dna-microarray based transcriptome data Search Results


transcriptome affymetrix dna microarrays  (Thermo Fisher)
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Thermo Fisher transcriptome affymetrix dna microarrays
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dna-microarray based transcriptome data  (imaGenes GmbH)
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dna microarray technology  (Thermo Fisher)
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Confirmation of <t>microarray</t> results by sequantitative RT-PCR analysis . Total RNA was isolated from primary embryo fibroblasts (MEFs), ras V12 /E1A MEFs and ras V12 /E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.
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superscripttm ii rnase reverse transcriptase kit  (Thermo Fisher)
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Confirmation of <t>microarray</t> results by sequantitative RT-PCR analysis . Total RNA was isolated from primary embryo fibroblasts (MEFs), ras V12 /E1A MEFs and ras V12 /E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.
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agilent-based dna microarrays  (Agilent technologies)
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Confirmation of <t>microarray</t> results by sequantitative RT-PCR analysis . Total RNA was isolated from primary embryo fibroblasts (MEFs), ras V12 /E1A MEFs and ras V12 /E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.
Agilent Based Dna Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ht-12 gene chips  (Illumina Inc)
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Confirmation of <t>microarray</t> results by sequantitative RT-PCR analysis . Total RNA was isolated from primary embryo fibroblasts (MEFs), ras V12 /E1A MEFs and ras V12 /E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.
Ht 12 Gene Chips, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Confirmation of <t>microarray</t> results by sequantitative RT-PCR analysis . Total RNA was isolated from primary embryo fibroblasts (MEFs), ras V12 /E1A MEFs and ras V12 /E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.
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gene chip  (Thermo Fisher)
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Confirmation of <t>microarray</t> results by sequantitative RT-PCR analysis . Total RNA was isolated from primary embryo fibroblasts (MEFs), ras V12 /E1A MEFs and ras V12 /E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.
Gene Chip, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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streptavidin  (New England Biolabs)
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Confirmation of <t>microarray</t> results by sequantitative RT-PCR analysis . Total RNA was isolated from primary embryo fibroblasts (MEFs), ras V12 /E1A MEFs and ras V12 /E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.
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taq dna ligase  (New England Biolabs)
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Confirmation of <t>microarray</t> results by sequantitative RT-PCR analysis . Total RNA was isolated from primary embryo fibroblasts (MEFs), ras V12 /E1A MEFs and ras V12 /E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.
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Image Search Results


Confirmation of microarray results by sequantitative RT-PCR analysis . Total RNA was isolated from primary embryo fibroblasts (MEFs), ras V12 /E1A MEFs and ras V12 /E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.

Journal: Molecular Cancer

Article Title: Gene expression profiling of tumours derived from ras V12 /E1A-transformed mouse embryonic fibroblasts to identify genes required for tumour development

doi: 10.1186/1476-4598-4-4

Figure Lengend Snippet: Confirmation of microarray results by sequantitative RT-PCR analysis . Total RNA was isolated from primary embryo fibroblasts (MEFs), ras V12 /E1A MEFs and ras V12 /E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.

Article Snippet: In the present work, we used the Affymetrix-based DNA microarray technology to analyze gene expression profiles of tumour-derived from ras V12 /E1A-transformed primary mouse embryonic fibroblasts (MEFs), in order to identify genes associated with tumour development.

Techniques: Microarray, Reverse Transcription Polymerase Chain Reaction, Isolation, Binding Assay, Amplification