Journal: Naunyn-Schmiedeberg's Archives of Pharmacology

Article Title: Enhancement of cardiac angiogenesis in a myocardial infarction rat model using selenium alone and in combination with PTXF: the role of Akt/HIF-1α signaling pathway

doi: 10.1007/s00210-023-02904-9

Figure Lengend Snippet: ECG changes in rats with MI induced by isoprenaline (ISP) and treated orally with selenium (Se), pentoxifylline (PTXF), or their combination (Se + PTXF). A ECG graph. B ST segment elevation. Results are expressed as mean ± SD ( n = 6). ST-segment elevation (orange arrow)

Article Snippet: An ECG PowerLab module and a Bio-Amplifier (AD Instruments, Australia) were used for ECG monitoring.

Techniques:

Journal: JACC: Basic to Translational Science

Article Title: Loss of YTHDF2 Alters the Expression of m 6 A-Modified Myzap and Causes Adverse Cardiac Remodeling

doi: 10.1016/j.jacbts.2023.03.012

Figure Lengend Snippet: Loss of YTHDF2 Causes Cardiac Dysfunction and Fibrosis (A) Fractional shortening (%) by echocardiography of the littermate control animals and Y2-cKO mice. (B) M-mode echocardiographic measurement of interventricular septal thickness in diastole (IVS,d) as a marker of left ventricular anterior wall thickness. (C) Quantification of fibrosis by Picrosirius red stain. (D) Representative images for Picrosirius red stain of heart cross-sections (fibrosis [red] ). Scale bar = 1,000 μm for the full-sized images and 250 μm for the callouts. (E to G) Electrocardiography parameters recorded during baseline monitoring of the littermate controls and Y2-cKO mice. The values represent means calculated using LabChart Pro ECG Analysis throughout the 5-minute period of recording: (E) heart rates (beats/min), (F) QRS intervals (seconds), and (G) QTc intervals (seconds) adjusted to heart rates. (H, I) Results of the electrocardiogram (ECG) monitoring 15 minutes after epinephrine and caffeine injections. (H) Percent of mice of each genotype which developed either no events or ventricular arrhythmic events in order of increasing event severity (y-axis): isolated premature ventricular contractions (PVCs), frequent PVCs, bigeminy, or ventricular tachycardia. (I) Assigned worst arrhythmia scores to each mouse were based on the worst ventricular arrhythmia displayed and adapted from van der Werf et al : 0 = no events; 1 = isolated PVCs (<10 per minute); 2 = frequent PVCs (≥10 per minute); 3 = ventricular bigeminy; 4 = ventricular tachycardia. Normally distributed data were compared using the unpaired 2-tailed t test with Welch's correction for unequal variances or Wilcoxon rank sum test for skewed data: ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001. Incidence of arrhythmic events in the 2 groups was compared by Fisher exact test. Data are presented as the mean ± SEM with the individual data points shown. Group sizes are listed in order of control and Y2-cKO mice: (A, B) n = 18, 10; (C) n = 11, 6; (E to G) n = 11, 13: (I) n = 7, 13. Abbreviations as in Figure 1 .

Article Snippet: The baseline recording was performed for 5 minutes, and the standard parameters (heart rates; P duration; PR, QRS, QT, JT, QTc, JTc, and RR intervals; ST height; and all wave amplitudes) were calculated using LabChart Pro ECG Analysis v2.3.2 module (ADInstruments).

Techniques: Marker, Staining, Isolation

Journal: bioRxiv

Article Title: Loss of developmentally derived Irf8+ macrophages promotes hyperinnervation and arrhythmia in the adult zebrafish heart

doi: 10.1101/2024.04.17.589909

Figure Lengend Snippet: (A) Cartoon depiction of the rack-compatible swim tunnel with metered flow. Following a 5-minute acclimation period within the tunnel, 12 mpf zebrafish were subjected to increasing flow rate intervals for a total of 1 hour. (B) Cartoon depiction of an ECG recording and a representative ECG trace from an anesthetized fish placed ventral-side up in a sponge surrounded by anesthesia solution. (C) Representative ECG traces for wild type and irf8 mutant zebrafish following the swim assay. Black arrows indicate unique electrical disturbances occurring above any baseline noise. (D) Percentage of fish with an abnormal electrical event during the ECG recording. Male n = 7 per genotype, ****p < 0.0001. Female n = 4-8 per genotype, ****p < 0.0001. Fisher’s exact test. (E) Categorization of ECG abnormalities observed throughout all wild type and irf8 mutant fish.

Article Snippet: To record electrical impulses, we used the CardioPhys TM ECG system (World Precision Instruments), which included a low-noise gold ECG probe, micromanipulator, bio-amplifier and ground electrode, in combination with the PowerLab 2/26 data acquisition unit (ADInstruments, PL2602).

