Journal: Pharmacological research

Article Title: A selective alpha1D-adrenoreceptor antagonist inhibits human prostate cancer cell proliferation and motility "in vitro".

doi: 10.1016/j.phrs.2015.11.017

Figure Lengend Snippet: Figure 4: Cell cycle analysis and p27 and p21 levels in PC3 and DU145 cells exposed to A175.

Article Snippet: Human androgen independent prostate cancer PC3 and DU145 cell lines and the androgendependent LNCaP cell line were originally obtained from American Type Culture Collection (ATCC, Rockville, MD, USA); CWR22Rv1 (Rv1) cells were originally obtained from LeibnitzInstitut DSMZ (Braunschweig, Germany).

Techniques: Cell Cycle Assay

Journal: Pharmacological research

Article Title: A selective alpha1D-adrenoreceptor antagonist inhibits human prostate cancer cell proliferation and motility "in vitro".

doi: 10.1016/j.phrs.2015.11.017

Figure Lengend Snippet: Figure 5: Effect of A175 on PC3 and DU145 cell motility.

Article Snippet: Human androgen independent prostate cancer PC3 and DU145 cell lines and the androgendependent LNCaP cell line were originally obtained from American Type Culture Collection (ATCC, Rockville, MD, USA); CWR22Rv1 (Rv1) cells were originally obtained from LeibnitzInstitut DSMZ (Braunschweig, Germany).

Techniques:

Journal: International journal of cancer

Article Title: MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.

doi: 10.1002/ijc.28647

Figure Lengend Snippet: Figure 5. MED15 knockdown and proliferation in PCa cells. Correlation of MED15 expression with tumor proliferation markers Ki67 and PHH3 (a). Expression of MED15 in PC3 (b) and DU145 (c) nuclear extracts by Western blot. SiRNA mediated MED15 knockdown in DU145 and PC3 cell lines by Western blot (d) and IHC (e). MTT proliferation assay of PC3 cells (f). *p < 0.05, **p < 0.01, ***p < 0.001. Similarly, proliferation assay of DU145 cells (g). *p < 0.05, **p < 0.01, ***p < 0.001. SiRNA mediated MED15 knockdown in LNCaP cell line (h). Prolif- eration assay of LNCaP cells treated with DHT (i). *p < 0.05, **p < 0.01, ***p < 0.001. ***p < 0.001, Pearson. ShRNA stable knockdown of MED15 in the DU145 cell line (j). Proliferation assay of DU145 cells with stable knockdown of MED15 compared to the scrambled control (k). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar, 10 mm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: Cell lysates and nuclear extracts of PC3 (sc-2152, Santa Cruz, CA) and DU145 (sc-24960, Santa Cruz, CA) were analyzed by immunobloting using MED15 rabbit polyclonal antibody (Proteintech, Chicago, IL).

Techniques: Knockdown, Expressing, Western Blot, Proliferation Assay, shRNA, Control

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Combined Treatment with Stattic and Docetaxel Alters the Bax/Bcl-2 Gene Expression Ratio in Human Prostate Cancer Cells

doi: 10.22034/APJCP.2016.17.11.5031

Figure Lengend Snippet: IC50 Values of Docetaxel and Stattic in DU145 Cells

Article Snippet: Human prostate cancer (DU145) cell line was obtained from National Cell Bank of Iran (Pasteur Institute, Iran).

Techniques: Incubation

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Combined Treatment with Stattic and Docetaxel Alters the Bax/Bcl-2 Gene Expression Ratio in Human Prostate Cancer Cells

doi: 10.22034/APJCP.2016.17.11.5031

Figure Lengend Snippet: DU145 Cells Exposed to Docetaxel and Stattic Treatments for 24, 48 and 72 h Determined by: MTT (A–C); Trypan Blue Cell Count (B-D). Data are Expressed as Cell Viability (%) ± SD of Three Independent Experiments.

Article Snippet: Human prostate cancer (DU145) cell line was obtained from National Cell Bank of Iran (Pasteur Institute, Iran).

Techniques: Cell Counting

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Combined Treatment with Stattic and Docetaxel Alters the Bax/Bcl-2 Gene Expression Ratio in Human Prostate Cancer Cells

doi: 10.22034/APJCP.2016.17.11.5031

Figure Lengend Snippet: Effect of Stattic, Docetaxel and Their Combination on the Expression of Bcl-2, Survivin, Mcl-1 and Bax in DU145 cells. A. The levels of Bcl-2, Survivin, Mcl-1 and Bax mRNA expression, followed by treatment with docetaxel (2 nM), stattic (2 µM) and their combination (stattic 1 µM+docetaxel 1 nM) for 24h, were determined using real-time PCR. Mean ± SD was calculated from three independent experiments(**p<0.01). B. Levels of Bcl-2, Mcl-1, Survivin and Bax expression were analyzed. Bands representing Bcl-2, Mcl-1, Survivin and Bax are shown.

