Journal: The Journal of Biological Chemistry

Article Title: An E2-guided E3 Screen Identifies the RNF17-UBE2U Pair as Regulator of the Radiosensitivity, Immunodeficiency, Dysmorphic Features, and Learning Difficulties (RIDDLE) Syndrome Protein RNF168 *

doi: 10.1074/jbc.M116.758854

Figure Lengend Snippet: Sequences of siRNAs

Article Snippet: The siRNA libraries targeting 37 E2s and 64 UBE2U-interacting E3s were purchased from GenePharma ( ). siRNAs for cell depletion of UBC13, RNF8, and RNF168 were described previously ( 31 , 56 ).

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: An E2-guided E3 Screen Identifies the RNF17-UBE2U Pair as Regulator of the Radiosensitivity, Immunodeficiency, Dysmorphic Features, and Learning Difficulties (RIDDLE) Syndrome Protein RNF168 *

doi: 10.1074/jbc.M116.758854

Figure Lengend Snippet: Screen of UBE2U-interacting E3s identifies novel regulators of DSB responses. A, network illustration of interactions between UBE2U and its potential E3 partners (35, 36). B and C, U2OS cells transfected with an E3-targeting siRNA library or the indicated siRNAs were irradiated (10 Gy). 4 h post-IR treatment, cells were fixed, permeabilized, and immunostained with antibodies against 53BP1 and γH2AX, and the percentage of 53BP1 focus-positive cells (>10 foci per cell) was quantified. Ranking by z score of all samples was plotted as described under “Experimental Procedures.” Representative images following RNAi-mediated depletion of RNF17, TRIM34, TRIM65, or RNF10 are shown (C). Non-targeting siRNAs (siCTR) or RNF168-targeting siRNAs (siRNF168) were used as negative and positive controls, respectively.

Article Snippet: The siRNA libraries targeting 37 E2s and 64 UBE2U-interacting E3s were purchased from GenePharma ( ). siRNAs for cell depletion of UBC13, RNF8, and RNF168 were described previously ( 31 , 56 ).

Techniques: Transfection, Irradiation

Journal: PLoS ONE

Article Title: Applications of minimally invasive multimodal telemetry for continuous monitoring of brain function and intracranial pressure in macaques with acute viral encephalitis

doi: 10.1371/journal.pone.0232381

Figure Lengend Snippet: Materials list.

Article Snippet: , E2S-1 Module , Data Sciences International , 275-0248-001 , Communicates between Ethernet switch and APR-1.

Techniques: Battery, Software