Journal: Cell reports

Article Title: Peptide/Receptor Co-evolution Explains the Lipolytic Function of the Neuropeptide TLQP-21

doi: 10.1016/j.celrep.2019.07.101

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Article Snippet: Primestar , Takara/Fisher , Cat. No. R045A.

Techniques: Recombinant, Protease Inhibitor, Infection, Bicinchoninic Acid Protein Assay, cDNA Synthesis, SYBR Green Assay, Western Blot, shRNA, Plasmid Preparation, Control, Microarray, Labeling, Produced, Software, Drug discovery, Sequencing

Journal: Molecular Cell

Article Title: DNA Double-Strand Break Resection Occurs during Non-homologous End Joining in G1 but Is Distinct from Resection during Homologous Recombination

doi: 10.1016/j.molcel.2016.12.016

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Article Snippet: Rabbit-anti-DNA-PKcs , Novus Biologicals , NB100-658.

Techniques: Recombinant, Transfection, Plasmid Preparation, Purification, Western Blot, Sequencing, Software, Imaging, Microscopy, Laser-Scanning Microscopy

Journal: Human reproduction (Oxford, England)

Article Title: Defining the limits of detection for chromosome rearrangements in the preimplantation embryo using next generation sequencing.

doi: 10.1093/humrep/dey227

Figure Lengend Snippet: Figure 3 An example of how poor data quality can impact the detection of unbalanced chromosome segments for embryos from a 46,XX,t(2;9) (p24;q33) translocation carrier using next generation sequencing (NGS). The same SurePlex product is shown in both A and B. All quality control (QC) measures were above the acceptable level recommended by the manufacturer. (A) Shows the sample from a run with high data quality which resulted in the detection of the chromosome 2 duplication (2pter–2p25.1). (B) Shows the same sample from a run with suboptimal data quality (the number of reads passing filter were just above 250 000, which was well below the optimal 500 000 reads) which resulted in lack of detection of the chromosome 2 duplication.

Article Snippet: WGA was performed using the SurePlex DNA Amplification System as per the manufacturer’s instructions (Illumina).

Techniques: Translocation Assay, Next-Generation Sequencing, Control