Journal: SpringerPlus

Article Title: A reliable and effective method of DNA isolation from old human blood paper cards

doi: 10.1186/2193-1801-2-616

Figure Lengend Snippet: Concentrations, A260/A280 and A260/A230 ratios of DNA isolated from old human blood paper cards with the four different reagents (DN131, Tris–HCl, TE, H 2 O). A . DNA concentrations (ng/μl); B . A260/A280 ratios; C . A260/A230 ratios. *, p < 0.05, compared to DN131 group. T -test was performed for DNA concentrations and A260/A230 ratios because these data are normally distributed, while A260/A280 data are not normally distributed. Wilcoxon Rank-Sum test was used to examine the A260/A280 differences between methods including DN131.

Article Snippet: In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago.

Techniques: Isolation

Journal: SpringerPlus

Article Title: A reliable and effective method of DNA isolation from old human blood paper cards

doi: 10.1186/2193-1801-2-616

Figure Lengend Snippet: Bland-Altman Plots of DNA concentrations, A260/A280 and A260/A230 ratios between the two methods using DN131 and using our solutions (Tris–HCl, TE or water). A . Bland-Altman Plot of DNA concentrations between DN131 and Tris–HCl. Correlation R = 0.38 ( p < 0.0001), slope = − 0.76 ( p < 0.001), intercept = 193.27 ( p < 0.0001); B . Bland-Altman Plot of DNA concentrations between DN131 and TE. Correlation R = 0.28 ( p < 0.004), slope = − 0.46 ( p = 0.004), intercept = 145.59 ( p < 0.0001); C . Bland-Altman Plot of DNA concentrations between DN131 and H 2 O. Correlation R = 0.05 ( p = 0.55), slope = 0.32 ( p = 0.96), intercept = 59.65 ( p = 0.99); D . Bland-Altman Plot of A260/A280 ratios between DN131 and Tris–HCl. Correlation R = 0.99 ( p < 0.0001), slope = − 2.0 ( p < 0.0001), intercept = 2.95 ( p < 0.0001); E . Bland-Altman Plot of A260/A280 ratios between DN131 and TE. Correlation R = 0.97 ( p < 0.0001), slope = − 1.97 ( p < 0.0001), intercept = 3.51 ( p < 0.0001); F . Bland-Altman Plot of A260/A280 ratios between DN131 and H 2 O. Correlation R = 0.99 ( p < 0.0001), slope = −20 ( p < 0.0001), intercept = 2.91 ( p < 0.0001); G . Bland-Altman Plot of A260/A230 ratios between DN131 and Tris–HCl. Correlation R = 0.06 ( p = 0.57), slope = 0.64 ( p = 0.99), intercept = 0.27 ( p = 0.99); H . Bland-Altman Plot of A260/A230 ratios between DN131 and TE. Correlation R = 0.99 ( p < 0.0001), slope = 1.99 ( p < 0.0001), intercept = −0.12 ( p = 0.007); I . Bland-Altman Plot of A260/A230 ratios between DN131 and H 2 O. Correlation R = 0.47 ( p < 0.0001), slope = 0.97 ( p < 0.0001), intercept = 0.13 ( p = 0.007).

Article Snippet: In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago.

Techniques:

Journal: SpringerPlus

Article Title: A reliable and effective method of DNA isolation from old human blood paper cards

doi: 10.1186/2193-1801-2-616

Figure Lengend Snippet: PCR amplification of a DNA fragment of caspase-12 gene covering C125T SNP (rs497116) using 105 DNA samples isolated by different reagents (DN131, Tris–HCl, TE and water) with two different PCR amplification systems. A . A representative image of PCR amplified bands separated by a 2% agarose gel electrophoresis for system 1, which was detailed in Materials and methods. PCR bands were visualized under UV light after ethidium bromide staining; B . PCR band density quantification by Image J for Figure 3A. *, p < 0.05; **, p < 0.01, compared to DN131; C . A representative image of PCR amplified bands separated by a 2% agarose gel electrophoresis for system 2, which was detailed in Materials and methods; D . PCR band density quantification by Image J for Figure 3C. ***, p < 0.001, compared to DN131.

Article Snippet: In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago.

Techniques: Amplification, Isolation, Agarose Gel Electrophoresis, Staining

Journal: SpringerPlus

Article Title: A reliable and effective method of DNA isolation from old human blood paper cards

doi: 10.1186/2193-1801-2-616

Figure Lengend Snippet: Success rates of amplifying caspase-12 C125T SNP (rs497116) by PCR and a Taqman genotyping assay using four different reagents

Article Snippet: In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago.

Techniques: Genotyping Assay