet: The molecular weight of the formed micelles and the ratio between micellar and unimer form of copolymers were determined by FFF (Eclipse 3+ modul) equipped with DLS (DAWN 8+; Wyatt Technology Corp., Santa Barbara, CA, USA), UV and RI (Optilab rEX;Wyatt Technology Corp., Santa Barbara, CA, USA) detection using a regenerated cellulose membrane (10 kDa) for the analysis and a mixture of water/NaN 3 as the mobile phase.

Techniques: Concentration Assay, Fluorescence

Journal: Pharmaceutics

Article Title: Micelle-Forming Block Copolymers Tailored for Inhibition of P-gp-Mediated Multidrug Resistance: Structure to Activity Relationship

doi: 10.3390/pharmaceutics11110579

Figure Lengend Snippet: The inhibitory activity of block copolymer carriers in P388/MDR cells determined by calcein efflux assay. ( A ) The influence of Boc-deprotection on P-gp inhibition in P388/MDR cells after 24 h of cultivation (5% CO 2 , 37 °C) with titrated concentrations of block copolymers containing indicated amount of PPO; 10 µM cyclosporine A as the positive control; ( B ) The effect of micelle-forming copolymers on viability of P388/MDR cells determined using Hoechst 33258. Cell viability after 24 h of cultivation with Boc-protected and or Boc-deprotected copolymers; ( C ) The ability of Boc-deprotected diblock P4 and triblock P6 copolymers to inhibit P-gp function.

Article Snippet: The molecular weight of the formed micelles and the ratio between micellar and unimer form of copolymers were determined by FFF (Eclipse 3+ modul) equipped with DLS (DAWN 8+; Wyatt Technology Corp., Santa Barbara, CA, USA), UV and RI (Optilab rEX;Wyatt Technology Corp., Santa Barbara, CA, USA) detection using a regenerated cellulose membrane (10 kDa) for the analysis and a mixture of water/NaN 3 as the mobile phase.

Techniques: Activity Assay, Blocking Assay, Inhibition, Positive Control

Journal: Pharmaceutics

Article Title: Micelle-Forming Block Copolymers Tailored for Inhibition of P-gp-Mediated Multidrug Resistance: Structure to Activity Relationship

doi: 10.3390/pharmaceutics11110579

Figure Lengend Snippet: The P-gp inhibitory activity of PHPMA-PPO copolymers with different proportion of free PPO. The P388/MDR cells were cultivated for 24 h with titrated concentrations of copolymer samples and their P-gp activity determined by calcein assay; 10 µM cyclosporine A and sensitive P388 cells with calcein as the positive control ( A ) Samples resulting from adding various wt % of free PPO to the original sample P4, in which the total PPO content was determined by NMR. The x -axis indicates concentrations of PPO before loading of the free PPO, amount of the added free PPO as indicated in the legend; ( B ) Inhibitory activity of a series of copolymers (P4, P7, P8, P9, P11, and P14) with various ratios of PHPMA-PPO to the loaded free PPO. The x -axis indicates total content of PPO in the samples, which is the sum of the starting PPO in the original sample and the added free PPO; ( C ) Viability of P388/MDR cells cultivated with titrated concentrations of copolymer samples with various ratio of PHPMA-PPO to the loaded free PPO as shown in B. Viable cells (Hoechst 33258-negative) were determined by flow cytometry. ( D ) Inhibitory activity of the mixture of PHPMA-PPO copolymer and added free PHPMA in a molar ratio of 1:1.

Article Snippet: The molecular weight of the formed micelles and the ratio between micellar and unimer form of copolymers were determined by FFF (Eclipse 3+ modul) equipped with DLS (DAWN 8+; Wyatt Technology Corp., Santa Barbara, CA, USA), UV and RI (Optilab rEX;Wyatt Technology Corp., Santa Barbara, CA, USA) detection using a regenerated cellulose membrane (10 kDa) for the analysis and a mixture of water/NaN 3 as the mobile phase.

Techniques: Activity Assay, Positive Control, Flow Cytometry

Journal: Pharmaceutics

Article Title: Micelle-Forming Block Copolymers Tailored for Inhibition of P-gp-Mediated Multidrug Resistance: Structure to Activity Relationship

doi: 10.3390/pharmaceutics11110579

Figure Lengend Snippet: The activity of block copolymer carriers in sensitive P388 cells determined by calcein efflux assay. ( A ) The effect of Boc-protected or -deprotected copolymers on P-gp inhibition after 24 h of; 10 µM cyclosporine A as the positive control. ( B ) The effect of PHPMA-PPO copolymers with loaded free PPO.

Article Snippet: The molecular weight of the formed micelles and the ratio between micellar and unimer form of copolymers were determined by FFF (Eclipse 3+ modul) equipped with DLS (DAWN 8+; Wyatt Technology Corp., Santa Barbara, CA, USA), UV and RI (Optilab rEX;Wyatt Technology Corp., Santa Barbara, CA, USA) detection using a regenerated cellulose membrane (10 kDa) for the analysis and a mixture of water/NaN 3 as the mobile phase.

Techniques: Activity Assay, Blocking Assay, Inhibition, Positive Control