Journal: Nature communications

Article Title: Generation of colonic IgA-secreting cells in the caecal patch.

doi: 10.1038/ncomms4704

Figure Lengend Snippet: Figure 2 | Decreased IgA-secreting cells in large intestinal lamina propria of appendectomized germ-free mice after conventionalization. (a,b) Lamina propria lymphocytes were isolated from appendectomized or sham-operated germ-free(GF) mice at 4 weeks after conventionalization, and stained for surface IgA/B220 or intracellular IL-17/IFN-g/IL-10 in CD4 þ population. (a) Total numbers of IgA þ B220 cells as well as IL-17-, IFN-g- and IL-10-positive CD4 þ cells in the large (upper) and the small intestine (lower) are shown. Each symbol represents an individual mouse, and horizontal bars indicate the mean. (b) Representative dot plots for cells stained with anti-IgA and B220 of appendectomized and sham-operated mice. Numbers within plots indicate percentages of cells in respective areas. (c) Distribution of IgA þ cells in the small and large intestines. Frozen sections were stained with anti-IgA mAb (green) and DAPI (blue). Scale bars, 100 mm. (d) Faeces and sera were collected from appendectomized (n ¼ 12) or sham-operated mice (n ¼ 12) at 4 weeks after conventionalization and GF mice (n ¼ 12), and IgA levels of faeces and sera were determined by ELISA. Each symbol represents an individual mouse, and horizontal bars indicate the mean. Statistical analyses were performed with the Student’s t-test. *Po0.05. NS, not significant.

Article Snippet: IgA levels in the faeces, sera and culture supernatants were determined by a mouse IgA ELISA quantitation kit (Bethyl Laboratories).

Techniques: Isolation, Staining, Enzyme-linked Immunosorbent Assay

Journal: Microbial Biotechnology

Article Title: Polysaccharides L900/2 and L900/3 isolated from Lactobacillus rhamnosus LOCK 0900 modulate allergic sensitization to ovalbumin in a mouse model

doi: 10.1111/1751-7915.12606

Figure Lengend Snippet: Levels of OVA ‐specific IgE (A), IgG2a (B), IgG1 (C) and IgA (D) antibodies in serum, as measured by ELISA . Pooled values of two repeat experiments, each with four to six mice per group, are expressed as means ± SEM ; * P < 0.05, *** P < 0.001, **** P < 0.0001.

Article Snippet: The serum levels of total IgA and IgE were measured using a mouse IgA and IgE ELISA quantification kit (Bethyl, Montgomery, TX, USA) according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Microbial Biotechnology

Article Title: Polysaccharides L900/2 and L900/3 isolated from Lactobacillus rhamnosus LOCK 0900 modulate allergic sensitization to ovalbumin in a mouse model

doi: 10.1111/1751-7915.12606

Figure Lengend Snippet: Levels of total IgE (A) and IgA (B) in serum, as measured by ELISA . Pooled values of two repeat experiments, each with four to six mice per group.

Article Snippet: The serum levels of total IgA and IgE were measured using a mouse IgA and IgE ELISA quantification kit (Bethyl, Montgomery, TX, USA) according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Experimental & Molecular Medicine

Article Title: Obesity aggravates the joint inflammation in a collagen-induced arthritis model through deviation to Th17 differentiation

doi: 10.3858/emm.2012.44.7.047

Figure Lengend Snippet: The antigen-specific autoantibodies increased in the obese CIA mice. Ten weeks after the second immunization, sera from the obese CIA and CIA mice ( n = 6 for each group) were collected. The CII-specific total IgG (A) and IgG2a (B) concentrations were determined in the serum samples of the individual mice by ELISA. The data is expressed as the mean ± SD. * P < 0.01 compared with the CIA mice.

Article Snippet: The concentrations of IgG2a and IgG1 were measured using mouse IgG2a and IgG1 ELISA Quantitation Kits (Bethyl Laboratories, Montgomery, TX), respectively.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The lupus susceptibility locus Sle1 facilitates the peripheral development and selection of anti-DNA B cells through impaired receptor editing.

doi: 10.4049/jimmunol.1201558

Figure Lengend Snippet: FIGURE 1. Serum levels of IgM and IgG2a Abs to nuclear Ags in B6.56R and B6.Sle1.56R mice. Sera from 9–12-mo-old female B6.56R mice (s, n = 11) and B6.Sle1.56R mice (d, n = 12) were assayed for levels of IgM or IgG anti-ssDNA and anti-dsDNA Abs using ELISA. Each symbol represents a single mouse. Horizontal lines indicate the mean Ab levels in each strain. Mouse IgM and IgG Abs were used as standard control. The p values were obtained using Student t test comparison of B6.Sle1.56R data with the corresponding B6.56R data. The “a” denotes allotype originated from 56R VH chain (backcrossed with BALB/c).

Article Snippet: Mouse IgM and IgG ELISA Quantitative Kits (Bethyl Laboratories, Montgomery, TX) were used as a standard control.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Comparison