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mouse interferon β  (Cusabio)
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Cusabio mouse interferon β
( A ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1) for indicated time points, and then cell lysates were analyzed for EMCV replication level by qPCR. n = 3 biological replicates. Statistical significance was determined by two-tailed unpaired Student’s t-test. **** P = 0.000039 (4 h), *** P = 0.00050 (8 h), **** P = 0.000040 (12 h). ( B ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1) for indicated time points. The cell supernatant was harvested and analyzed by TCID 50 assay. n = 3 biological replicates. Statistical significance was determined by two-tailed unpaired Student’s t-test. ** P = 0.00540 (8 h), ** P = 0.00820 (12 h). ( C ) Western blot analysis of the indicated signaling proteins in MEFs from Rnf144b +/+ or Rnf144b −/− mice infected with EMCV (MOI = 1) for the indicated time periods. ( D ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1). After 12 h, cell lysates were analyzed for Ifnb1, TNF-α, IL-6 mRNA level by qPCR. n = 3 biological replicates. Statistical significance was determined by two-tailed unpaired Student’s t-test. **** P = 0.000009 (Ifnb1), *** P = 0.00090 (TNF-α), *** P = 0.00030 (IL-6). ( E ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1). 12 h post-infection, cells were treated by CHX (30 μM) for indicated time points. Protein extracts were used for immunoblot analysis of the endogenous MDA5 protein level. ( F , G ) Rnf144b +/+ and Rnf144b −/ − mice were intraperitoneally injected EMCV (5 × 10 6 PFU) for 48 h ( n = 5 per group). qPCR analysis of EMCV replication level in heart and brain, and mRNA level of Ifnb1, TNF-α, IL-6 in brain ( G ). Statistical significance was determined by two-tailed unpaired Student’s t-test. ** P = 0.00530 (heart), * P = 0.02620 (brain), *** P = 0.00030 (Ifnb1), ** P = 0.00170 (TNF-α), *** P = 0.00030 (IL-6). ( H ) ELISA of <t>IFN-β</t> production in serum of Rnf144b +/+ and Rnf144b −/− mice that were intraperitoneally injected with EMCV (5 × 10 6 PFU) for 12 h ( n = 3 per group). Statistical significance was determined by two-tailed unpaired Student’s t-test. **** P = 0.000020. ( I ) Survival of Rnf144b +/+ and Rnf144b −/− mice ( n = 20 per group) intraperitoneally infected with EMCV (6 × 10 7 PFU). Significance was tested using log-rank (Mantel-Cox) test, *** P = 0.00030. Data information: Data shown are representative of at least three biological replicates, with each data point representing a biological experiment. Error bars are presented as mean ± SD. Statistical significance was determined by Student’s t-test. .
Mouse Interferon β, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse interferon β - by Bioz Stars, 2026-08
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( A ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1) for indicated time points, and then cell lysates were analyzed for EMCV replication level by qPCR. n = 3 biological replicates. Statistical significance was determined by two-tailed unpaired Student’s t-test. **** P = 0.000039 (4 h), *** P = 0.00050 (8 h), **** P = 0.000040 (12 h). ( B ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1) for indicated time points. The cell supernatant was harvested and analyzed by TCID 50 assay. n = 3 biological replicates. Statistical significance was determined by two-tailed unpaired Student’s t-test. ** P = 0.00540 (8 h), ** P = 0.00820 (12 h). ( C ) Western blot analysis of the indicated signaling proteins in MEFs from Rnf144b +/+ or Rnf144b −/− mice infected with EMCV (MOI = 1) for the indicated time periods. ( D ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1). After 12 h, cell lysates were analyzed for Ifnb1, TNF-α, IL-6 mRNA level by qPCR. n = 3 biological replicates. Statistical significance was determined by two-tailed unpaired Student’s t-test. **** P = 0.000009 (Ifnb1), *** P = 0.00090 (TNF-α), *** P = 0.00030 (IL-6). ( E ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1). 12 h post-infection, cells were treated by CHX (30 μM) for indicated time points. Protein extracts were used for immunoblot analysis of the endogenous MDA5 protein level. ( F , G ) Rnf144b +/+ and Rnf144b −/ − mice were intraperitoneally injected EMCV (5 × 10 6 PFU) for 48 h ( n = 5 per group). qPCR analysis of EMCV replication level in heart and brain, and mRNA level of Ifnb1, TNF-α, IL-6 in brain ( G ). Statistical significance was determined by two-tailed unpaired Student’s t-test. ** P = 0.00530 (heart), * P = 0.02620 (brain), *** P = 0.00030 (Ifnb1), ** P = 0.00170 (TNF-α), *** P = 0.00030 (IL-6). ( H ) ELISA of IFN-β production in serum of Rnf144b +/+ and Rnf144b −/− mice that were intraperitoneally injected with EMCV (5 × 10 6 PFU) for 12 h ( n = 3 per group). Statistical significance was determined by two-tailed unpaired Student’s t-test. **** P = 0.000020. ( I ) Survival of Rnf144b +/+ and Rnf144b −/− mice ( n = 20 per group) intraperitoneally infected with EMCV (6 × 10 7 PFU). Significance was tested using log-rank (Mantel-Cox) test, *** P = 0.00030. Data information: Data shown are representative of at least three biological replicates, with each data point representing a biological experiment. Error bars are presented as mean ± SD. Statistical significance was determined by Student’s t-test. .

