dose response function fitting in originpro 9.7 Search Results


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Santa Cruz Biotechnology anti mif polyclonal antibody
A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or <t>anti-MIF</t> <t>polyclonal</t> antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.
Anti Mif Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or <t>anti-MIF</t> <t>polyclonal</t> antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.
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wavemetrics inc igorpro
A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or <t>anti-MIF</t> <t>polyclonal</t> antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.
Igorpro, supplied by wavemetrics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SYSTAT sigmaplot
A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or <t>anti-MIF</t> <t>polyclonal</t> antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.
Sigmaplot, supplied by SYSTAT, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RStudio drc package
A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or <t>anti-MIF</t> <t>polyclonal</t> antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.
Drc Package, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CH Instruments probit dose–response model
A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or <t>anti-MIF</t> <t>polyclonal</t> antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.
Probit Dose–Response Model, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ERITHACUS SOFTWARE LIMITED grafit
A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or <t>anti-MIF</t> <t>polyclonal</t> antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.
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OriginLab corp dose–response curve fit
A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or <t>anti-MIF</t> <t>polyclonal</t> antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.
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OriginLab corp sigmoidal dose-response curve origin 6.1
A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or <t>anti-MIF</t> <t>polyclonal</t> antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.
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Image Search Results


A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or anti-MIF polyclonal antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.

Journal: PLoS ONE

Article Title: Pro-Inflammatory Action of MIF in Acute Myocardial Infarction via Activation of Peripheral Blood Mononuclear Cells

doi: 10.1371/journal.pone.0076206

Figure Lengend Snippet: A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or anti-MIF polyclonal antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.

Article Snippet: To investigate the effect of anti-MIF intervention on inflammatory responses, animals were treated with a single dose of anti-MIF polyclonal antibody (Santa Cruz, sc-20121) or isotype control rabbit IgG (Santa Cruz, sc-2027), at 5 mg/kg i.p. immediately after CAO.

Techniques: Immunohistochemical staining, Staining, Control, Western Blot, Expressing