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duoset human pdgf aa elisa kit  (R&D Systems)
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R&D Systems duoset human pdgf aa elisa kit
The DEGs were further validated by RT-qPCR and analysis of protein expression. Four differentially regulated genes per cell line are depicted. Gene expression was normalized to ACTB (housekeeping gene) and the corresponding control vector cells (2 −ΔΔct method). A qPCR analysis of four representative DEGs from HCT-116 5-LO KO cells. B Secretion of TGFβ 2 into the cell culture supernatants of HCT-116 5-LO KO cells measured via <t>ELISA.</t> Depicted are the relative TGFβ 2 amounts in % compared to the vector control (mean TGFβ 2 secretion from vector control cells: 231.9 ± 140 pg/µg total protein). C qPCR analysis of four representative DEGs from HT-29 5-LO KO cells and D U-2 OS cells. E Cytokine release from U-2 OS 5-LO KO cells. Data are depicted as relative cytokine amounts in % compared to the corresponding control vector cells. PDGF-AA was assessed by ELISA while fractalkine ( CX3CL1 ) and MCP-1 ( CCL2 ) were measured via FACS employing a cytometric bead array (mean secretion from vector control cells: fractalkine, 64.9 ± 9.2 pg/µg protein; MCP-1, 978.2 ± 101.8 pg/µg protein; PDGF-AA, 255,6 ± 95.6 pg/µg protein). The complete mRNA expression data can be found in supplementary Fig. and supplementary table . All data are presented as mean + SD of three independent experiments. Asterisks indicate significant changes vs. control vector cells. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
Duoset Human Pdgf Aa Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dy221  (R&D Systems)
93
R&D Systems dy221
The DEGs were further validated by RT-qPCR and analysis of protein expression. Four differentially regulated genes per cell line are depicted. Gene expression was normalized to ACTB (housekeeping gene) and the corresponding control vector cells (2 −ΔΔct method). A qPCR analysis of four representative DEGs from HCT-116 5-LO KO cells. B Secretion of TGFβ 2 into the cell culture supernatants of HCT-116 5-LO KO cells measured via <t>ELISA.</t> Depicted are the relative TGFβ 2 amounts in % compared to the vector control (mean TGFβ 2 secretion from vector control cells: 231.9 ± 140 pg/µg total protein). C qPCR analysis of four representative DEGs from HT-29 5-LO KO cells and D U-2 OS cells. E Cytokine release from U-2 OS 5-LO KO cells. Data are depicted as relative cytokine amounts in % compared to the corresponding control vector cells. PDGF-AA was assessed by ELISA while fractalkine ( CX3CL1 ) and MCP-1 ( CCL2 ) were measured via FACS employing a cytometric bead array (mean secretion from vector control cells: fractalkine, 64.9 ± 9.2 pg/µg protein; MCP-1, 978.2 ± 101.8 pg/µg protein; PDGF-AA, 255,6 ± 95.6 pg/µg protein). The complete mRNA expression data can be found in supplementary Fig. and supplementary table . All data are presented as mean + SD of three independent experiments. Asterisks indicate significant changes vs. control vector cells. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
Dy221, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dy221/product/R&D Systems
Average 93 stars, based on 1 article reviews
dy221 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier

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The DEGs were further validated by RT-qPCR and analysis of protein expression. Four differentially regulated genes per cell line are depicted. Gene expression was normalized to ACTB (housekeeping gene) and the corresponding control vector cells (2 −ΔΔct method). A qPCR analysis of four representative DEGs from HCT-116 5-LO KO cells. B Secretion of TGFβ 2 into the cell culture supernatants of HCT-116 5-LO KO cells measured via ELISA. Depicted are the relative TGFβ 2 amounts in % compared to the vector control (mean TGFβ 2 secretion from vector control cells: 231.9 ± 140 pg/µg total protein). C qPCR analysis of four representative DEGs from HT-29 5-LO KO cells and D U-2 OS cells. E Cytokine release from U-2 OS 5-LO KO cells. Data are depicted as relative cytokine amounts in % compared to the corresponding control vector cells. PDGF-AA was assessed by ELISA while fractalkine ( CX3CL1 ) and MCP-1 ( CCL2 ) were measured via FACS employing a cytometric bead array (mean secretion from vector control cells: fractalkine, 64.9 ± 9.2 pg/µg protein; MCP-1, 978.2 ± 101.8 pg/µg protein; PDGF-AA, 255,6 ± 95.6 pg/µg protein). The complete mRNA expression data can be found in supplementary Fig. and supplementary table . All data are presented as mean + SD of three independent experiments. Asterisks indicate significant changes vs. control vector cells. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Journal: Cancer Gene Therapy

Article Title: Knock-out of 5-lipoxygenase in overexpressing tumor cells—consequences on gene expression and cellular function

doi: 10.1038/s41417-022-00531-9

Figure Lengend Snippet: The DEGs were further validated by RT-qPCR and analysis of protein expression. Four differentially regulated genes per cell line are depicted. Gene expression was normalized to ACTB (housekeeping gene) and the corresponding control vector cells (2 −ΔΔct method). A qPCR analysis of four representative DEGs from HCT-116 5-LO KO cells. B Secretion of TGFβ 2 into the cell culture supernatants of HCT-116 5-LO KO cells measured via ELISA. Depicted are the relative TGFβ 2 amounts in % compared to the vector control (mean TGFβ 2 secretion from vector control cells: 231.9 ± 140 pg/µg total protein). C qPCR analysis of four representative DEGs from HT-29 5-LO KO cells and D U-2 OS cells. E Cytokine release from U-2 OS 5-LO KO cells. Data are depicted as relative cytokine amounts in % compared to the corresponding control vector cells. PDGF-AA was assessed by ELISA while fractalkine ( CX3CL1 ) and MCP-1 ( CCL2 ) were measured via FACS employing a cytometric bead array (mean secretion from vector control cells: fractalkine, 64.9 ± 9.2 pg/µg protein; MCP-1, 978.2 ± 101.8 pg/µg protein; PDGF-AA, 255,6 ± 95.6 pg/µg protein). The complete mRNA expression data can be found in supplementary Fig. and supplementary table . All data are presented as mean + SD of three independent experiments. Asterisks indicate significant changes vs. control vector cells. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Article Snippet: PDGF-AA was measured with the DuoSet ® Human PDGF-AA ELISA Kit (R&D systems) following the manufacturer’s protocol.

Techniques: Quantitative RT-PCR, Expressing, Gene Expression, Control, Plasmid Preparation, Cell Culture, Enzyme-linked Immunosorbent Assay