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gas6 expression  (R&D Systems)
96
R&D Systems gas6 expression
( A ) mRNA expression in control and <t>Gas6</t> overexpressed PCa cells. mRNA was extracted 2 days after serum starvation. *p < 0.05, **p < 0.01 compared to PC3 Control cells. ## p < 0.01 compared to DU145 Control cells. ( B ) mRNA expression in sh control and sh Gas6 knockdown PCa cells. mRNA was extracted after 6 days of culture in 0.5% FBS. Culture media was replaced at day3.*p < 0.05, **p < 0.01 compared to PC3 sh Control cells. ## p < 0.01 compared to DU145 sh Control cells. ( C–E ) mRNA expression in PCa cells 6 days after siRNA treatments targeting Axl. *p < 0.05, **p < 0.01 compared to si scramble control PC3cells, ## p < 0.01 compared to si scramble control DU145 cells. ( F,G ) Western blots showing protein expression levels of Axl, TGFBR2 and TGFBBR3 in PCa cells after the reduction of Axl or Gas6 by shRNA ( F ) or siRNA ( G ). Protein samples were loaded 6 days after siRNA treatments targeting Axl (G) .
Gas6 Expression, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gas6 expression/product/R&D Systems
Average 96 stars, based on 1 article reviews
gas6 expression - by Bioz Stars, 2026-04
96/100 stars
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dy986  (R&D Systems)
93
R&D Systems dy986
( A ) mRNA expression in control and <t>Gas6</t> overexpressed PCa cells. mRNA was extracted 2 days after serum starvation. *p < 0.05, **p < 0.01 compared to PC3 Control cells. ## p < 0.01 compared to DU145 Control cells. ( B ) mRNA expression in sh control and sh Gas6 knockdown PCa cells. mRNA was extracted after 6 days of culture in 0.5% FBS. Culture media was replaced at day3.*p < 0.05, **p < 0.01 compared to PC3 sh Control cells. ## p < 0.01 compared to DU145 sh Control cells. ( C–E ) mRNA expression in PCa cells 6 days after siRNA treatments targeting Axl. *p < 0.05, **p < 0.01 compared to si scramble control PC3cells, ## p < 0.01 compared to si scramble control DU145 cells. ( F,G ) Western blots showing protein expression levels of Axl, TGFBR2 and TGFBBR3 in PCa cells after the reduction of Axl or Gas6 by shRNA ( F ) or siRNA ( G ). Protein samples were loaded 6 days after siRNA treatments targeting Axl (G) .
Dy986, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dy986/product/R&D Systems
Average 93 stars, based on 1 article reviews
dy986 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier

Image Search Results


( A ) mRNA expression in control and Gas6 overexpressed PCa cells. mRNA was extracted 2 days after serum starvation. *p < 0.05, **p < 0.01 compared to PC3 Control cells. ## p < 0.01 compared to DU145 Control cells. ( B ) mRNA expression in sh control and sh Gas6 knockdown PCa cells. mRNA was extracted after 6 days of culture in 0.5% FBS. Culture media was replaced at day3.*p < 0.05, **p < 0.01 compared to PC3 sh Control cells. ## p < 0.01 compared to DU145 sh Control cells. ( C–E ) mRNA expression in PCa cells 6 days after siRNA treatments targeting Axl. *p < 0.05, **p < 0.01 compared to si scramble control PC3cells, ## p < 0.01 compared to si scramble control DU145 cells. ( F,G ) Western blots showing protein expression levels of Axl, TGFBR2 and TGFBBR3 in PCa cells after the reduction of Axl or Gas6 by shRNA ( F ) or siRNA ( G ). Protein samples were loaded 6 days after siRNA treatments targeting Axl (G) .

Journal: Scientific Reports

Article Title: Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow

doi: 10.1038/srep36520

Figure Lengend Snippet: ( A ) mRNA expression in control and Gas6 overexpressed PCa cells. mRNA was extracted 2 days after serum starvation. *p < 0.05, **p < 0.01 compared to PC3 Control cells. ## p < 0.01 compared to DU145 Control cells. ( B ) mRNA expression in sh control and sh Gas6 knockdown PCa cells. mRNA was extracted after 6 days of culture in 0.5% FBS. Culture media was replaced at day3.*p < 0.05, **p < 0.01 compared to PC3 sh Control cells. ## p < 0.01 compared to DU145 sh Control cells. ( C–E ) mRNA expression in PCa cells 6 days after siRNA treatments targeting Axl. *p < 0.05, **p < 0.01 compared to si scramble control PC3cells, ## p < 0.01 compared to si scramble control DU145 cells. ( F,G ) Western blots showing protein expression levels of Axl, TGFBR2 and TGFBBR3 in PCa cells after the reduction of Axl or Gas6 by shRNA ( F ) or siRNA ( G ). Protein samples were loaded 6 days after siRNA treatments targeting Axl (G) .

