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Image Search Results
Journal: eLife
Article Title: Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules
doi: 10.7554/elife.33864
Figure Lengend Snippet: Figure 1. The centrosome is not essential for angiogenic migration and sprouting. (A) Imaging of control or centrinone-treated HUVECs stained for MT (a-tubulin, cyan hot) using STED microscopy. Arrow points toward the centrosome and the plot shows the average fluorescence intensity of a-tubulin, n = 25 cells for each condition. (B,C) Analysis of MT plus ends in control or centrinone-treated HUVECs illustrated by maximum intensity projections and kymographs of EB3-GFP live fluorescence imaging (B) and EB3 staining (C). Plots show MT growth rate and catastrophe frequency, n = 96 tracks in Figure 1 continued on next page
Article Snippet: We used rabbit polyclonal antibodies against CAMSAP2 (Novus, Littleton, CO, NBP1-21402), CEP135, acetylated tubulin, polyglutamylated tubulin and g-tubulin (Sigma-Aldrich, St Louis, MO, SAB4503685, T7451, T9822 and T3559), CDK5RAP2 (BethylLaboratories, Montgomery, TX, A300-554A), detyrosinated tubulin (Abcam, UK, ab48389),
Techniques: Migration, Imaging, Control, Staining, Microscopy, Fluorescence