donors Search Results


single donor human red blood cells  (Innovative Research Inc)
94
Innovative Research Inc single donor human red blood cells
Single Donor Human Red Blood Cells, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single donor human red blood cells/product/Innovative Research Inc
Average 94 stars, based on 1 article reviews
single donor human red blood cells - by Bioz Stars, 2026-02
94/100 stars
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chondrocytes  (StemBioSys)
91
StemBioSys chondrocytes
Chondrocytes, supplied by StemBioSys, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chondrocytes/product/StemBioSys
Average 91 stars, based on 1 article reviews
chondrocytes - by Bioz Stars, 2026-02
91/100 stars
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psc101 donor  (Addgene inc)
93
Addgene inc psc101 donor
Psc101 Donor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psc101 donor/product/Addgene inc
Average 93 stars, based on 1 article reviews
psc101 donor - by Bioz Stars, 2026-02
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pdonor egp2ap rc  (Addgene inc)
93
Addgene inc pdonor egp2ap rc
Pdonor Egp2ap Rc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pdonor egp2ap rc - by Bioz Stars, 2026-02
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glutathione donor alphascreen beads  (Revvity)
92
Revvity glutathione donor alphascreen beads
CRISPR/Cas9-induced mutagenesis generates a TRN-SR2/IN interface mutant but does not affect nuclear import of SR proteins. <t>AlphaScreen-based</t> analysis of the interaction between (a) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 (corresponding to TRN-SR2 protein expressed in clone 15.15) with GST-tagged integrase (IN). TRN-SR2 protein was titrated over a fixed concentration of IN (80 nM). (b) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 with GST-tagged ASF/SF2. TRN-SR2 protein was titrated over a fixed concentration of ASF/SF2 (30 nM). Mean and standard deviation are shown from one of two representative experiments, performed in duplicate. (c and d) Control (HeLaP4 WT), HeLaP4 TRN-SR2 KD, HeLaP4 clones, and back-complemented cells were fixed and immunostained with monoclonal antibodies against (c) ASF/SF2 (an automated linear adjustment of brightness and contrast was applied on the entire images using Fiji) and (d) CPSF6. Secondary antibody was conjugated to Alexa 488 (pseudocolored green) while the nucleus was stained with DAPI (pseudocolored blue). Scale bars represent 10 μm. One representative experiment, including the parallel analysis of all cell lines, of two experiments is shown.
Glutathione Donor Alphascreen Beads, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glutathione donor alphascreen beads/product/Revvity
Average 92 stars, based on 1 article reviews
glutathione donor alphascreen beads - by Bioz Stars, 2026-02
92/100 stars
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ptead1 donor  (Addgene inc)
93
Addgene inc ptead1 donor
CRISPR/Cas9-induced mutagenesis generates a TRN-SR2/IN interface mutant but does not affect nuclear import of SR proteins. <t>AlphaScreen-based</t> analysis of the interaction between (a) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 (corresponding to TRN-SR2 protein expressed in clone 15.15) with GST-tagged integrase (IN). TRN-SR2 protein was titrated over a fixed concentration of IN (80 nM). (b) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 with GST-tagged ASF/SF2. TRN-SR2 protein was titrated over a fixed concentration of ASF/SF2 (30 nM). Mean and standard deviation are shown from one of two representative experiments, performed in duplicate. (c and d) Control (HeLaP4 WT), HeLaP4 TRN-SR2 KD, HeLaP4 clones, and back-complemented cells were fixed and immunostained with monoclonal antibodies against (c) ASF/SF2 (an automated linear adjustment of brightness and contrast was applied on the entire images using Fiji) and (d) CPSF6. Secondary antibody was conjugated to Alexa 488 (pseudocolored green) while the nucleus was stained with DAPI (pseudocolored blue). Scale bars represent 10 μm. One representative experiment, including the parallel analysis of all cell lines, of two experiments is shown.
Ptead1 Donor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ptead1 donor/product/Addgene inc
Average 93 stars, based on 1 article reviews
ptead1 donor - by Bioz Stars, 2026-02
93/100 stars
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pmx klf4  (Addgene inc)
90
Addgene inc pmx klf4
