Journal: bioRxiv

Article Title: Biofilm-Derived Curli and Z-DNA Shape Anti-DNA Antibody Responses During Salmonella Infections

doi: 10.64898/2026.03.02.708989

Figure Lengend Snippet: (A-B) 129X1/SvJ mice were intragastrically infected with STm IR715 or an isogenic non-invasive mutant STm ( invAspiB ). The serum was collected 13 weeks post-infection, diluted 1:600, and assayed by ELISA for (A) anti-Z-DNA and (B) anti-B-DNA antibodies. (C-E) 129X1/SvJ mice were fed a lithogenic (high-cholesterol) diet for 8 weeks, then switched to a normal diet for 1 week before i.p. infection with STm. (C) Serum from 3 weeks post-infection was diluted 1:250 and analyzed by ELISA for anti-dsDNA autoantibodies. (D-E) Cecal tissue was stained with antibodies against Salmonella (green) and Z-DNA (blue), EBBA Biolight 680 bacterial amyloid dye for curli (red), and DAPI for host nuclei (cyan). Representative images were taken at 63X magnification on the confocal microscope and processed via ImageJ.

Article Snippet: The following commercial antibodies were used: 5μg/mL rabbit IgG isotype control (AC042), 5μg/mL mouse IgG2b isotype control (02-6300), 5μg/mL mouse anti-Z-DNA/anti-Z-RNA antibody [Z22] (Absolute Antibodies Ab00783-3.0) (Z-DNA), 5μg/mL rabbit anti-dsDNA antibody [DSD/4054R] (Novus Biologicals NBP3-07302-100ug) (B-DNA).

Techniques: Infection, Mutagenesis, Enzyme-linked Immunosorbent Assay, Staining, Microscopy