dss Search Results


disuccinimidyl suberate dss crosslinker  (MedChemExpress)
94
MedChemExpress disuccinimidyl suberate dss crosslinker
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Disuccinimidyl Suberate Dss Crosslinker, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chem impex international u s a single  (Chem Impex International)
95
Chem Impex International chem impex international u s a single
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Chem Impex International U S A Single, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wt vol dss molecular weight 36 000 50 000 mp biomedicals  (Valiant Co Ltd)
96
Valiant Co Ltd wt vol dss molecular weight 36 000 50 000 mp biomedicals
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Wt Vol Dss Molecular Weight 36 000 50 000 Mp Biomedicals, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wt vol dss molecular weight 36 000 50 000 mp biomedicals - by Bioz Stars, 2026-03
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rabbit polyclonal antibody against mpz  (Proteintech)
93
Proteintech rabbit polyclonal antibody against mpz
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Rabbit Polyclonal Antibody Against Mpz, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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dss  (Valiant Co Ltd)
94
Valiant Co Ltd dss
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Dss, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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dextran sodiumsulfate  (Valiant Co Ltd)
93
Valiant Co Ltd dextran sodiumsulfate
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Dextran Sodiumsulfate, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dextran sulfate sodium  (MedChemExpress)
94
MedChemExpress dextran sulfate sodium
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Dextran Sulfate Sodium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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disuccinimidyl 210 suberate  (Selleck Chemicals)
93
Selleck Chemicals disuccinimidyl 210 suberate
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Disuccinimidyl 210 Suberate, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nr0b1  (OriGene)
92
OriGene nr0b1
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Nr0b1, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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orf  (OriGene)
94
OriGene orf
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sodium 2 2 dimethyl 2 silapentane 5 sulfonate dss  (Cambridge Isotope Laboratories)
95
Cambridge Isotope Laboratories sodium 2 2 dimethyl 2 silapentane 5 sulfonate dss
Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without <t>crosslinker</t> <t>disuccinimidyl</t> suberate <t>(DSS).</t> Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1
Sodium 2 2 Dimethyl 2 Silapentane 5 Sulfonate Dss, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without crosslinker disuccinimidyl suberate (DSS). Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Ninjurin-1 executes plasma membrane rupture and impairs anti-microbial immunity in an early jawed vertebrate

doi: 10.1007/s00018-025-06020-0

Figure Lengend Snippet: Hydrophobicity and positive electrostatic potential are critical determinants of NINJ1 functionality. ( A ) HEK293T cells were transfected with mCherry-tagged WT or mutated (T79A and V118A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( B , C ) The cells transfected as described above were measured for LDH release (B) and relative fluorescence units of Sytox Green (C). Values are the means ± SD. n = 3. ** p < 0.01, and *** p < 0.001. ( D ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( E ) HEK293T cells were transfected with mCherry-tagged WT or mutated (K62A, K100A and K111A) NINJ1 for 36 h. Cell morphology and Sytox Green staining were examined by fluorescence microscopy. Scale bar, 30 μm. ( F , G ) The cells transfected as described above were measured for LDH release (F) and relative fluorescence units of Sytox Green (G). Values are the means ± SD. n = 3. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( H ) Sytox Green fluorescence of cells expressing mCherry-tagged WT or mutated NINJ1 were determined at 12, 24, 36, 48 and 60 h. ( I ) HEK293T cells expressing NINJ1 were lysed, and treated with or without crosslinker disuccinimidyl suberate (DSS). Western blot analysis was performed to determine NINJ1 oligomerization. ( J ) WT or mutated NINJ1 were expressed in HEK293T cells, and crosslinked by DSS. Western blot analysis was performed to determine oligomerization of wild type and mutated NINJ1

Article Snippet: Next, Disuccinimidyl suberate (DSS) crosslinker (MedChem Express, NJ, USA) was added to the cytoplasmic and membrane samples to a final concentration of 300 μM and incubated for 30 min at room temperature.

Techniques: Transfection, Staining, Fluorescence, Microscopy, Expressing, Western Blot