Article Snippet: Human prostate cancer (DU145) cell line was obtained from National Cell Bank of Iran (Pasteur Institute, Iran).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Combined Treatment with Stattic and Docetaxel Alters the Bax/Bcl-2 Gene Expression Ratio in Human Prostate Cancer Cells

doi: 10.22034/APJCP.2016.17.11.5031

Figure Lengend Snippet: Effect of Stattic and/or Docetaxel on Bax/Bcl-2 Ratio. Bax/Bcl-2 Ratio was Calculated and Show That It was Significantly Increased in Stattic and/or Docetaxel Treated DU145 Cells (*p<0.05, **p<0.01).

Article Snippet: Human prostate cancer (DU145) cell line was obtained from National Cell Bank of Iran (Pasteur Institute, Iran).

Techniques:

Journal: Cancer Medicine

Article Title: Overexpression of claspin promotes docetaxel resistance and is associated with prostate‐specific antigen recurrence in prostate cancer

doi: 10.1002/cam4.4113

Figure Lengend Snippet: Effect of claspin inhibition on cell proliferation. (A) Western blot analysis of claspin in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs. (B) qRT‐PCR analysis of CLSPN in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs. (C) Effects of claspin knockdown on DU145 and PC3 cell proliferation. An MTT assay assessed cell proliferation at 1, 2, and 4 days after seeding on 96‐well plates. Bars and error bars indicate the mean and S.D., respectively

Article Snippet: DU145, PC3, and LNCaP were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Inhibition, Western Blot, Transfection, Negative Control, Quantitative RT-PCR, Knockdown, MTT Assay

Journal: Cancer Medicine

Article Title: Overexpression of claspin promotes docetaxel resistance and is associated with prostate‐specific antigen recurrence in prostate cancer

doi: 10.1002/cam4.4113

Figure Lengend Snippet: Association of claspin inhibition with DNA damage and resistance to docetaxel (DTX). (A) Dose‐dependent effect of DTX on the viability of DU145 and PC3 cells analyzed by MTT assay. Bars and error bars indicate the mean and S.D., respectively. (B) Western blot analysis of γ‐H2AX expressions in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs after 24 h of treatment with DTX. (C) Western blot analysis of cleaved PARP expressions in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs after 24 h of treatment with DTX

Article Snippet: DU145, PC3, and LNCaP were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Inhibition, MTT Assay, Western Blot, Transfection, Negative Control

Journal: Cancer Medicine

Article Title: Overexpression of claspin promotes docetaxel resistance and is associated with prostate‐specific antigen recurrence in prostate cancer

doi: 10.1002/cam4.4113

Figure Lengend Snippet: Effect of claspin inhibition on Akt, Erk, and ATR‐CHK1 pathways. (A) Western blot analysis of claspin, Akt, p‐Akt, Erk1/2, and p‐Erk1/2 in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs. (B) Western blot analysis of claspin, CHK1, and p‐CHK1 in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs

Article Snippet: DU145, PC3, and LNCaP were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Inhibition, Western Blot, Transfection, Negative Control

Journal: Cancer Medicine

Article Title: Overexpression of claspin promotes docetaxel resistance and is associated with prostate‐specific antigen recurrence in prostate cancer

doi: 10.1002/cam4.4113

Figure Lengend Snippet: Effect of claspin inhibition on the docetaxel (DTX)‐resistant DU145 (DU145‐DR) cell line. (A) Western blot analysis of claspin, Akt, p‐Akt, Erk1/2, and p‐Erk1/2 in DU145 and DU145‐DR cells transfected with CLSPN or negative control siRNAs. (B) Western blot analysis of claspin, CHK1, and p‐CHK1 in DU145‐DR cells transfected with CLSPN or negative control siRNAs. (C) Effects of claspin knockdown on DU145‐DR cell proliferation that was assessed by an MTT assay. (D) Dose‐dependent effect of DTX on the viability of DU145‐DR cells analyzed by MTT assay. Bars and error bars indicate the mean and S.D., respectively. (E) Western blot analysis of claspin, Akt, p‐Akt, ERK1/2, p‐ERK1/2, CHK1, and p‐CHK1 in DU145‐DR cells transfected with CLSPN or negative control siRNAs

Article Snippet: DU145, PC3, and LNCaP were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Inhibition, Western Blot, Transfection, Negative Control, Knockdown, MTT Assay