CRISPR/Cas9-induced mutagenesis generates a TRN-SR2/IN interface mutant but does not affect nuclear import of SR proteins. <t>AlphaScreen-based</t> analysis of the interaction between (a) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 (corresponding to TRN-SR2 protein expressed in clone 15.15) with GST-tagged integrase (IN). TRN-SR2 protein was titrated over a fixed concentration of IN (80 nM). (b) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 with GST-tagged ASF/SF2. TRN-SR2 protein was titrated over a fixed concentration of ASF/SF2 (30 nM). Mean and standard deviation are shown from one of two representative experiments, performed in duplicate. (c and d) Control (HeLaP4 WT), HeLaP4 TRN-SR2 KD, HeLaP4 clones, and back-complemented cells were fixed and immunostained with monoclonal antibodies against (c) ASF/SF2 (an automated linear adjustment of brightness and contrast was applied on the entire images using Fiji) and (d) CPSF6. Secondary antibody was conjugated to Alexa 488 (pseudocolored green) while the nucleus was stained with DAPI (pseudocolored blue). Scale bars represent 10 μm. One representative experiment, including the parallel analysis of all cell lines, of two experiments is shown.
Pmx Klf4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmx klf4/product/Addgene inc
Average 90 stars, based on 1 article reviews
pmx klf4 - by Bioz Stars, 2026-02
90/100 stars
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cells hskmdc  (Cook MyoSite Inc)
94
Cook MyoSite Inc cells hskmdc
CRISPR/Cas9-induced mutagenesis generates a TRN-SR2/IN interface mutant but does not affect nuclear import of SR proteins. <t>AlphaScreen-based</t> analysis of the interaction between (a) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 (corresponding to TRN-SR2 protein expressed in clone 15.15) with GST-tagged integrase (IN). TRN-SR2 protein was titrated over a fixed concentration of IN (80 nM). (b) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 with GST-tagged ASF/SF2. TRN-SR2 protein was titrated over a fixed concentration of ASF/SF2 (30 nM). Mean and standard deviation are shown from one of two representative experiments, performed in duplicate. (c and d) Control (HeLaP4 WT), HeLaP4 TRN-SR2 KD, HeLaP4 clones, and back-complemented cells were fixed and immunostained with monoclonal antibodies against (c) ASF/SF2 (an automated linear adjustment of brightness and contrast was applied on the entire images using Fiji) and (d) CPSF6. Secondary antibody was conjugated to Alexa 488 (pseudocolored green) while the nucleus was stained with DAPI (pseudocolored blue). Scale bars represent 10 μm. One representative experiment, including the parallel analysis of all cell lines, of two experiments is shown.
Cells Hskmdc, supplied by Cook MyoSite Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cells hskmdc/product/Cook MyoSite Inc
Average 94 stars, based on 1 article reviews
cells hskmdc - by Bioz Stars, 2026-02
94/100 stars
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cdk9 halo repair template  (Addgene inc)
93
Addgene inc cdk9 halo repair template
CRISPR/Cas9-induced mutagenesis generates a TRN-SR2/IN interface mutant but does not affect nuclear import of SR proteins. <t>AlphaScreen-based</t> analysis of the interaction between (a) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 (corresponding to TRN-SR2 protein expressed in clone 15.15) with GST-tagged integrase (IN). TRN-SR2 protein was titrated over a fixed concentration of IN (80 nM). (b) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 with GST-tagged ASF/SF2. TRN-SR2 protein was titrated over a fixed concentration of ASF/SF2 (30 nM). Mean and standard deviation are shown from one of two representative experiments, performed in duplicate. (c and d) Control (HeLaP4 WT), HeLaP4 TRN-SR2 KD, HeLaP4 clones, and back-complemented cells were fixed and immunostained with monoclonal antibodies against (c) ASF/SF2 (an automated linear adjustment of brightness and contrast was applied on the entire images using Fiji) and (d) CPSF6. Secondary antibody was conjugated to Alexa 488 (pseudocolored green) while the nucleus was stained with DAPI (pseudocolored blue). Scale bars represent 10 μm. One representative experiment, including the parallel analysis of all cell lines, of two experiments is shown.
Cdk9 Halo Repair Template, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdk9 halo repair template/product/Addgene inc
Average 93 stars, based on 1 article reviews
cdk9 halo repair template - by Bioz Stars, 2026-02
93/100 stars
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plasma pooled hiv  (Lee Biosolutions)
91
Lee Biosolutions plasma pooled hiv
