Journal: Frontiers in Microbiology

Article Title: Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia

doi: 10.3389/fmicb.2026.1748456

Figure Lengend Snippet: Sensitivity of the LAMP reaction for the detection of S. pneumoniae , S. aureus , H. influenzae , and M. pneumoniae. Visual LoD determined for the detection of S. pneumoniae (3.9 ×10 3 CFU/mL), S. aureus (1.7 ×10 5 CFU/mL), H. influenzae (8.2 ×10 3 CFU/mL), and M. pneumoniae (1.27 ×10 3 genome copies/reaction) (A) . Verification of amplification by 2% agarose gel electrophoresis (B) .

Article Snippet: Initially, tests included panel bacteria: S. pneumoniae ATCC 49619, S. aureus ATCC 25923, H. influenzae ATCC 49766, and purified DNA from M. pneumoniae (ATCC 29342DQ).

Techniques: Amplification, Agarose Gel Electrophoresis

Journal: Frontiers in Microbiology

Article Title: Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia

doi: 10.3389/fmicb.2026.1748456

Figure Lengend Snippet: Sensitivity of the designed primers for the detection of K. pneumoniae. Visual LoD (A) . The calculated LoD was 1.5 ×10 4 CFU/mL. Amplification was confirmed by 2% agarose gel electrophoresis (B) .

Article Snippet: Initially, tests included panel bacteria: S. pneumoniae ATCC 49619, S. aureus ATCC 25923, H. influenzae ATCC 49766, and purified DNA from M. pneumoniae (ATCC 29342DQ).

Techniques: Amplification, Agarose Gel Electrophoresis

Journal: Frontiers in Microbiology

Article Title: Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia

doi: 10.3389/fmicb.2026.1748456

Figure Lengend Snippet: Relationship between time to positivity and bacterial load for LAMP detection of S. pneumoniae , S. aureus , and H. influenzae . As the bacterial concentration decreased, the time required for detection increased across all three pathogens. For S. pneumoniae , high concentrations produced a clear positive result within 35 min. However, concentrations near the LoD (10 4 and 10 3 CFU/mL) required more than 55 min to be considered positive. Similarly, S. aureus showed a positive signal at 35 min when tested at high concentrations, whereas lower concentrations (around 10 4 CFU/mL) required over 45. In the case of H. influenzae , the highest concentration yielded a visible positive reaction at 25 min, while lower concentrations needed up to 45 min. Transition phase: In all cases, there was a stage where tubes began to show a greenish signal, indicating the onset of positivity, although the color had not yet fully developed to a strong green signal.

Article Snippet: Initially, tests included panel bacteria: S. pneumoniae ATCC 49619, S. aureus ATCC 25923, H. influenzae ATCC 49766, and purified DNA from M. pneumoniae (ATCC 29342DQ).

Techniques: Concentration Assay, Produced

Journal: medRxiv

Article Title: Direct detection and quantification of Mycobacterium tuberculosis from clinical samples by high-resolution melt qPCR

doi: 10.64898/2026.03.07.26347851

Figure Lengend Snippet: (A) Amplification plot of H37Rv replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.

Article Snippet: Using the M. tuberculosis H37Rv (ATCC #25618DQ) reference strain, we performed asymmetric PCR with Vent (exo-) DNA polymerase (New England Biolabs).

Techniques: Amplification, Control, Generated