Journal: Cold Spring Harbor symposia on quantitative biology

Article Title: Regulation of the Arf/p53 tumor surveillance network by E2F.

doi: 10.1101/sqb.2005.70.050

Figure Lengend Snippet: Figure 5. The activating E2Fs bind to the Arf promoter in re- sponse to oncogenic stress. Wild-type MEFs were infected with retrovirus overexpressing E1A, or an empty virus (vector) and subjected to western (A) or ChIP (B) analyses with the indicated antisera. MEFs expressing E1A exhibit dramatically increased levels of p19Arf, coincident with recruitment of activating E2Fs (E2F1, E2F2, and E2F3A) to the Arf promoter.

Article Snippet: Sonicated, cross-linked chromatin corresponding to approximately 3 x 106 cells was immunoprecipitated with the following antibodies: normal rabbit IgG (control), sc-2027; E2F1, sc-193; E2F2, sc-633x; E2F3A, sc-879x; E2F3A+B, sc-878x; E2F4, sc-1082x; p130, sc-317x (all from Santa Cruz Biotechnology).

Techniques: Infection, Virus, Plasmid Preparation, Western Blot, Expressing

Journal: PLoS ONE

Article Title: Exploring Genetic Factors Involved in Huntington Disease Age of Onset: E2F2 as a New Potential Modifier Gene

doi: 10.1371/journal.pone.0131573

Figure Lengend Snippet: Multiple linear regression analysis between SNP genotypes and eAO.

Article Snippet: For Taqman-based assays, Hs00918089_m1 probe, which hybridizes with the 3–4 exon boundary of the E2F2 gene, was used.

Techniques:

Journal: PLoS ONE

Article Title: Exploring Genetic Factors Involved in Huntington Disease Age of Onset: E2F2 as a New Potential Modifier Gene

doi: 10.1371/journal.pone.0131573

Figure Lengend Snippet: Multiple linear regression analysis between SNP genotypes and mAO.

Article Snippet: For Taqman-based assays, Hs00918089_m1 probe, which hybridizes with the 3–4 exon boundary of the E2F2 gene, was used.

Techniques:

Journal: PLoS ONE

Article Title: Exploring Genetic Factors Involved in Huntington Disease Age of Onset: E2F2 as a New Potential Modifier Gene

doi: 10.1371/journal.pone.0131573

Figure Lengend Snippet: Two methods were used. In Taqman assay, the expression of E2F2 gene was analyzed in 31 samples (N TT = 4; N GT = 12, N GG = 15) with Hs00918089_m1 Taqman probe; the expression values were normalized respect to expression of B2M and YWHAZ reference genes. In SYBR Green assay, the E2F2 gene expression was estimated in 31 samples (N TT = 5; N GT = 14, N GG = 12); the expression values were normalized to expression of UBC and YWHAZ reference genes. Results are expressed as fold over respective GG individuals. Asterisk denotes statistically significant differences (P<0.05) between GG and any other group, according to DataAssist software analysis (T-test) or REST software analysis (Pair Wise Reallocation Randomization test).

Article Snippet: For Taqman-based assays, Hs00918089_m1 probe, which hybridizes with the 3–4 exon boundary of the E2F2 gene, was used.

Techniques: TaqMan Assay, Expressing, SYBR Green Assay, Gene Expression, Software