Journal: The Scientific World Journal

Article Title: Peculiar Evolutionary History of miR390-Guided TAS3-Like Genes in Land Plants

doi: 10.1155/2013/924153

Figure Lengend Snippet: Analysis of PCR products in 1.5% agarose gel. Amplification of genomic DNA sequences flanked by miR390 and miR390* sites. PCR products were obtained on genomic DNAs with moss-specific primers [ , ]. Brachythecium rivulare (1), Nicotiana tabacum (control) (2), Marchantia polymorpha (3), Sphagnum squarrosum (4), Selaginella kraussiana (control) (5), Polytrichum commune (6), and primers with no template DNA (control) (7). (M), DNA size markers including bands ranging from 100 bp to 1000 bp with 100 bp step and 1500 bp band (Sibenzyme).

Article Snippet: For cloning, the PCR-amplified DNA bands isolated from gel were ligated into pGEM-T (Promega).

Techniques: Agarose Gel Electrophoresis, Amplification

Journal: Plants

Article Title: Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi

doi: 10.3390/plants12223821

Figure Lengend Snippet: ( A ) Structure of the GIP -silencing cassette. ( B ) Visualization of the synthesized cassette on agarose gel (M) DNA ladder of 100 bp; (1) the GIP -silencing cassette (551 pb).

Article Snippet: To estimate the concentration in comparison to the 500 bp band of the DNA ladder (Promega 100 bp ladder), the digested construct was visualized on a 1% agarose gel, excised with a sterile razor blade, and purified using the QIAquick ® Gel Extraction Kit following the manufacturer’s instructions (QIAGEN, Hilden, Germany).

Techniques: Synthesized, Agarose Gel Electrophoresis