Article Snippet: An antibody sandwich ELISA was used to evaluate Gas6 expression in the conditioned media and cell lysates by the following the directions of manufacturer (Cat. #DY986 and DY885, R&D Systems, Minneapolis, MN).

Techniques: Expressing, Control, Knockdown, Western Blot, shRNA

( A ) Relative mRNA levels in PCa cells were evaluated 24 hours after TGF-β1 (Cat #: 240-B/CF, R&D Systems, Minneapolis, MN) or TGF-β2 (Cat #: 302-B2/CF, R&D Systems, Minneapolis, MN) stimulation (both 2 ng/ml). *p < 0.05, **p < 0.01 compared to PCa cells without TGF-β stimulation, # p < 0.05, ## p < 0.01 compared to PCa with TGF-β1 stimulation. ( B,C ) PCa cells were pre-labeled with DiD, and DiD retention was evaluated 6 days after TGF-β2 stimulation by flow cytometry. *p < 0.05, **p < 0.01 compared to PC3 cells without TGF-β2 treatments, ## p < 0.01 compared to DU145 cells without TGF-β2 treatments in each of sh Control, sh Axl or sh Gas6 cells. ( D ) Western blots showing p27 expression levels in PCa cells 3 days after TGF-β2 treatment. ( E ) Relative p27 expression levels evaluated by densitometry. P27 expression was normalized by β-actin expression. *p < 0.05, **p < 0.01 compared to PCa sh Control cells without TGF-β2 treatments. # p < 0.05, ## p < 0.01 compared to DU145 sh Axl cells without TGF-β2 stimulation.

Journal: Scientific Reports

Article Title: Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow

doi: 10.1038/srep36520

Figure Lengend Snippet: ( A ) Relative mRNA levels in PCa cells were evaluated 24 hours after TGF-β1 (Cat #: 240-B/CF, R&D Systems, Minneapolis, MN) or TGF-β2 (Cat #: 302-B2/CF, R&D Systems, Minneapolis, MN) stimulation (both 2 ng/ml). *p < 0.05, **p < 0.01 compared to PCa cells without TGF-β stimulation, # p < 0.05, ## p < 0.01 compared to PCa with TGF-β1 stimulation. ( B,C ) PCa cells were pre-labeled with DiD, and DiD retention was evaluated 6 days after TGF-β2 stimulation by flow cytometry. *p < 0.05, **p < 0.01 compared to PC3 cells without TGF-β2 treatments, ## p < 0.01 compared to DU145 cells without TGF-β2 treatments in each of sh Control, sh Axl or sh Gas6 cells. ( D ) Western blots showing p27 expression levels in PCa cells 3 days after TGF-β2 treatment. ( E ) Relative p27 expression levels evaluated by densitometry. P27 expression was normalized by β-actin expression. *p < 0.05, **p < 0.01 compared to PCa sh Control cells without TGF-β2 treatments. # p < 0.05, ## p < 0.01 compared to DU145 sh Axl cells without TGF-β2 stimulation.

Article Snippet: An antibody sandwich ELISA was used to evaluate Gas6 expression in the conditioned media and cell lysates by the following the directions of manufacturer (Cat. #DY986 and DY885, R&D Systems, Minneapolis, MN).

Techniques: Labeling, Flow Cytometry, Control, Western Blot, Expressing

Gas6 produced by osteoblasts binds to Axl expressed by disseminated PCa cells, and its signaling induces expression of both TGF-β ligands (TGF-β1 and TGF-β2) and their receptors (TGFBR2 and TGFBR3). Subsequently, autocrine and paracrine TGF-β signaling induces PCa dormancy.

Journal: Scientific Reports

Article Title: Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow

doi: 10.1038/srep36520

Figure Lengend Snippet: Gas6 produced by osteoblasts binds to Axl expressed by disseminated PCa cells, and its signaling induces expression of both TGF-β ligands (TGF-β1 and TGF-β2) and their receptors (TGFBR2 and TGFBR3). Subsequently, autocrine and paracrine TGF-β signaling induces PCa dormancy.

Article Snippet: An antibody sandwich ELISA was used to evaluate Gas6 expression in the conditioned media and cell lysates by the following the directions of manufacturer (Cat. #DY986 and DY885, R&D Systems, Minneapolis, MN).

Techniques: Produced, Expressing