Journal: EMBO Reports

Article Title: RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation

doi: 10.1038/s44319-024-00256-w

Figure Lengend Snippet: ( A ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1) for indicated time points, and then cell lysates were analyzed for EMCV replication level by qPCR. n = 3 biological replicates. Statistical significance was determined by two-tailed unpaired Student’s t-test. **** P = 0.000039 (4 h), *** P = 0.00050 (8 h), **** P = 0.000040 (12 h). ( B ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1) for indicated time points. The cell supernatant was harvested and analyzed by TCID 50 assay. n = 3 biological replicates. Statistical significance was determined by two-tailed unpaired Student’s t-test. ** P = 0.00540 (8 h), ** P = 0.00820 (12 h). ( C ) Western blot analysis of the indicated signaling proteins in MEFs from Rnf144b +/+ or Rnf144b −/− mice infected with EMCV (MOI = 1) for the indicated time periods. ( D ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1). After 12 h, cell lysates were analyzed for Ifnb1, TNF-α, IL-6 mRNA level by qPCR. n = 3 biological replicates. Statistical significance was determined by two-tailed unpaired Student’s t-test. **** P = 0.000009 (Ifnb1), *** P = 0.00090 (TNF-α), *** P = 0.00030 (IL-6). ( E ) Rnf144b +/+ and Rnf144b −/− MEFs were infected with EMCV (MOI = 1). 12 h post-infection, cells were treated by CHX (30 μM) for indicated time points. Protein extracts were used for immunoblot analysis of the endogenous MDA5 protein level. ( F , G ) Rnf144b +/+ and Rnf144b −/ − mice were intraperitoneally injected EMCV (5 × 10 6 PFU) for 48 h ( n = 5 per group). qPCR analysis of EMCV replication level in heart and brain, and mRNA level of Ifnb1, TNF-α, IL-6 in brain ( G ). Statistical significance was determined by two-tailed unpaired Student’s t-test. ** P = 0.00530 (heart), * P = 0.02620 (brain), *** P = 0.00030 (Ifnb1), ** P = 0.00170 (TNF-α), *** P = 0.00030 (IL-6). ( H ) ELISA of IFN-β production in serum of Rnf144b +/+ and Rnf144b −/− mice that were intraperitoneally injected with EMCV (5 × 10 6 PFU) for 12 h ( n = 3 per group). Statistical significance was determined by two-tailed unpaired Student’s t-test. **** P = 0.000020. ( I ) Survival of Rnf144b +/+ and Rnf144b −/− mice ( n = 20 per group) intraperitoneally infected with EMCV (6 × 10 7 PFU). Significance was tested using log-rank (Mantel-Cox) test, *** P = 0.00030. Data information: Data shown are representative of at least three biological replicates, with each data point representing a biological experiment. Error bars are presented as mean ± SD. Statistical significance was determined by Student’s t-test. .

Article Snippet: Mouse Interferon β (IFN-β/IFNB) ELISA Kit , CUSABIO , Cat#E04945m.

Techniques: Infection, Two Tailed Test, Western Blot, Injection, Enzyme-linked Immunosorbent Assay

Reagents and tools table

Journal: EMBO Reports

Article Title: RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation

doi: 10.1038/s44319-024-00256-w

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Mouse Interferon β (IFN-β/IFNB) ELISA Kit , CUSABIO , Cat#E04945m.

Techniques: Recombinant, Sequencing, shRNA, Mutagenesis, RNA Extraction, Plasmid Preparation, Enzyme-linked Immunosorbent Assay