Journal: Shock (Augusta, Ga.)

Article Title: Evodiamine represses hypoxia-induced inflammatory proteins expression and hypoxia-inducible factor 1alpha accumulation in RAW264.7.

doi: 10.1097/SHK.0b013e31819940cb

Figure Lengend Snippet: FIG. 1. Effect of evodiamine on hypoxia-induced inflammatory response. RAW264.7 cells were incubated in the presence or absence of indicated concentration of evodiamine for 1 h and then incubated in normoxia (21% O2) or hypoxia (1% O2) for 24 h. A, Equal amount of total proteins was subjected to sodium dodecyl sulfateYpolyacrylamide gel electrophoresis to detect COX-2, iNOS, and actin expressions by Western blotting analysis. B, The culture medium was assayed by PGE2 ELISA kit. C, Cellular viability was assayed by SRB assay. N indicates normoxia; H, hypoxia, respectively. ***P G 0.001 compared with normoxic cells, and ##P G 0.01 compared with hypoxic cells. The representative result of three separate experiments is shown.

Article Snippet: Prostaglandin E2 (PGE2) assay kit was from R&D Systems (Minneapolis, Minn).

Techniques: Incubation, Concentration Assay, Nucleic Acid Electrophoresis, Western Blot, Enzyme-linked Immunosorbent Assay, Sulforhodamine B Assay