Journal: Biofabrication

Article Title: Magnetic molding of tumor spheroids: emerging model for cancer screening.

doi: 10.1088/1758-5090/abc670

Figure Lengend Snippet: Figure 5. Doxorubicin penetration and toxicity in magnetically molded (MM) spheroids. (a) Fluorescence intensity distribution of doxorubicin for spheroids of 1 mm initial size and incubated with 10 µg ml−1 of liposomal doxorubicin for 2 h, 1 d and 3 d. The incubation with the drug was initiated at spheroid maturation times of 4 h, 1 d and 3 d. (b) Doxorubicin distribution in hanging drop sppheroids at day 2 of maturation and incubated for 1 d with the drug. Scale bars = 200 µm. (c) Doxorubicin response curves for cells in 2D culture, for magnetic spheroids of 0.5 mm and 1 mm of initial size and matured for 1 d, and for hanging drop spheroids matured for 2 d. For all conditions, doxorubicin incubation was of 3 d, and cell death % was determined with the alamarBlue™metabolic assay. Data represent mean ± SEM (n = 3).

Article Snippet: Liposomal-encapsulated doxorubicin (300112S-1EA, Avanti® Polar Lipids)was selected to study drug resistance inmagneticallymolded spheroids due to its clinical relevancy.

Techniques: Fluorescence, Incubation, Metabolic Assay

Journal: Journal of the American Chemical Society

Article Title: High-Throughput Aptamer Characterization via Real-Time Nuclease Digestion

doi: 10.1021/jacs.5c17561

Figure Lengend Snippet: Demonstrating the generalizability of RT-Exo to characterize aptamers with distinct structures binding various targets. (A) Structure of the adenosine aptamer conjugate A23T-R91–4bp. (B) RT-Exo assay data showing fluorescence over time at different concentrations of adenosine, and (C) specificity of A23T-R91–4bp as determined using the RT-Exo assay. (D) Structure of the THC aptamer conjugate THC1.2-R91–4bp. (E, F) RT-Exo assay data for THC1.2-R91–4bp (E) binding to THC and (F) specificity. (G) Structure of the cocaine aptamer conjugate MNS4.1-R91–4bp. (H, I) RT-Exo assay data for MNS4.1-R91–4bp (H) binding to cocaine and (I) specificity. THCC: (±)-11-nor-9-carboxy-Δ 9 -THC, CBN: cannabinol, THCV: tetrahydrocannabivarin, THCA: THC carboxylic acid A, CBD: cannabidiol, CBDA: cannabidiolic acid, CBGA: cannabigerolic acid, COC: cocaine, AMP: amphetamine, MTC: methcathinone, PENT: pentylone, CLO: clonazepam, CAF: caffeine, PRO: procaine, ACM: acetaminophen, IBU: ibuprofen, NIC: nicotine, BE: benzoylecgonine, MEPH: mephedrone, MDPV: methylenedioxypyrovalerone, FENT: fentanyl, METH: (+)-methamphetamine, MOR: morphine, MTP: methylphenidate, HER: heroin, PSE: pseudoephedrine, MTD: methadone, BZC: benzocaine, SCP: scopolamine, FLU: fluoxetine, SER: serotonin, DOPA: dopamine, LAC: lactose, MAN: mannitol, LIDO: lidocaine, DPH: diphenhydramine, LEV: levamisole, OXY: oxycodone, QUI: quinine, FUB: AB-FUBINACA, ALP: alprazolam, DIAZ: diazepam.

Article Snippet: Levamisole HCl was purchased from MP Biomedicals.

