Journal: Cell reports

Article Title: AAV5-mediated manipulation of insulin expression in choroid plexus has long-term metabolic and behavioral consequences

doi: 10.1016/j.celrep.2023.112903

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal Rabbbit anti-TTR (prealbumin) AbCam Cat # ab75815; RRID:AB_1310604 Monoclonal Rabbit anti-Insulin Cell Signaling Cat # 3014; RRID:AB_2126503 Polyclonal Rabbit anti-mCherry Rockland Cat # 600-401-P16; RRID:AB_2614470 Monoclonal Rabbit anti- p -Akt (Ser473) Cell Signaling Cat # 4060; RRID:AB_2315049 Monoclonal Mouse anti-Alpha1 Na+/K+ ATPase AbCam Cat # ab7671; RRID:AB_306023 Bacterial and virus strains AAV5-CMV-CRE Vector Biolabs Cat # 7012 AAV5-CMV-GFP Vector Biolabs Cat # 7006 AAV5-CMV-Ins2 Vector Biolabs Cat # AAV-262269 AAV5-CMV-GCaMP6f Vector Biolabs Built to order AAV5-TTR-hM3Dq-mCherry Vector Biolabs Built to order AAV5-CMV-Psck1 Vector Biolabs Cat # AAV-268239 AAV5-CMV-Pcsk2 Vector Biolabs Cat # AAV-268242 Chemicals, peptides, and recombinant proteins Compound 21 TOCRIS Cat # 5548 Critical commercial assays PicoPure RNA Isolation Kit ThermoFisher Cat # KIT0204 Ultra Sensitive Insulin ELISA Kit CrystalChem Cat # 90080 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE218188 Experimental models: Cell lines iPS(IMR90)-4 WiCell WISCi004-B Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Strain # 000664 Ai14 The Jackson Laboratory Strain #007914 Ins1 −/− Ins2 fl/fl This paper N/A Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad https://graphpad.com R R Foundation for Statistical Computing https://www.r-project.org Other Normal chow diet Dyets Inc Cat# 101845 High fat/high sugar diet Dyets Inc Cat# 103806 Open in a separate window KEY RESOURCES TABLE.

Techniques: Virus, Plasmid Preparation, Recombinant, Isolation, Enzyme-linked Immunosorbent Assay, Software

Journal: Cell Reports

Article Title: Hepatitis C virus drugs that inhibit SARS-CoV-2 papain-like protease synergize with remdesivir to suppress viral replication in cell culture

doi: 10.1016/j.celrep.2021.109133

Figure Lengend Snippet:

Article Snippet: GC-376 , Selleckchem Inc. , Cat# S0457.

Techniques: Virus, Recombinant, Purification, Fluorescence, Protease Inhibitor, Transduction, Plasmid Preparation, Expressing, Software

Journal: The Journal of Neuroscience

Article Title: Gq-Coupled Muscarinic Receptor Enhancement of KCNQ2/3 Channels and Activation of TRPC Channels in Multimodal Control of Excitability in Dentate Gyrus Granule Cells

doi: 10.1523/JNEUROSCI.1781-18.2018

Figure Lengend Snippet: Stimulation of exogenous Gq-coupled muscarinic DREADD receptors enhances IM in DGGCs. A, Confocal images of hippocampal slices immunostained for HA-tagged hM3Dq receptors (green) and counterstained with PROX1 (red), a cellular marker of DGGCs, from a Cre POMC+ mouse. The hM3Dq-HA tagged fluorescence was present in the molecular (dendrites) and granule (soma) layers of the DG, but not the hilus (mossy fiber axons). CrePOMC+ hM3Dq mice displayed exclusive expression of DREADD receptors to the somatodendritic compartment of DGGCs. The CA1 and CA3 regions did not demonstrate hM3Dq expression. Inset, Confocal micrograph from a CrePOMC− hippocampus negative control demonstrating no hM3Dq receptor expression. B, Fluorescent images show either CrePOMC+hM3Dq-mCitrine fluorescence (top) or CrePOMC− (bottom) granule layer live slices used for electrophysiology recordings obtained using a 488 nm emission filter. C, Summarized current-clamp action potential thresholds of hM3Dq-expressing DGGCs before (ACSF) and after bath-application of CNO (0.1 or 0.3 μm; n = 7 cells, *p < 0.05). D, Bars summarize the enhancement of IM by bath-application of CNO to DGGCs with transgenic insertion of hM3Dq DREADDs (Cre+ DREADD+/−, gray solid bars) specific to DG or in control littermate DGGCs not expressing hM3Dq-DREADDs from mice that lack the Cre gene (Cre− DREADD+/−, striped bars). The dashed line represents mean IM amplitude for ACSF (control) in Cre+ DREADD+/− mice. E, Summarized current-clamp action potential spike frequencies from hM3Dq-expressing DGGCs (n = 7 cells).

Article Snippet: Muscarinic DREADD (Designer Receptors Exclusively Activated by Designer Drug) mice were generated by crossing hMDq-mCitrine (Tg(CAG-CHRM3*,-mCitrine)1Ute/J, Alexander et al., 2009 ; The Jackson Laboratory, RRID: IMSR_JAX:026220 ) mice with Cre-POMC mice (Tg(Pomc1-cre)16Lowl/J; The Jackson Laboratory, RRID: IMSR_JAX:010714 ) for DGGC-specific expression ( McHugh et al., 2007 ).

Techniques: Marker, Fluorescence, Expressing, Negative Control, Transgenic Assay, Control