eb3 Search Results


90
Addgene inc fip1
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Santa Cruz Biotechnology eb3
Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of <t>EB3-GFP</t> live fluorescence imaging (B) and <t>EB3</t> staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page
Eb3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc memerald eb3 c 20 plasmid
Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of <t>EB3-GFP</t> live fluorescence imaging (B) and <t>EB3</t> staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page
Memerald Eb3 C 20 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pegfp centrin2 vectors
Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of <t>EB3-GFP</t> live fluorescence imaging (B) and <t>EB3</t> staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page
Pegfp Centrin2 Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc addgene plasmid
Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of <t>EB3-GFP</t> live fluorescence imaging (B) and <t>EB3</t> staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page
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Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of <t>EB3-GFP</t> live fluorescence imaging (B) and <t>EB3</t> staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page
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Addgene inc michael davidson plasmid collection
Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of <t>EB3-GFP</t> live fluorescence imaging (B) and <t>EB3</t> staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page
Michael Davidson Plasmid Collection, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc fugene 6
Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of <t>EB3-GFP</t> live fluorescence imaging (B) and <t>EB3</t> staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page
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Addgene inc michael davidson
Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of <t>EB3-GFP</t> live fluorescence imaging (B) and <t>EB3</t> staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page
Michael Davidson, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of EB3-GFP live fluorescence imaging (B) and EB3 staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page

Journal: eLife

Article Title: Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules

doi: 10.7554/elife.33864

Figure Lengend Snippet: Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of EB3-GFP live fluorescence imaging (B) and EB3 staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page

Article Snippet: We used rabbit polyclonal antibodies against CAMSAP2 (Novus, Littleton, CO, NBP1-21402), CEP135, acetylated tubulin, polyglutamylated tubulin and g-tubulin (Sigma-Aldrich, St Louis, MO, SAB4503685, T7451, T9822 and T3559), CDK5RAP2 (BethylLaboratories, Montgomery, TX, A300-554A), detyrosinated tubulin (Abcam, UK, ab48389), EB3 (Stepanova et al., 2003) and myomegalin isoform 8 (MMG8) (Wang et al., 2014), goat polyclonal antibodies against MYOSIN IIb and PCM1 (Santa-Cruz biotechnology, Dallas, TX, SC-47205 and SC-50164), mouse monoclonal anti- bodies against GM130, Pericentrin, EB1, VE-Cadherin, ZO-1, AKAP450 and KU80 (BD Biosciences, San Jose, CA, 610823, 611815, 610535, 610252, 610966, 611518 and 611360), CAMSAP3, a-tubulin and g-tubulin (Sigma-Aldrich, SAB4200415, T5168 and T6557), NEDD1 (Abnova, Taiwan, H00121441-M05) and rat monoclonal antibodies against a-tubulin YL1/2 (Pierce, Waltham, MA, MA1-80017).

Techniques: Migration, Imaging, Control, Staining, Microscopy, Fluorescence