CRISPR/Cas9-induced mutagenesis generates a TRN-SR2/IN interface mutant but does not affect nuclear import of SR proteins. <t>AlphaScreen-based</t> analysis of the interaction between (a) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 (corresponding to TRN-SR2 protein expressed in clone 15.15) with GST-tagged integrase (IN). TRN-SR2 protein was titrated over a fixed concentration of IN (80 nM). (b) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 with GST-tagged ASF/SF2. TRN-SR2 protein was titrated over a fixed concentration of ASF/SF2 (30 nM). Mean and standard deviation are shown from one of two representative experiments, performed in duplicate. (c and d) Control (HeLaP4 WT), HeLaP4 TRN-SR2 KD, HeLaP4 clones, and back-complemented cells were fixed and immunostained with monoclonal antibodies against (c) ASF/SF2 (an automated linear adjustment of brightness and contrast was applied on the entire images using Fiji) and (d) CPSF6. Secondary antibody was conjugated to Alexa 488 (pseudocolored green) while the nucleus was stained with DAPI (pseudocolored blue). Scale bars represent 10 μm. One representative experiment, including the parallel analysis of all cell lines, of two experiments is shown.
Plasma Pooled Hiv, supplied by Lee Biosolutions, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasma pooled hiv/product/Lee Biosolutions
Average 91 stars, based on 1 article reviews
plasma pooled hiv - by Bioz Stars, 2026-02
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human nasal fluid  (Lee Biosolutions)
94
Lee Biosolutions human nasal fluid
CRISPR/Cas9-induced mutagenesis generates a TRN-SR2/IN interface mutant but does not affect nuclear import of SR proteins. <t>AlphaScreen-based</t> analysis of the interaction between (a) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 (corresponding to TRN-SR2 protein expressed in clone 15.15) with GST-tagged integrase (IN). TRN-SR2 protein was titrated over a fixed concentration of IN (80 nM). (b) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 with GST-tagged ASF/SF2. TRN-SR2 protein was titrated over a fixed concentration of ASF/SF2 (30 nM). Mean and standard deviation are shown from one of two representative experiments, performed in duplicate. (c and d) Control (HeLaP4 WT), HeLaP4 TRN-SR2 KD, HeLaP4 clones, and back-complemented cells were fixed and immunostained with monoclonal antibodies against (c) ASF/SF2 (an automated linear adjustment of brightness and contrast was applied on the entire images using Fiji) and (d) CPSF6. Secondary antibody was conjugated to Alexa 488 (pseudocolored green) while the nucleus was stained with DAPI (pseudocolored blue). Scale bars represent 10 μm. One representative experiment, including the parallel analysis of all cell lines, of two experiments is shown.
Human Nasal Fluid, supplied by Lee Biosolutions, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human nasal fluid/product/Lee Biosolutions
Average 94 stars, based on 1 article reviews
human nasal fluid - by Bioz Stars, 2026-02
94/100 stars
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human saliva  (Lee Biosolutions)
93
Lee Biosolutions human saliva
CRISPR/Cas9-induced mutagenesis generates a TRN-SR2/IN interface mutant but does not affect nuclear import of SR proteins. <t>AlphaScreen-based</t> analysis of the interaction between (a) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 (corresponding to TRN-SR2 protein expressed in clone 15.15) with GST-tagged integrase (IN). TRN-SR2 protein was titrated over a fixed concentration of IN (80 nM). (b) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 with GST-tagged ASF/SF2. TRN-SR2 protein was titrated over a fixed concentration of ASF/SF2 (30 nM). Mean and standard deviation are shown from one of two representative experiments, performed in duplicate. (c and d) Control (HeLaP4 WT), HeLaP4 TRN-SR2 KD, HeLaP4 clones, and back-complemented cells were fixed and immunostained with monoclonal antibodies against (c) ASF/SF2 (an automated linear adjustment of brightness and contrast was applied on the entire images using Fiji) and (d) CPSF6. Secondary antibody was conjugated to Alexa 488 (pseudocolored green) while the nucleus was stained with DAPI (pseudocolored blue). Scale bars represent 10 μm. One representative experiment, including the parallel analysis of all cell lines, of two experiments is shown.
Human Saliva, supplied by Lee Biosolutions, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human saliva/product/Lee Biosolutions
Average 93 stars, based on 1 article reviews
human saliva - by Bioz Stars, 2026-02
93/100 stars
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Image Search Results


