Journal: Frontiers in Plant Science

Article Title: StMAPKK5 responds to heat stress by regulating potato growth, photosynthesis, and antioxidant defenses

doi: 10.3389/fpls.2024.1392425

Figure Lengend Snippet: The phylogenetic relationship and protein sequence alignment of MAPKK proteins with MAPKK proteins. (A) Dendrogram of MAPKK proteins from Solanum tuberosum (St) (StMAPKK1–5), Arabidopsis thaliana (At) (AtMAPKK1–10), Solanum lycopersicum (Sl) (SlMAPKK1–5), and Oryza sativa (Os) (1,3,4,5,6,10–1 and 10–2); Bootstrap values were calculated from 500 resampled datasets with a 50% cut-off; MEGA-X software was used to establish the neighbor-joining phylogenetic tree. (B) Protein sequence of MAPKK proteins were aligned using DNAman software; Identical and similar amnio acids were marked in the same colors. The conserved subdomains were indicated on the bottom, using the Roman numbers (I-XI). The dark line marks the active site motif. Red asterisks indicate substrate specificity, and the black dots above the sequences represent activating sites. The conserved consensus motif GXGXXG is marked as I.

Article Snippet: DNAman tool (Lynnon Biosoft, San Ramon, CA, USA) was applied to multiple-sequence alignment of the conserved subdomains.

Techniques: Sequencing, Software