Journal: Scientific Reports

Article Title: Neuroprotection by Polynitrogen Manganese Complexes: Regulation of Reactive Oxygen Species-Related Pathways

doi: 10.1038/srep20853

Figure Lengend Snippet: Cell apoptosis was detected by a flow cytometer. The neurons were pre-incubated with ( a ) neuronal medium (control), ( b ) neuronal medium, ( c ) 1 (1 μM) for 12 h ( d ) 1 (10 μM) for 12 h, ( e ) 1 (1 μM) for 24 h, ( f ) 1 (10 μM) for 24 h, ( g ) 2 (0.1 μM) for 12 h, ( h ) 2 (1 μM) for 12 h, ( i ) 2 (0.1 μM) for 24 h and ( j ) 2 (1 μM) for 24 h, and then treated with H 2 O 2 (200 μM) for 12 h ( b–j ) Statistical analysis of the apoptotic cell percentage. The data are presented as mean ± SD of three independent experiments. (Student’s T. Test, *P < 0.05, **P < 0.01 vs. group ( b )).

Article Snippet: The double staining apoptosis Kit was from KeyGEN (Nanjing, China).

Techniques: Flow Cytometry, Incubation, Control

Journal: Scientific Reports

Article Title: Neuroprotection by Polynitrogen Manganese Complexes: Regulation of Reactive Oxygen Species-Related Pathways

doi: 10.1038/srep20853

Figure Lengend Snippet: (a) PC12 cells were transfected with plasmid DNA which knocked down HIF-1α protein expression and the results was tested by western blot. (b) Statistical analysis of the percentage of HIF-1α protein expression. (c) Cell apoptosis detected by a flow cytometer. After 6 h transfection, the cells were pre-incubated with ( a ) neuronal medium (control), ( b ) neuronal medium, ( c ) 1 (10 μM) for 12 h ( d ) 1 (10 μM) for 24 h, ( e ) 2 (1 μM) for 12 h and (f) 2 (1 μM) for 24 h and then treated with H 2 O 2 (200 μM) for 12 h ( c–f ) Statistical analysis of the apoptotic cell percentage. The data are presented as mean ± SD of three independent experiments (Student’s T. Test, *P < 0.05, **P < 0.01 vs. only H 2 O 2 stimulation but without any pretreatment).

Article Snippet: The double staining apoptosis Kit was from KeyGEN (Nanjing, China).

Techniques: Transfection, Plasmid Preparation, Expressing, Western Blot, Flow Cytometry, Incubation, Control

Journal: Bioengineered

Article Title: Male-specific long non-coding RNA testis-specific transcript, Y-linked 15 promotes gastric cancer cell growth by regulating Wnt family member 1/β-catenin signaling by sponging microRNA let-7a-5p

doi: 10.1080/21655979.2022.2053814

Figure Lengend Snippet: Effect of TTTY15 on GC cell proliferation, apoptosis, migration, invasion and EMT process. (a) The transfection efficiency of TTTY15 overexpression plasmid, si-TTTY15-1 and si-TTTY15-2 was detected by qRT-PCR. (b and c) CCK-8 method and BrdU assay were conducted for detecting the effects of TTTY15 overexpression and knockdown on the viability and proliferation of NCI-N87 and SUN-1 cells. (d) Flow cytometry assay was utilized for detecting the effects of TTTY15 overexpression and knockdown on the apoptosis of NCI-N87 and SUN-1 cells. (e) Transwell assays were performed to detect the effects of TTTY15 overexpression and knockdown on the migration and invasion of NCI-N87 and SUN-1 cells. (f) Western blot was carried out to detect the effects of TTTY15 overexpression and knockdown on the expression of EMT-related proteins in NCI-N87 and SUN-1 cells. All of the experiments were performed in triplicate. * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: The AnnexinV-FITC/PI cell apoptosis double staining kit (BD Biosciences, New Jersey, USA) was employed for detecting cell apoptosis.

Techniques: Migration, Transfection, Over Expression, Plasmid Preparation, Quantitative RT-PCR, CCK-8 Assay, BrdU Staining, Flow Cytometry, Western Blot, Expressing

Journal: Bioengineered

Article Title: Male-specific long non-coding RNA testis-specific transcript, Y-linked 15 promotes gastric cancer cell growth by regulating Wnt family member 1/β-catenin signaling by sponging microRNA let-7a-5p

doi: 10.1080/21655979.2022.2053814

Figure Lengend Snippet: Effects of TTTY15/let-7a-5p axis on GC cell proliferation, apoptosis, migration and invasion. (a) qRT-PCR was used to detect the transfection efficiency of TTTY15 overexpression plasmids + miR mimics and si-TTTY15-1 + miR inhibitors. (b and c) CCK-8 method and BrdU assay were used to detect the effects of TTTY15 and let-7a-5p on the viability and proliferation of NCI-N87 and SUN-1cells. (d) Flow cytometry assay was used to detect the effects of TTTY15 and let-7a-5p on NCI-N87 and SUN-1 cell apoptosis. (e and f) Transwell assays were used to detect the effects of TTTY15 and let-7a-5p on the migration and invasion of NCI-N87 and SUN-1 cells. (g) Western blot was used to detect the effects of TTTY15 and let-7a-5p on the EMT process of NCI-N87 and SUN-1 cells. All of the experiments were performed in triplicate. ** P < 0.01 and *** P < 0.001.

Article Snippet: The AnnexinV-FITC/PI cell apoptosis double staining kit (BD Biosciences, New Jersey, USA) was employed for detecting cell apoptosis.

Techniques: Migration, Quantitative RT-PCR, Transfection, Over Expression, CCK-8 Assay, BrdU Staining, Flow Cytometry, Western Blot