Journal: Viruses

Article Title: Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

doi: 10.3390/v12060582

Figure Lengend Snippet: Specifications of the available polymerase chain reaction (PCR) commercial kits for laboratory diagnosis of COVID-19.

Article Snippet: AniCon Labor GmbH , Duplex Real-Time RT-PCR , Kylt ® SARS-CoV-2 Confirmation RT-qPCR , RdRP and S genes , 10 copies per μL of RNA , Detects all 92 available full genome sequences of SARS-CoV-2 (NCBI) , NR , CE-IVD.

Techniques: Polymerase Chain Reaction, Biomarker Discovery, Real-time Polymerase Chain Reaction, Multiplex Assay, Virus, Quantitative RT-PCR, Fluorescence, In Silico, Acid Assay, Detection Assay, Diagnostic Assay, Amplification, Reverse Transcription Polymerase Chain Reaction

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Comparison of infections with respiratory syncytial virus between children and adults: a multicenter surveillance from 2015 to 2019 in Beijing, China

doi: 10.1007/s10096-022-04492-7

Figure Lengend Snippet: RSV seasonality in Beijing . A The average percentage of RSV-positive cases per month among the tested cases in Beijing from March 2015 to February 2019. Gray bars denote the tested cases per month, blue bars denote the RSV-positive cases per month, and the brown line denotes the average percentage of RSV-positive cases per month among the tested cases. B RSV detection rate by month in Beijing from March 2015 to February 2019. The bars represent the RSV detection rate per month. Blue denotes RSV group A, orange denotes RSV group B, gray denotes untyped RSV. RSV, respiratory syncytial virus

Article Snippet: RSV-positive samples were further genotyped into RSV group A (RSV-A) or RSV group B (RSV-B) using a commercial kit (Duplex Real-Time PCR Kit for RSV, Beijing Kinghawk Pharmaceutical Co., Ltd., Beijing, China).

Techniques: Virus

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Comparison of infections with respiratory syncytial virus between children and adults: a multicenter surveillance from 2015 to 2019 in Beijing, China

doi: 10.1007/s10096-022-04492-7

Figure Lengend Snippet: Distribution of RSV infections by age group in Beijing from March 2015 to February 2019. A RSV detection rate by age group. The bar represents the RSV detection rate per age group. Blue denotes RSV group A, orange denotes RSV group B, and gray denotes untyped RSV. RSV, respiratory syncytial virus. B Proportion of RSV-positive cases by age group

Article Snippet: RSV-positive samples were further genotyped into RSV group A (RSV-A) or RSV group B (RSV-B) using a commercial kit (Duplex Real-Time PCR Kit for RSV, Beijing Kinghawk Pharmaceutical Co., Ltd., Beijing, China).

Techniques: Virus

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Comparison of infections with respiratory syncytial virus between children and adults: a multicenter surveillance from 2015 to 2019 in Beijing, China

doi: 10.1007/s10096-022-04492-7

Figure Lengend Snippet: The detection rates of RSV subgroups by age group and illness severity in Beijing from March 2015 to February 2019. The bar represents RSV detection rate per age group and illness severity. Blue denotes RSV group A, orange denotes RSV group B, and gray denotes untyped RSV. RSV, respiratory syncytial virus. AURTI, acute upper respiratory tract infection. NSCAP, non-severe community-acquired pneumonia. SCAP, severe community-acquired pneumonia

Article Snippet: RSV-positive samples were further genotyped into RSV group A (RSV-A) or RSV group B (RSV-B) using a commercial kit (Duplex Real-Time PCR Kit for RSV, Beijing Kinghawk Pharmaceutical Co., Ltd., Beijing, China).

Techniques: Virus, Infection

Journal: Virus Genes

Article Title: Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines

doi: 10.1007/s11262-012-0713-1

Figure Lengend Snippet: Published cases of PEDV outbreaks from several countries (1978–2011)

Article Snippet: Especially, endemic situation of PED infection brought the several commercialised PED virus detection systems using diagnosis techniques including conventional duplex RT-PCR (iNtRON Biotechnology, Inc, Korea), real time RT-PCR (kogenebiotech, Kore), DPO based multiplex RT-PCR (Seegene, Seoul, Korea), and immunochromatography (Bionote, Korea) in Korea.

Techniques: Virus, Infection, Isolation, Histopathology, Neutralization

Journal: Frontiers in Microbiology

Article Title: Evaluation of PCR Based Assays for the Improvement of Proportion Estimation of Bacterial and Viral Pathogens in Diarrheal Surveillance

doi: 10.3389/fmicb.2016.00386

Figure Lengend Snippet: Summary of the detection results in this study .

Article Snippet: V. cholerae and V. parahaemolyticus were detected from stool samples and APW broths using a V. cholerae / V. parahaemolyticus duplex real-time PCR kit (Huirui, Shanghai, China).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Evaluation of PCR Based Assays for the Improvement of Proportion Estimation of Bacterial and Viral Pathogens in Diarrheal Surveillance

doi: 10.3389/fmicb.2016.00386

Figure Lengend Snippet: Positive sample numbers and sensitivities of different methods for different pathogens .

Article Snippet: V. cholerae and V. parahaemolyticus were detected from stool samples and APW broths using a V. cholerae / V. parahaemolyticus duplex real-time PCR kit (Huirui, Shanghai, China).

Techniques:

Journal: Journal of Virological Methods

Article Title: Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus

doi: 10.1016/j.jviromet.2016.03.016

Figure Lengend Snippet: Primers and probes of various PEDV and PDCoV RT-PCRs evaluated in this study.

