dnastar lasergene software package Search Results


seqman ngen software v14  (DNASTAR)
97
DNASTAR seqman ngen software v14
Summary of the pipeline methodologies (tx: Treatment).
Seqman Ngen Software V14, supplied by DNASTAR, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/SeqMan+NGen/pmc06770386-31-10-14
Average 97 stars, based on 1 article reviews
seqman ngen software v14 - by Bioz Stars, 2026-09
97/100 stars
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megalign 7 2 1  (DNASTAR)
99
DNASTAR megalign 7 2 1
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Megalign 7 2 1, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/MegAlign/pmc03973953-121-21-23
Average 99 stars, based on 1 article reviews
megalign 7 2 1 - by Bioz Stars, 2026-09
99/100 stars
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lasergene genomic suite v 5 software package  (DNASTAR)
94
DNASTAR lasergene genomic suite v 5 software package
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Lasergene Genomic Suite V 5 Software Package, supplied by DNASTAR, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/Lasergene+Genomics+Suite/pmc04959555-166-7-14
Average 94 stars, based on 1 article reviews
lasergene genomic suite v 5 software package - by Bioz Stars, 2026-09
94/100 stars
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lasergene molecular biology suite software  (DNASTAR)
93
DNASTAR lasergene molecular biology suite software
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Lasergene Molecular Biology Suite Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/Lasergene+Molecular+Biology+Suite/pmc06292373-107-12-17
Average 93 stars, based on 1 article reviews
lasergene molecular biology suite software - by Bioz Stars, 2026-09
93/100 stars
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lasergene software package v 7 0 0  (DNASTAR)
99
DNASTAR lasergene software package v 7 0 0
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Lasergene Software Package V 7 0 0, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/Lasergene/pm20203289-57-3-7
Average 99 stars, based on 1 article reviews
lasergene software package v 7 0 0 - by Bioz Stars, 2026-09
99/100 stars
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seqman pro software version 7 2 2  (DNASTAR)
97
DNASTAR seqman pro software version 7 2 2
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Seqman Pro Software Version 7 2 2, supplied by DNASTAR, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/SeqMan+Pro/pm22189211-166-34-39
Average 97 stars, based on 1 article reviews
seqman pro software version 7 2 2 - by Bioz Stars, 2026-09
97/100 stars
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lasergene primerselect software  (DNASTAR)
96
DNASTAR lasergene primerselect software
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Lasergene Primerselect Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/PrimerSelect/10__1074_slash_jbc__m110__100636-82-5-8
Average 96 stars, based on 1 article reviews
lasergene primerselect software - by Bioz Stars, 2026-09
96/100 stars
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seqman software  (Lasergen Inc)
90
Lasergen Inc seqman software
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Seqman Software, supplied by Lasergen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/seqman+software/pmc07570136-371-15-18
Average 90 stars, based on 1 article reviews
seqman software - by Bioz Stars, 2026-09
90/100 stars
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lasergene megalign pro software  (DNASTAR)
96
DNASTAR lasergene megalign pro software
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Lasergene Megalign Pro Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/MegAlign+Pro/pm37012225-129-19-23
Average 96 stars, based on 1 article reviews
lasergene megalign pro software - by Bioz Stars, 2026-09
96/100 stars
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rna folding predictive algorithm  (DNASTAR)
94
DNASTAR rna folding predictive algorithm
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Rna Folding Predictive Algorithm, supplied by DNASTAR, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/GeneQuest/pmc02190293-181-6-11
Average 94 stars, based on 1 article reviews
rna folding predictive algorithm - by Bioz Stars, 2026-09
94/100 stars
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seqbuilderpro  (DNASTAR)
95
DNASTAR seqbuilderpro
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Seqbuilderpro, supplied by DNASTAR, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/SeqBuilder+Pro/pmc12230683-24-21-23
Average 95 stars, based on 1 article reviews
seqbuilderpro - by Bioz Stars, 2026-09
95/100 stars
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megalin program  (Lasergen Inc)
90
Lasergen Inc megalin program
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Megalin Program, supplied by Lasergen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+lasergene+software+package/megalin+program/pmc04242230-97-20-23
Average 90 stars, based on 1 article reviews
megalin program - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Summary of the pipeline methodologies (tx: Treatment).

Journal: Genes

Article Title: Viral Metagenomics in the Clinical Realm: Lessons Learned from a Swiss-Wide Ring Trial

doi: 10.3390/genes10090655

Figure Lengend Snippet: Summary of the pipeline methodologies (tx: Treatment).

Article Snippet: Tool , bbmap bbsplit , , Kraken2 + Bowtie2 , SeqMan NGen software v14 (DNAStar, Lasergene) , bowtie2 , Kraken , bwa mem, blastn , SNAP.

Techniques: Extraction, Homogenization, Centrifugation, Filtration, DNA Extraction, RNA Extraction, DNA Library Preparation, Software

Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in MegAlign 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons

Journal: Applied Microbiology and Biotechnology

Article Title: Recombinant Aspergillus β-galactosidases as a robust glycomic and biotechnological tool

doi: 10.1007/s00253-013-5192-3

Figure Lengend Snippet: Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in MegAlign 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons

Article Snippet: Fig. 1 Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in MegAlign 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database.

Techniques: SDS Page, Recombinant, Software, Purification, Staining, Incubation, Molecular Weight