CRISPR/Cas9-induced mutagenesis generates a TRN-SR2/IN interface mutant but does not affect nuclear import of SR proteins. AlphaScreen-based analysis of the interaction between (a) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 (corresponding to TRN-SR2 protein expressed in clone 15.15) with GST-tagged integrase (IN). TRN-SR2 protein was titrated over a fixed concentration of IN (80 nM). (b) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 with GST-tagged ASF/SF2. TRN-SR2 protein was titrated over a fixed concentration of ASF/SF2 (30 nM). Mean and standard deviation are shown from one of two representative experiments, performed in duplicate. (c and d) Control (HeLaP4 WT), HeLaP4 TRN-SR2 KD, HeLaP4 clones, and back-complemented cells were fixed and immunostained with monoclonal antibodies against (c) ASF/SF2 (an automated linear adjustment of brightness and contrast was applied on the entire images using Fiji) and (d) CPSF6. Secondary antibody was conjugated to Alexa 488 (pseudocolored green) while the nucleus was stained with DAPI (pseudocolored blue). Scale bars represent 10 μm. One representative experiment, including the parallel analysis of all cell lines, of two experiments is shown.

Journal: Microbiology Spectrum

Article Title: CRISPR/Cas9-Induced Mutagenesis Corroborates the Role of Transportin-SR2 in HIV-1 Nuclear Import

doi: 10.1128/Spectrum.01336-21

Figure Lengend Snippet: CRISPR/Cas9-induced mutagenesis generates a TRN-SR2/IN interface mutant but does not affect nuclear import of SR proteins. AlphaScreen-based analysis of the interaction between (a) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 (corresponding to TRN-SR2 protein expressed in clone 15.15) with GST-tagged integrase (IN). TRN-SR2 protein was titrated over a fixed concentration of IN (80 nM). (b) His 6 -tagged WT TRN-SR2 or His 6 -tagged ΔV 105 TRN-SR2 with GST-tagged ASF/SF2. TRN-SR2 protein was titrated over a fixed concentration of ASF/SF2 (30 nM). Mean and standard deviation are shown from one of two representative experiments, performed in duplicate. (c and d) Control (HeLaP4 WT), HeLaP4 TRN-SR2 KD, HeLaP4 clones, and back-complemented cells were fixed and immunostained with monoclonal antibodies against (c) ASF/SF2 (an automated linear adjustment of brightness and contrast was applied on the entire images using Fiji) and (d) CPSF6. Secondary antibody was conjugated to Alexa 488 (pseudocolored green) while the nucleus was stained with DAPI (pseudocolored blue). Scale bars represent 10 μm. One representative experiment, including the parallel analysis of all cell lines, of two experiments is shown.

Article Snippet: Next, 10 μl of a mixture of Ni2 + chelate acceptor and glutathione donor AlphaScreen beads (PerkinElmer Life Sciences) was added, at a final concentration of 0.02 mg/ml for each of the beads.

Techniques: CRISPR, Mutagenesis, Amplified Luminescent Proximity Homogenous Assay, Concentration Assay, Standard Deviation, Control, Clone Assay, Staining