Article Snippet: Primers and probes of the duplex rRT-PCR for PEDV and PDCoV (IQ REAL PEDV/PDCoV Quantitative System, GeneReach USA) were designed to target two different highly conserved regions in the M gene among PEDV strains from all known clades and among all known PDCoV strains, respectively ( ).

Techniques: Sequencing, Amplification, Quantitative RT-PCR

Journal: Journal of Virological Methods

Article Title: Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus

doi: 10.1016/j.jviromet.2016.03.016

Figure Lengend Snippet: Specificity of various PEDV and PDCoV RT-PCRs evaluated in this study. (For interpretation of the references to color in this table legend, the reader is referred to the web version of this article.)

Article Snippet: Primers and probes of the duplex rRT-PCR for PEDV and PDCoV (IQ REAL PEDV/PDCoV Quantitative System, GeneReach USA) were designed to target two different highly conserved regions in the M gene among PEDV strains from all known clades and among all known PDCoV strains, respectively ( ).

Techniques:

Journal: Journal of Virological Methods

Article Title: Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus

doi: 10.1016/j.jviromet.2016.03.016

Figure Lengend Snippet: Analytical sensitivity of PEDV RT-iiPCR and duplex PEDV/PDCoV real-time RT-PCR for detection of PEDV RNA and comparison with the reference singleplex PEDV real-time RT-PCR using viral RNA from the serially diluted PEDV cell culture isolates. (For interpretation of the references to color in this table legend, the reader is referred to the web version of this article.)

Article Snippet: Primers and probes of the duplex rRT-PCR for PEDV and PDCoV (IQ REAL PEDV/PDCoV Quantitative System, GeneReach USA) were designed to target two different highly conserved regions in the M gene among PEDV strains from all known clades and among all known PDCoV strains, respectively ( ).

Techniques: Quantitative RT-PCR, Comparison, Cell Culture

Journal: Journal of Virological Methods

Article Title: Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus

doi: 10.1016/j.jviromet.2016.03.016

Figure Lengend Snippet: Analytical sensitivity of PDCoV RT-iiPCR and duplex PEDV/PDCoV real-time RT-PCR for detection of PDCoV RNA and comparison with the reference singleplex PDCoV real-time RT-PCR using viral RNA from the serially diluted PDCoV cell culture isolate. (For interpretation of the references to color in this table legend, the reader is referred to the web version of this article.)

Article Snippet: Primers and probes of the duplex rRT-PCR for PEDV and PDCoV (IQ REAL PEDV/PDCoV Quantitative System, GeneReach USA) were designed to target two different highly conserved regions in the M gene among PEDV strains from all known clades and among all known PDCoV strains, respectively ( ).

Techniques: Quantitative RT-PCR, Comparison, Cell Culture

Journal: Journal of Virological Methods

Article Title: Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus

doi: 10.1016/j.jviromet.2016.03.016

Figure Lengend Snippet: Specimen types of 170 clinical samples and Ct ranges of positive samples tested by the reference PEDV rRT-PCR and PDCoV rRT-PCR.

Article Snippet: Primers and probes of the duplex rRT-PCR for PEDV and PDCoV (IQ REAL PEDV/PDCoV Quantitative System, GeneReach USA) were designed to target two different highly conserved regions in the M gene among PEDV strains from all known clades and among all known PDCoV strains, respectively ( ).

Techniques:

Journal: Journal of Virological Methods

Article Title: Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus

doi: 10.1016/j.jviromet.2016.03.016

Figure Lengend Snippet: Sensitivity and specificity of PEDV RT-iiPCR, PDCoV RT-iiPCR, and duplex PEDV/PDCoV rRT-PCR as compared to the reference singleplex PEDV rRT-PCR and PDCoV rRT-PCR based on testing 170 clinical samples.

Article Snippet: Primers and probes of the duplex rRT-PCR for PEDV and PDCoV (IQ REAL PEDV/PDCoV Quantitative System, GeneReach USA) were designed to target two different highly conserved regions in the M gene among PEDV strains from all known clades and among all known PDCoV strains, respectively ( ).

Techniques:

Journal: Journal of Virological Methods

Article Title: Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus

doi: 10.1016/j.jviromet.2016.03.016

Figure Lengend Snippet: Discrepancies on clinical samples by various PEDV and PDCoV RT-PCRs. (For interpretation of the references to color in this table legend, the reader is referred to the web version of this article.)

Article Snippet: Primers and probes of the duplex rRT-PCR for PEDV and PDCoV (IQ REAL PEDV/PDCoV Quantitative System, GeneReach USA) were designed to target two different highly conserved regions in the M gene among PEDV strains from all known clades and among all known PDCoV strains, respectively ( ).

Techniques:

Journal: Journal of Virological Methods

Article Title: Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus

doi: 10.1016/j.jviromet.2016.03.016

Figure Lengend Snippet: Sensitivity and specificity of PEDV RT-iiPCR and PDCoV RT-iiPCR as compared to the reference singleplex PEDV rRT-PCR and PDCoV rRT-PCR based on testing 170 clinical samples with different nucleic acid extraction methods.

Article Snippet: Primers and probes of the duplex rRT-PCR for PEDV and PDCoV (IQ REAL PEDV/PDCoV Quantitative System, GeneReach USA) were designed to target two different highly conserved regions in the M gene among PEDV strains from all known clades and among all known PDCoV strains, respectively ( ).

Techniques: Extraction, Comparison