Journal: Molecules (Basel, Switzerland)

Article Title: Cytostatic Bacterial Metabolites Interfere with 5-Fluorouracil, Doxorubicin and Paclitaxel Efficiency in 4T1 Breast Cancer Cells.

doi: 10.3390/molecules29133073

Figure Lengend Snippet: Figure 9. Cadaverine and indolepropionic acid interfere with the cytostatic effect of doxorubicin. 4T1 cells were plated in 96-well plates (1500 cells/well). Cells were treated with doxorubicin alone or in combination with CAD (0.8 µM), IS (4 µM) or IPA (1 µM) for 48 h, and then cell numbers were determined by MTT assay. Data are presented as means ± SEM, from at least three biological replicates. Individual assays were measured in quadruplicate or in triplicate. Values were normalized to vehicle-treated cells (absorbance is equal to 1). Nonlinear regression (Graphpad “[Inhibitor] vs. response (four parameters)” utility) was performed on datasets to obtain IC50 and Hill slope values. Normality was determined for the inhibitory curves using the D’Agostino and Pearson normality test, while for the IC50 values and the Hill slope values the Shapiro–Wilk test was used. Statistical difference between the inhibitory curves was determined using a two-way ANOVA test, and all data points were compared with each other (in Tukey post hoc tests). For the comparison of the IC50 and Hill slope values, a non-paired, two-sided t-test was applied. ### indicates p < 0.001 for DOX-treated vs. vehicle-treated cells. * represents significance at p < 0.05 between the indicated groups. Abbreviations: CAD—cadaverine; DOX—doxorubicin; IPA—indolepropionic acid; and IS—indoxylsulfate.

Article Snippet: Liposomal Encapsuled Doxorubicin (DOX-NP, cat # 300112) was purchased from Avanti Polar Lipids (Alabaster, AL, USA) and a stock solution of 50 mM was prepared.

Techniques: MTT Assay, Comparison

Journal: Molecules

Article Title: Exploring the Interplay between Drug Release and Targeting of Lipid-Like Polymer Nanoparticles Loaded with Doxorubicin

doi: 10.3390/molecules26040831

Figure Lengend Snippet: Figure 1. Illustration of the synthesis and adsorption process involved in drug delivery using lipid-like polylactide-co- glycolide (PLGA) nanoparticles loaded with doxorubicin.

Article Snippet: Plasma doxorubicin concentrations were calculated from two calibration curves using the chemical reference standard (CRS) of doxorubicin (EP CRS, 99.0%, EDQM).

Techniques: Adsorption