Techniques: Mutagenesis

Journal: bioRxiv

Article Title: Loss of developmentally derived Irf8+ macrophages promotes hyperinnervation and arrhythmia in the adult zebrafish heart

doi: 10.1101/2024.04.17.589909

Figure Lengend Snippet: (A) Cartoon depiction of an adult zebrafish undergoing an echocardiogram with the transducer in the long-axis (LAX) position. Echocardiograms were performed on unchallenged fish. (B) Cartoon of an adult zebrafish heart from the perspective of the echocardiography recording, denoting the atrium (A), atrioventricular (AV) valve, ventriculobulbar (VB) valve, and bulbus arteriosus (BA). The boxes around the AV valve and VB valve depict the gating strategies to determine inward flow and outward flow of blood from the ventricle. (C) Still image from an adult zebrafish echocardiography recording with the ventricle and bulbus arteriosus outlined in red. (D) Representative echocardiogram trace gating on the AV valve with the early filling (E) wave, late filling (A) wave, diastasis time, isovolumic contraction time (IVCT), aortic ejection time (AET), and isovolumic relaxation time (IVRT) labeled. (E-G) Quantifications of IVRT (E), AET (F), and pressure within the VB valve (G) in male and female wild type and irf8 mutant fish at 6 and 12 mpf. Male n = 10-21 per genotype and timepoint. Female n = 9-14 per genotype and timepoint. Each point represents individual beats per fish. 8-10 beats were analyzed per fish. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (H) Quantification of heart in male and female wild type and irf8 mutants. (I) Representative echocardiogram traces from a wild type and irf8 mutant recording. (J-M) Quantification of diastasis time in wild type males (J) and females (K) at 6 mpf and 12 mpf, as well as diastasis time in irf8 mutant males (L) and females (M) at 6 mpf and 12 mpf. A Gaussian distribution was created by analyzing 8 beats per sample and grouping diastasis duration in defined increments. Male n = 10-21 per genotype and timepoint. Female n = 9-14 per genotype and timepoint. Horizontal bars represent the range of diastasis duration. (N-O) 95% confidence interval of the average diastasis time durations in wild type (gray) and irf8 mutant (blue) males (N) and females (O).

Article Snippet: To record electrical impulses, we used the CardioPhys TM ECG system (World Precision Instruments), which included a low-noise gold ECG probe, micromanipulator, bio-amplifier and ground electrode, in combination with the PowerLab 2/26 data acquisition unit (ADInstruments, PL2602).

Techniques: Labeling, Mutagenesis

Journal: bioRxiv

Article Title: Loss of developmentally derived Irf8+ macrophages promotes hyperinnervation and arrhythmia in the adult zebrafish heart

doi: 10.1101/2024.04.17.589909

Figure Lengend Snippet: (A) Cartoon depiction of the rack-compatible swim tunnel with metered flow. Following a 5-minute acclimation period within the tunnel, 12 mpf zebrafish were subjected to increasing flow rate intervals for a total of 1 hour. (B) Cartoon depiction of an ECG recording and a representative ECG trace from an anesthetized fish placed ventral-side up in a sponge surrounded by anesthesia solution. (C) Representative ECG traces for wild type and irf8 mutant zebrafish following the swim assay. Black arrows indicate unique electrical disturbances occurring above any baseline noise. (D) Percentage of fish with an abnormal electrical event during the ECG recording. Male n = 7 per genotype, ****p < 0.0001. Female n = 4-8 per genotype, ****p < 0.0001. Fisher’s exact test. (E) Categorization of ECG abnormalities observed throughout all wild type and irf8 mutant fish.

Article Snippet: To record electrical impulses, we used the CardioPhys TM ECG system (World Precision Instruments), which included a low-noise gold ECG probe, micromanipulator, bio-amplifier and ground electrode, in combination with the PowerLab 2/26 data acquisition unit (ADInstruments, PL2602).

Techniques: Mutagenesis

Journal: bioRxiv

Article Title: Loss of developmentally derived Irf8+ macrophages promotes hyperinnervation and arrhythmia in the adult zebrafish heart

doi: 10.1101/2024.04.17.589909

Figure Lengend Snippet: (A) Cartoon depiction of an adult zebrafish undergoing an echocardiogram with the transducer in the long-axis (LAX) position. Echocardiograms were performed on unchallenged fish. (B) Cartoon of an adult zebrafish heart from the perspective of the echocardiography recording, denoting the atrium (A), atrioventricular (AV) valve, ventriculobulbar (VB) valve, and bulbus arteriosus (BA). The boxes around the AV valve and VB valve depict the gating strategies to determine inward flow and outward flow of blood from the ventricle. (C) Still image from an adult zebrafish echocardiography recording with the ventricle and bulbus arteriosus outlined in red. (D) Representative echocardiogram trace gating on the AV valve with the early filling (E) wave, late filling (A) wave, diastasis time, isovolumic contraction time (IVCT), aortic ejection time (AET), and isovolumic relaxation time (IVRT) labeled. (E-G) Quantifications of IVRT (E), AET (F), and pressure within the VB valve (G) in male and female wild type and irf8 mutant fish at 6 and 12 mpf. Male n = 10-21 per genotype and timepoint. Female n = 9-14 per genotype and timepoint. Each point represents individual beats per fish. 8-10 beats were analyzed per fish. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (H) Quantification of heart in male and female wild type and irf8 mutants. (I) Representative echocardiogram traces from a wild type and irf8 mutant recording. (J-M) Quantification of diastasis time in wild type males (J) and females (K) at 6 mpf and 12 mpf, as well as diastasis time in irf8 mutant males (L) and females (M) at 6 mpf and 12 mpf. A Gaussian distribution was created by analyzing 8 beats per sample and grouping diastasis duration in defined increments. Male n = 10-21 per genotype and timepoint. Female n = 9-14 per genotype and timepoint. Horizontal bars represent the range of diastasis duration. (N-O) 95% confidence interval of the average diastasis time durations in wild type (gray) and irf8 mutant (blue) males (N) and females (O).

Article Snippet: To record electrical impulses, we used the CardioPhys TM ECG system (World Precision Instruments), which included a low-noise gold ECG probe, micromanipulator, bio-amplifier and ground electrode, in combination with the PowerLab 2/26 data acquisition unit (ADInstruments, PL2602).

Techniques: Labeling, Mutagenesis