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Image Search Results
Journal: PLoS ONE
Article Title: Targeting Angiogenesis-Dependent Calcified Neoplasms Using Combined Polymer Therapeutics
doi: 10.1371/journal.pone.0005233
Figure Lengend Snippet: Free (open squares/triangles/diamonds) and polymer-conjugated ALN and TNP-470 (closed squares/triangles/diamonds) had similar effect on cell proliferation exhibiting retention of activity following polymer conjugation. Free and conjugated ALN and TNP-470 inhibited ECGS-induced HUVEC proliferation at IC 50 of 0.7 and 1 nM respectively. Free and conjugated drugs had cytotoxic effect on these cells at doses >1 and 10 nM respectively. The inhibition of endothelial proliferation by the conjugate was reduced significantly (IC 50 = 4200 nM) in the presence of cathepsin K inhibitor III (open circles). Proliferation of Saos-2 and MG-63-Ras cells was inhibited by free and conjugated ALN and TNP-470 at higher concentrations with IC 50 of 30 µM and 10 µM respectively. The solid line represents the proliferation of growth factor-induced cells and the dotted line represents the cell proliferation in the absence of ECGS. Data represent mean±s.d. * P <0.05, ** P <0.03, ** * P <0.05 compared with control.
Article Snippet: EGM-2 medium was from Cambrex, USA and
Techniques: Activity Assay, Conjugation Assay, Inhibition