Journal: Cell reports

Article Title: AAV5-mediated manipulation of insulin expression in choroid plexus has long-term metabolic and behavioral consequences

doi: 10.1016/j.celrep.2023.112903

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal Rabbbit anti-TTR (prealbumin) AbCam Cat # ab75815; RRID:AB_1310604 Monoclonal Rabbit anti-Insulin Cell Signaling Cat # 3014; RRID:AB_2126503 Polyclonal Rabbit anti-mCherry Rockland Cat # 600-401-P16; RRID:AB_2614470 Monoclonal Rabbit anti- p -Akt (Ser473) Cell Signaling Cat # 4060; RRID:AB_2315049 Monoclonal Mouse anti-Alpha1 Na+/K+ ATPase AbCam Cat # ab7671; RRID:AB_306023 Bacterial and virus strains AAV5-CMV-CRE Vector Biolabs Cat # 7012 AAV5-CMV-GFP Vector Biolabs Cat # 7006 AAV5-CMV-Ins2 Vector Biolabs Cat # AAV-262269 AAV5-CMV-GCaMP6f Vector Biolabs Built to order AAV5-TTR-hM3Dq-mCherry Vector Biolabs Built to order AAV5-CMV-Psck1 Vector Biolabs Cat # AAV-268239 AAV5-CMV-Pcsk2 Vector Biolabs Cat # AAV-268242 Chemicals, peptides, and recombinant proteins Compound 21 TOCRIS Cat # 5548 Critical commercial assays PicoPure RNA Isolation Kit ThermoFisher Cat # KIT0204 Ultra Sensitive Insulin ELISA Kit CrystalChem Cat # 90080 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE218188 Experimental models: Cell lines iPS(IMR90)-4 WiCell WISCi004-B Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Strain # 000664 Ai14 The Jackson Laboratory Strain #007914 Ins1 −/− Ins2 fl/fl This paper N/A Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad https://graphpad.com R R Foundation for Statistical Computing https://www.r-project.org Other Normal chow diet Dyets Inc Cat# 101845 High fat/high sugar diet Dyets Inc Cat# 103806 Open in a separate window KEY RESOURCES TABLE.

Techniques: Virus, Plasmid Preparation, Recombinant, Isolation, Enzyme-linked Immunosorbent Assay, Software

Journal: bioRxiv

Article Title: Selective reduction of KCNA4 in vulnerable glutamatergic-serotonin neurons of the dorsal raphe nucleus in Alzheimer’s Disease

doi: 10.1101/2025.10.17.683113

Figure Lengend Snippet: A: Graphical overview of the htau DREADDs experiment. Htau mice were intracranially administered hM3Dq (n = 14), hM4Di (n=14), or control virus (n = 9) at 2 months’ age. DREADDs agonist (CNO DHC) was administered via water bottle for 6 weeks. B , C : RNAscope assessment of Fos expression in virally transduced Tph2 + cells before CNO washout (acute) and after 7-day washout. Two data points were collected per mouse in ( C ). No statistical test was performed in ( C ), data were qualitatively compared. D,E : Novel objection recognition (NOR). D : Percent preference for novel object and E , discrimination index. 1w ANOVA with Tukey’s post hoc . F,G : Novelty-induced suppression of feeding (NSF). F : latency to feed and G , time spent in the corners of the arena during 10-minute assessment period. H–K : Elevated plus maze (EPM) task. H : Percent of total time spent in open arm, I , entries to open arm, J , total distance traveled, and K , average velocity during the task. L–R : Three-chamber social interaction test (SIT). L : Total social interaction time, M , number of social interactions, N , social interaction time as a percentage of time spent in the social chamber, and O , time spent in the center chamber. P–R Representative heat maps, normalized within trial. D–F,H–K : 1w ANOVA w/ Dunnett’s post hoc . G , 1w Welch’s ANOVA w/Dunnett’s post hoc . L-O : 1w ANOVA w/ Tukey’s post hoc .* p < 0.05, ** p < 0.01

Article Snippet: After a 7-day recovery period, mice were continuously administered the DREADD agonist CNO DHC (clozapine-n-oxide dihydrochloride, Hello Bio #HB6149, Princeton, NJ, USA) at a concentration of 6.06 mg/ml (equivalent to 5 mg/ml CNO) via water bottle in a home-cage environment.

Techniques: Control, Virus, RNAscope, Expressing