Journal: STAR Protocols

Article Title: Genetic re-direction of canine primary T cells for clinical trial use in pet dogs with spontaneous cancer

doi: 10.1016/j.xpro.2021.100905

Figure Lengend Snippet:

Article Snippet: Sterile Filtered DMSO , GoldBio , Cat# D-361-10 CAS# 67-68-5.

Techniques: Functional Assay, Purification, Recombinant, Staining, Plasmid Preparation, Software, Modification, Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Biotin Transport-Targeting Polysaccharide-Modified PAMAM G3 Dendrimer as System Delivering α-Mangostin into Cancer Cells and C. elegans Worms

doi: 10.3390/ijms222312925

Figure Lengend Snippet: The 1 H-NMR spectra of α-mangostin ( A ), G3 2B12gh5M ( B ), and G3 2B10gh17M ( C ) conjugates in dmso-d 6 . The residual solvent peak at 2.5 and impurity resonances are marked with asterisks *. The PAMAM G3 core dendrimer resonances are labeled as CH 2 (G3) and NH(G3) in spectrum B and C. The resonances of α-mangostin, biotin, and glucoheptoamide are labeled by locants with M , B , and gh upper indexes, respectively, according to the atom numbering in .

Article Snippet: Trypsin-EDTA solution, hydrocortisone, 0.33% neutral red solution (3-amino-m-dimethylamino-2-methyl-phenazine hydrochloride), XTT sodium salt (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide inner salt), phenazinemethosulfate (PMS), crystal violet, 0.4% trypan blue solution, dimethylsulfoxide (dmso) for molecular biology, 5-Fluoro-2′-deoxy-uridine (FUdR), and other chemicals and buffers were purchased from Merck KGaA (Darmstadt, Germany).

Techniques: Labeling

Journal: International Journal of Molecular Sciences

Article Title: Biotin Transport-Targeting Polysaccharide-Modified PAMAM G3 Dendrimer as System Delivering α-Mangostin into Cancer Cells and C. elegans Worms

doi: 10.3390/ijms222312925

Figure Lengend Snippet: ( A ) The 1 H-NMR spectrum of G3 2B12gh vehicle in dmso-d 6 . The PAMAM G3 core dendrimer resonances are labeled as CH 2 (G3) and NH(G3). The resonances of biotin and D-glucoheptoamide residues are labeled with locants with B and gh . The residual solvent peak is labeled with an asterisk *. For atom numbering, see . ( B ) The selected chain of the PAMAM G3 dendrimer core with carbon atom numbering. Branch points at ternary nitrogen atoms are marked with wavy lines. R = H, biotin or gh residues.

Article Snippet: Trypsin-EDTA solution, hydrocortisone, 0.33% neutral red solution (3-amino-m-dimethylamino-2-methyl-phenazine hydrochloride), XTT sodium salt (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide inner salt), phenazinemethosulfate (PMS), crystal violet, 0.4% trypan blue solution, dimethylsulfoxide (dmso) for molecular biology, 5-Fluoro-2′-deoxy-uridine (FUdR), and other chemicals and buffers were purchased from Merck KGaA (Darmstadt, Germany).

Techniques: Labeling