Journal: International journal of oncology

Article Title: A formulated red ginseng extract upregulates CHOP and increases TRAIL-mediated cytotoxicity in human hepatocellular carcinoma cells.

doi: 10.3892/ijo.2013.1964

Figure Lengend Snippet: Figure 4. The RGE sensitization to TRAIL-derived cell death occurs via death receptor 5 (DR5) upregulation in HCC cell lines. (A) DR5 protein levels were strongly dependent upon RGE concentration. (B) RGE‑induced upregulation of DR5, but not DR4, in HepG2 cells. (C) Upregulation of DR5 protein levels by RGE treatment in the different HCC cell lines.

Article Snippet: The following reagents were purchased and used according to the manufacturer's instructions: glutathione S-transferase (GST)-TRAIL and anti-DR5 antibodies were from Koma Biotechnologies (Seoul, Korea); anti-caspase 3, anti-PARP and anti-CHOP antibodies were from Cell Signaling Technology; anti-DR4 antibody was from Rockland; anti-tubulin antibody was from Abcam; anti-actin antibody, thapsigargin (Tg), necrostatin-1, NAC and BHA were from Sigma; and zVAD was from R&D Systems.

Techniques: Derivative Assay, Concentration Assay

Journal: Nature cell biology

Article Title: Loss of the fragile X syndrome protein FMRP results in misregulation of nonsense-mediated mRNA decay

doi: 10.1038/s41556-020-00618-1

Figure Lengend Snippet: a, Quantitations demonstrating that NMD was inhibited by each of the 3 NMD inhibitors. Samples derived from day 0, that is 24-hr after culturing iPSCs in the presence of each inhibitor as shown in Fig. 4e, b, Results are means with S.D., where n = 3 independent biological replicates. (*) P < 0.05 or (**) P < 0.01 is relative to RNA samples without NMD inhibitor (two-sided t-test). b, As in Extended Data Fig. 7j, but with or without an NMD inhibitor. Means with S.D., where n = 5 independent biological replicates. (*) P < 0.05, (**) P < 0.01 or (***) P < 0.01 is relative to RNA samples without an NMD inhibitor (two-sided t-test). c, As in Fig. 4e, but staining for MAP2 (red). d, As in Fig. 4e, but staining for BRN2/POU3F2 (green). Results are representative of 3 independent biological replicates in (c) and (d). e, Histogram representations of quantitations of western blots shown in Fig. 4f. Results represent n = 3 independent biological replicates, except for SYN1 analyses in FXS neurons, BRN2 analysis in FXS neurons treated with NMDI-1, and DCX analysis in FXS neurons treated with curcumin, where n = 2 independent biological replicates. f, Histogram representation of neurite outgrowth manifested by representative normal or FXS neurons on day 15 after differentiation. Results represent 2 independent biological replicates for FXS neurons and 3 independent biological replicates for normal neurons. All cells were stained for viability, and fluorescent signals derive from minimally 4 fields per well, where the extent of fluorescence for FXS neurons in the absence (−) of inhibitor is defined as 1. Statistical source data are provided in Source Data Extended Data Fig. 8.

Article Snippet: Coverslips were blocked with 3% bovine serum albumin (Rockland Immunochemicals) in Tris-buffered saline containing 0.1% Tween 20 (TBS-T) for 30 min at room temperature, washed once with TBS-T and incubated overnight at 4 °C in primary antibody that had been diluted in TBS-T using the following antibodies (see Supplementary Table 6 ): anti-p-UPF1 S1116 (1:250), anti-p-UPF1 S1089 (1:250), anti-UPF1 (1:500), anti-FMRP (1:250), anti-TRA-1–60 (1:500), anti-OCT4 (1:500), anti-MAP2 (1/250), anti-β3-Tubulin/TUJ1 (1:500) and anti-BRN2/POU3F2 (1:250).

Techniques: Derivative Assay, Staining, Western Blot, Fluorescence