Journal: The Journal of Neuroscience

Article Title: Gap Junctions Mediate Bystander Cell Death in Developing Retina

doi: 10.1523/JNEUROSCI.23-16-06413.2003

Figure Lengend Snippet: Carbenoxolone decreased dye coupling in the scrape-loading model. Shown is control P13 rat retina scrape-loaded with RD (a) and Neurobiotin (b), which was visualized with Cy2-streptavidin. In a and b note that many Neurobiotin-labeled cells were not labeled with RD (arrowheads), indicating that they were coupled to the scrape-loaded cells (arrows). c, d, P13 retina treated with carbenoxolone (20 mg/kg, s.c., followed by 10 μm), scrape-loaded with RD and Neurobiotin. d, Neurobiotin labeling of same field as in c; only occasional cells were single labeled for Neurobiotin (arrowheads), indicating decreased coupling as compared with control (a, b). The same concentration of carbenoxolone that decreased bystander cell killing inhibited coupling to similar levels. Scale bars in a-d, 20 μm. e, The Coupling Index for retinas treated with carbenoxolone demonstrates that this gap junction inhibitor decreased the Coupling Index significantly as compared with control (asterisk indicates group significantly different from control; two-tailed t test, p < 0.01). Con, Control; Cbx, carbenoxolone.

Article Snippet: All liquid was removed, and 10 μl of 0.5% rhodamine dextran (RD; Molecular Probes, Eugene, OR) and 3% Neurobiotin (NB; Vector Laboratories, Burlingame, CA) solution in 100 m m KCl was placed on top of the retina.

Techniques: Labeling, Concentration Assay, Two Tailed Test

Journal: Endocrinology

Article Title: Adiponectin Exerts Neurotrophic Effects on Dendritic Arborization, Spinogenesis, and Neurogenesis of the Dentate Gyrus of Male Mice

doi: 10.1210/en.2015-2078

Figure Lengend Snippet: Antibody Table

Article Snippet: Next day, the sections were coincubated with the following Alexa Fluor-conjugated secondary antibodies for 4 hours: antirabbit Alexa Fluor 647 goat IgG, antimouse Alexa Fluor 594 goat IgG, and antirat Alexa Fluor 488 goal IgG (1:400; Life Technologies) ( ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Peptide/Protein Target Antigen Sequence (if Known) Name of Antibody Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody Species Raised in; Monoclonal or Polyclonal Dilution Used Ki67 Synthetic peptide conjugated to KLH derived from within residues 1200 − 1300 of Human Ki67 Anti-Ki67 antibody Abcam, Ab15580 Rabbit; polyclonal 1:1000 Doublecortin (DCX) epitope mapping at the C-terminus of Doublecortin of human origin Doublecortin antibody (C-18) Santa Cruz Biotechnology, Inc, sc-8066 Goat; polyclonal 1:200 BrdU Anti-BrdU antibody [BU1/75 (ICR1)] Accurate Chemical, YSRTMCA2060GA Rat; monoclonal 1:400 NeuN Anti-NeuN antibody, clone A60 Millipore, MAB377 Mouse; monoclonal 1:1000 GFAP Anti-glial fibrillary acidic protein (GFAP) antibody Millipore, AB5804 Rabbit; polyclonal 1:1000 Biotinylated antirabbit IgG Biotinylated goat antirabbit IgG antibody Vector Laboratories, BA-1000 Goat 1:400 Biotinylated antigoat IgG Biotinylated horse antigoat IgG antibody Vector Laboratories, BA-9500 Horse 1:400 Alexa Fluor 488 goat antirat IgG Goat antirat IgG (H + L) secondary antibody, Alexa Fluor 488 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A11006","term_id":"492389","term_text":"A11006"}} A11006 Goat 1:400 Alexa Fluor 546 goat antimouse IgG F(ab')2-goat antimouse IgG (H + L) secondary antibody, Alexa Fluor 546 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A11018","term_id":"492393","term_text":"A11018"}} A11018 Goat 1:400 Alexa Fluor 647 goat antirabbit IgG F(ab')2-goat antirabbit IgG (H + L) secondary antibody, Alexa Fluor 647 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A21246","term_id":"641368","term_text":"A21246"}} A21246 Goat 1:400 Open in a separate window Abbreviation: H+L, Heavy and light chain.

Techniques: Sequencing, Derivative Assay, Plasmid Preparation

Journal: Journal of Biological Chemistry

Article Title: Disrupted glucose homeostasis and skeletal-muscle-specific glucose uptake in an exocyst knockout mouse model

doi: 10.1016/j.jbc.2021.100482

Figure Lengend Snippet: Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.

Article Snippet: Chem. (2021) 296 100482 After permeabilization and blocking, sections were incubated with primary antibodies (anti-insulin (Cat #66198-1-Ig, Proteintech Group, Inc); anti-glucagon (Cat # 15954-1- AP,Proteintech Group, Inc)) overnight at 4 C and incubated with appropriate fluorescent secondary antibodies (DyLight 488 Anti-Rabbit IgG and DyLight 594 Anti-Mouse, Vector Laboratories) for detection.

Techniques: Generated, Microscopy, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation

Journal: International journal of biological macromolecules

Article Title: Structural insights into acetylated histone ligand recognition by the BDP1 bromodomain of Plasmodium falciparum

doi: 10.1016/j.ijbiomac.2022.10.247

Figure Lengend Snippet: Structural analysis of the Pf BDP1-BRD (PDB ID: 7M97) compared to human bromodomains in families I–VIII.

Article Snippet: The codon-optimized Pf BDP1-BRD (303–488) construct cloned in a pGEX-6P-1 vector with an N terminal GST-tag followed by a precision protease site was obtained from GenScript.

Techniques: Sequencing, Binding Assay

Journal: International journal of biological macromolecules

Article Title: Structural insights into acetylated histone ligand recognition by the BDP1 bromodomain of Plasmodium falciparum

doi: 10.1016/j.ijbiomac.2022.10.247

Figure Lengend Snippet: Binding affinities of the Pf BDP1-BRD with acetylated histone peptides measured by ITC.

Article Snippet: The codon-optimized Pf BDP1-BRD (303–488) construct cloned in a pGEX-6P-1 vector with an N terminal GST-tag followed by a precision protease site was obtained from GenScript.

Techniques: Binding Assay, Sequencing