Journal: Endocrine Reviews

Article Title: The β Cell in Diabetes: Integrating Biomarkers With Functional Measures

doi: 10.1210/endrev/bnab021

Figure Lengend Snippet: Characteristics of current human proinsulin and C-peptide assays

Article Snippet: Further advancements toward understanding β-cell prohormone processing in diabetes will require development of approaches, such as mass spectrometry ( 80 ), that are able to better discriminate the different forms in human plasma while at the same time being sensitive and specific. table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Propeptide cross-reactivity Mature peptide cross-reactivity Manufacturer Catalog number Sensitivity Intact proinsulin a split-32,33 des-31,32 split-65,66 des-64,65 Mature insulin C-peptide a Proinsulin Abcam ab242235 0.75 pmol/L - NS NS NS NS >50 ng/mL >50 ng/mL Alpco 80-PINHUT-CH01 0.455 pg/mL - NS 100% NS 100% <0.6% <0.1% EMD Millipore EZHPI-15K 0.5 pmol/L - NS 100% NS 81% >200 µU/mL >10 ng/mL Invitron IV2-002 0.02 pmol/L - 5.6% 1.4% 37% 63% 0% 0% Mercodia 10-1118-01 1.7 pmol/L - 95% 95% 90% 84% <0.03% <0.006% Meso Scale Diagnostics K1516MK 0.05 pmol/L - NS NS NS NS <0.5% 0.7% R&D Systems DPINS0 1.43 pmol/L - NS NS NS NS >3450 pmol/L NS TECOmedical Group TE1012 0.3 pmol/L - 5000 pmol/L <200 pmol/L 1000 pmol/L 200 pmol/L <10 000 pmol/L 50 000 pmol/L C-peptide Alpco 80-CPTHU-CH01 <4.32 pg/mL <0.01% NS 0.3% NS 33.2% ND - Alpco 80-CPTHU-E01.1 2.95 pmol/L 3% NS NS NS NS <0.01% - Beckman Coulter b C33451 0.01 ng/mL 3% NS NS NS NS 0% - Invitron IV2-004 5 pmol/L 2% NS NS NS NS 0% - Mercodia 10-1136-01 ≤25 pmol/L (0.076 μg/L) 2% 2% 3% 10% 74% <0.0006% - Mercodia 10-1141-01 2.5 pmol/L (0.0076 µg/L) 5% 2% 3% 10% 74% <0.0006% - Meso Scale Diagnostics K1516JK 14 pg/mL 27% ND ND ND ND 0.50% - Meso Scale Diagnostics K151X5D 4.72 pg/mL 32.4% NS NS NS NS 0.03% - Tosoh 25284 0.2 ng/mL 31.5% NS NS NS NS ND - Open in a separate window Information provided is as stated by the manufacturers and not independently verified.

Techniques:

Journal: Endocrine Reviews

Article Title: The β Cell in Diabetes: Integrating Biomarkers With Functional Measures

doi: 10.1210/endrev/bnab021

Figure Lengend Snippet: Characteristics of current human proinsulin and C-peptide assays

Article Snippet: Further advancements toward understanding β-cell prohormone processing in diabetes will require development of approaches, such as mass spectrometry ( 80 ), that are able to better discriminate the different forms in human plasma while at the same time being sensitive and specific. table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Propeptide cross-reactivity Mature peptide cross-reactivity Manufacturer Catalog number Sensitivity Intact proinsulin a split-32,33 des-31,32 split-65,66 des-64,65 Mature insulin C-peptide a Proinsulin Abcam ab242235 0.75 pmol/L - NS NS NS NS >50 ng/mL >50 ng/mL Alpco 80-PINHUT-CH01 0.455 pg/mL - NS 100% NS 100% <0.6% <0.1% EMD Millipore EZHPI-15K 0.5 pmol/L - NS 100% NS 81% >200 μU/mL >10 ng/mL Invitron IV2-002 0.02 pmol/L - 5.6% 1.4% 37% 63% 0% 0% Mercodia 10-1118-01 1.7 pmol/L - 95% 95% 90% 84% <0.03% <0.006% Meso Scale Diagnostics K1516MK 0.05 pmol/L - NS NS NS NS <0.5% 0.7% R&D Systems DPINS0 1.43 pmol/L - NS NS NS NS >3450 pmol/L NS TECOmedical Group TE1012 0.3 pmol/L - 5000 pmol/L <200 pmol/L 1000 pmol/L 200 pmol/L <10 000 pmol/L 50 000 pmol/L C-peptide Alpco 80-CPTHU-CH01 <4.32 pg/mL <0.01% NS 0.3% NS 33.2% ND - Alpco 80-CPTHU-E01.1 2.95 pmol/L 3% NS NS NS NS <0.01% - Beckman Coulter b C33451 0.01 ng/mL 3% NS NS NS NS 0% - Invitron IV2-004 5 pmol/L 2% NS NS NS NS 0% - Mercodia 10-1136-01 ≤25 pmol/L (0.076 μg/L) 2% 2% 3% 10% 74% <0.0006% - Mercodia 10-1141-01 2.5 pmol/L (0.0076 μg/L) 5% 2% 3% 10% 74% <0.0006% - Meso Scale Diagnostics K1516JK 14 pg/mL 27% ND ND ND ND 0.50% - Meso Scale Diagnostics K151X5D 4.72 pg/mL 32.4% NS NS NS NS 0.03% - Tosoh 25284 0.2 ng/mL 31.5% NS NS NS NS ND - Open in a separate window Information provided is as stated by the manufacturers and not independently verified.

Techniques: