Journal: Aging Cell

Article Title: Aging‐associated decrease of PGC ‐1α promotes pain chronification

doi: 10.1111/acel.14177

Figure Lengend Snippet: Inhibition of interneuron activity in S1HL cortex results in hyperalgesia in older mice. (a) Representative immunostaining images. Brain slices throughout S1HL cortex were selected and stained with anti‐GABA receptor. Immunofluorescence imaging showed 80% dTomato+ cells were GABA+ cells in S1HL cortex. N = 4, data are represented as mean ± SD. (b) Diagram and sketch depict experiment design. AAV9‐Dlx‐Gi DREADD‐dTomato was injected into S1HL of adult mice. A representative image of brain slice with box region zoomed verified viral transfection in S1HL cortex. (c) Mechanical withdrawal threshold was significantly decreased after C21 administrated. (d) Hindpaw withdrawal latency in response to S1HL inhibition. Comparing with the mice subject to vector virus, mice injected Gi GREADDs exhibited significant decreasing in hindpaw withdrawal latency after C21 administration. N = 8, data are presented as mean ± SD, two‐tailed paired t test, ** p < 0.01, NS: not significant ( p > 0.05).

Article Snippet: C21 (Tocris, catalog 5548) was intraperitoneally administered at 1 mg/kg.

Techniques: Inhibition, Activity Assay, Immunostaining, Staining, Immunofluorescence, Imaging, Injection, Slice Preparation, Transfection, Plasmid Preparation, Virus, Two Tailed Test

Journal: Aging Cell

Article Title: Aging‐associated decrease of PGC ‐1α promotes pain chronification

doi: 10.1111/acel.14177

Figure Lengend Snippet: Activation of interneurons in S1HL alleviates aging‐associated pain chronification. (a) Diagram and sketch depict experimental design. AAV9‐Dlx‐Gq DREADD‐dTomato was injected into S1HL of adult mice. Following baseline behavioral test, mice received CCI surgery and C21 was administrated at day 14 after surgery. Pain behaviors were assessed 30 min after C21. (b) Mechanical withdrawal threshold. N = 8 mice, in mice received vector virus, before versus after surgery *** p < 0.001, before versus after C21 *** p < 0.001; in mice received Gq DREADD, before versus after surgery *** p < 0.001, before versus after C21 ** p < 0.01. (c) Hindpaw withdrawal latency. Comparing with mice subjected to vector virus, mice received Gq DREADD injection in S1HL and C21 administration exhibited significant increasing in mechanical withdrawal threshold and hindpaw withdrawal latency. N = 8, data are presented as mean ± SD in panel B. One‐way ANOVA followed by Tukey post hoc test was performed for the comparison. ** p < 0.01, *** p < 0.001. NS: not significant ( p > 0.05). (d) Representative immunofluorescent images. Brain slices throughout S1HL cortex were selected and stained with anti‐GABA. Immunofluorescence imaging showed 80% dTomato+ cells were GABA + cells in S1HL cortex. N = 4, data are represented as mean ± SD.

Article Snippet: C21 (Tocris, catalog 5548) was intraperitoneally administered at 1 mg/kg.

Techniques: Activation Assay, Injection, Plasmid Preparation, Virus, Comparison, Staining, Immunofluorescence, Imaging

Journal: Neurobiology of disease

Article Title: Activation of hypoactive parvalbumin-positive fast-spiking interneurons restores dentate inhibition to reduce electrographic seizures in the mouse intrahippocampal kainate model of temporal lobe epilepsy

doi: 10.1016/j.nbd.2024.106737

Figure Lengend Snippet: Chemogenetic activation of PV+ interneurons reduces in vivo electrographic seizures in the dentate gyrus of TLE PVCre::G q -DREADD mice. (A) A schematic drawing of the depth electrode location. (B) A representative image of a DAPI stained brain section from an electrode implanted mouse indicates an electrode placement in the dentate gyrus (arrows). (C) Red voltage records show 2 electrographic seizures (asterisks) before administration of CNO (i.p., 1 mg/kg). Expanded time scale of the electrographic seizure displays high-frequency spikes with high amplitude. The mouse showed only interictal spikes after receiving CNO (black voltage records). Expanded temporal scale of interictal spikes demonstrates low frequency interictal spikes. (D,E) Graphs showing significant decreases in the number (D) and cumulative duration (E) of electrographic seizures by CNO treatment (i.p., 1 mg/kg) in TLE PVCre::G q -DREADD mice ( n = 6). Electrographic seizures were measured for the 3 h before CNO administration (control) and the 3 h (left) or 23 h (right) after CNO administration. (F) A representative example of a generalized electrographic seizure observed in a kainate treated mouse, accompanied by severe convulsive (Racine Stage-5) seizures. (G) EEG record from a saline treated mouse shows no electrographic seizures. Mean ± SEM; * p < 0.05.

Article Snippet: We crossed a homozygous PVCre strain (stock #017320, The Jackson Laboratory) to a homozygous Ai32 mouse strain (expressing a ChR2/EYFP fusion protein in a Cre recombinase-dependent manner; stock #024109, The Jackson Laboratory), a homozygous G q -DREADD strain (expressing a G q -DREADD/mCitrine in a Cre recombinase-dependent manner; stock #024109, The Jackson Laboratory), or a homozygous red reporter strain (expressing a tdTomato in a Cre recombinase-dependent manner; stock #007909, The Jackson Laboratory).

Techniques: Activation Assay, In Vivo, Staining, Control, Saline