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Image Search Results
Journal: Cellular and Molecular Life Sciences: CMLS
Article Title: Ninjurin-1 executes plasma membrane rupture and impairs anti-microbial immunity in an early jawed vertebrate
doi: 10.1007/s00018-025-06020-0
Figure Lengend Snippet: Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without crosslinker disuccinimidyl suberate (DSS). Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Article Snippet: Next,
Techniques: Transfection, Staining, Fluorescence, Microscopy, Expressing, Western Blot