Techniques: Binding Assay, Fluorescence

Journal: bioRxiv

Article Title: The Reinstatement of a Forgotten Infantile Memory

doi: 10.1101/2025.09.27.678956

Figure Lengend Snippet: (A) Schematic of the behavioral protocol to trigger reinstatement of a forgotten infantile memory. Mice underwent contextual fear conditioning (cFC) training in infancy (P19), followed in adulthood by exposure to reminders of the CONTEXT and the AVERSIVE stimulus experienced during cFC training. The recall of the reinstated memory (REINSTATED recall) was tested in the CONTEXT one day after the final reminder. All behavioral sessions in adulthood were spaced by one day. (B) Freezing at CONTEXT reminder in adulthood was low in mice fear conditioned at P19, similar to animals that never experienced conditioning (Naϊve animals. Dunnett’s test, p = 0.634), and significantly higher in adult-conditioned animals (p < 0.001), indicating infantile amnesia. One-way ANOVA, F(2, 38) = 51.93, p < 0.001, r2 = 0.7321. n 2 11 mice per group. (C) At REINSTATED recall, mice conditioned at P19 expressed robust freezing (Dunnett’s test, p = 0.007), significantly higher than naϊve animals and comparable to animals conditioned during adulthood (> P60) only when both CONTEXT and AVERSIVE reminders were given. No reinstatement was observed in animals lacking either the CONTEXT (Dunnett’s test, p = 0.970) or the AVERSIVE (p > 0.999) reminder. One-way ANOVA, F(25, 226) = 11.18, p < 0.001, r2 = 0.5529; Brown-Forsythe test, F(25, 226) = 2.622, p < 0.001; Bartlett’s test, x2 = 81.17, p < 0.001. n 2 9 mice per group. (D) AAV-mediated expression of excitatory DREADD (hM3Dq-mCherry, yellow) in PV interneurons of dorsal hippocampus. Representative histological image showing mCherry expression in PV+ neurons in dorsal CA3 and CA1 (single-plane image, 10x objective; scale bar = 300 µm). (E, F) Activation of hippocampal PV interneurons via CNO injection during either the CONTEXT (E) or AVERSIVE (F) reminder prevented memory reinstatement, resulting in low freezing at REINSTATED recall. Student’s t -test against saline injected controls: CONTEXT, t = 3.515, p = 0.004, Cohen’s d = 0.507; AVERSIVE, t = 4.04, p = 0.003, Cohen’s d = 0.645. n = 6 for CONTEXT, n 2 4 for AVERSIVE.

Article Snippet: For bilateral delivery of DREADDs, PV-Cre mice received injections of pAAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene #44361-AAV1; titer: 2.2 x 1013 vg/mL) into the dorsal hippocampus (AP: −1.75 mm; ML: ±1.95 mm; DV: −1.85 mm; 350 nL per hemisphere) using a Nanoject III (Drummond).

Techniques: Expressing, Activation Assay, Injection, Saline

Journal: bioRxiv

Article Title: The Reinstatement of a Forgotten Infantile Memory

doi: 10.1101/2025.09.27.678956

Figure Lengend Snippet: We propose that reinstating forgotten infantile memories requires a carefully orchestrated hippocampus-centered network process that unfolds in three stages. First, exposure to a contextual reminder of the original experience primes the hippocampal network, increasing the recruitment of neurons associated with the forgotten infantile memory (iEngram) during subsequent experiences associated with the forgotten infantile memory (“ Priming ”, A ). Then, a reminder of the aversive stimulus experienced during infancy selectively tags iEngram neurons for offline reactivation in the following hours (“ Tagging ”, B ). Increased iEngram activity during offline network reactivation events binds previously latent infantile memory with novel neuronal ensembles, thereby reinstating behavioral responses consistent with the original experience (“ Binding ”, C ).

Article Snippet: For bilateral delivery of DREADDs, PV-Cre mice received injections of pAAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene #44361-AAV1; titer: 2.2 x 1013 vg/mL) into the dorsal hippocampus (AP: −1.75 mm; ML: ±1.95 mm; DV: −1.85 mm; 350 nL per hemisphere) using a Nanoject III (Drummond).

Techniques: Activity Assay, Binding Assay

Journal: Oncotarget

Article Title: Tumor targeting with pH-responsive poly(2-oxazoline)-based nanogels for metronomic doxorubicin treatment

doi: 10.18632/oncotarget.24806

Figure Lengend Snippet: Lysosomal cellular compartments were stained green using LysoTracker Green DND-26 and the nucleus was labeled with Hoechst 33342 (blue). The fluorescence of DOX is depicted in red and the Alexafluor label of the polymer is shown in white.

Article Snippet: Live cell CLSM images were acquired using a Zeiss LSM 880, Elyra PS.1 system (Carl Zeiss, Germany) with excitation wavelengths/emission filters of 405nm/BP 405–480 nm for Hoechst 33342, 488 nm/BP 505 to 530 nm for LysoTracker ® Green DND-26 and 488 nm/BP 585 to 615 nm for DOX and 633 nm/BP 724 to 777 nm for Alexafluor 660 ® .

Techniques: Staining, Labeling, Fluorescence