Journal: International Journal of Molecular Sciences

Article Title: Eckol Alleviates Intestinal Dysfunction during Suckling-to-Weaning Transition via Modulation of PDX1 and HBEGF

doi: 10.3390/ijms21134755

Figure Lengend Snippet: Eckol protects against H 2 O 2 -induced oxidative stress in IPEC-J2 cells. Relative HO-1 ( A ) and MnSOD expression levels ( C ) were evaluated through qRT-PCR (n = 3). Relative cellular ROS ratio was determined through 2’,7’-dichlorofluorescein (DCF) fluorescence analysis with or without PI3K/AKT (LY2944002), P38 (SB202190) ( B ), or 5’-AMP-activated protein kinase (AMPK) ( D ) inhibitor (n = 3) in eckol-treated cells exposed to H 2 O 2 -induced oxidative stress. Lower-case letters indicate significant differences ( p < 0.05) among treatments, based on Duncan’s multiple range test.

Article Snippet: The IPEC-J2 cells were incubated with different treatments, including recombinant HBEGF (Mybiosource, San Diego, CA, USA), LY2944002 (Cell Signaling Technology, Danvers, MA, USA), SB202190 (Cell Signaling Technology, USA), and the AMPK inhibitor (dorsomorphin dihydrochloride; Santa Cruz Biotechnology, Dallas, TX, USA) at different intervals, as indicated in the figure legends.

Techniques: Expressing, Quantitative RT-PCR, Fluorescence

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: Drp1 acetylation mediated by CDK5-AMPK-GCN5L1 axis promotes cerebral ischemic injury via facilitating mitochondrial fission.

doi: 10.1186/s10020-024-00948-y

Figure Lengend Snippet: Fig. 8 Inhibition of AMPK by Compound C protects brain tissues from ischemic damage in mouse models of dMCAO. (A) Representative TTC-stained brain sections and quantitative analysis of infarct volume (n=3). (B) Western blot analysis of p-Drp1 and p-AMPK in brain tissues of dMCAO and control mice treated with or without Compound C (n=3). (C) Immunohistochemistry staining for p-Drp1 and p-AMPK in brain tissues of dMCAO and control mice treated as in (B). Scale bars represent 100 μm (n=3). (D) Acetylated Drp1 was analyzed in brain tissues of dMCAO and control mice treated as in (B) by IP with anti-acetyl lysine antibody and western blotting with anti-Drp1 (n=3). (E) Fluorescence images of MitoSOX staining in brain sections of dMCAO and control mice treated as in (B). Scale bars represent 500 μm (n=3). (F) mtROS levels were analyzed by flow cytometry. Bar graphs show the mtROS levels measured based on fluorescence intensity (n=3). (G) TUNEL staining detected apoptosis in brain tissues of dMCAO and control mice treated as in (B). Scale bars represent 200 μm (n=3). Data are represented as mean ± SD, *P<0.05, **P<0.01, ****P<0.0001, P-value was determined by one-way ANOVA with Dunnett’s post hoc correction

Article Snippet: Compound C (inhibitor of AMPK) (5 μM, MCE, HY-13418 A) was acquired from MCE and dissolved in DMSO.

Techniques: Inhibition, Staining, Western Blot, Control, Immunohistochemistry, Fluorescence, Flow Cytometry, TUNEL Assay