Journal: International Journal of Molecular Sciences

Article Title: S mall M olecule Ar ranged T hermal P roximity C o a ggregation (smarTPCA)—A Novel Approach to Characterize Protein–Protein Interactions in Living Cells by Similar Isothermal Dose–Responses

doi: 10.3390/ijms23105605

Figure Lengend Snippet: Melting curve data of the CETSA experiments shown in Figure 1 (n: number of replicates). Thermal shifts induced by the inhibitors are reported as Δ T m to DMSO as the vehicle control.

Article Snippet: 40 μL of 10 mM stock solutions of the three different kinase inhibitors AMG-548 (Tocris, cat. no. 3920), SB203580 (Tocris, cat. no. 1202), or ERK 11e (VX-11e; Tocris, cat. no. 4465) in dimethyl sulfoxide (DMSO) were added separately to three of the aliquots to obtain a final concentration of 20 μM per compound.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: S mall M olecule Ar ranged T hermal P roximity C o a ggregation (smarTPCA)—A Novel Approach to Characterize Protein–Protein Interactions in Living Cells by Similar Isothermal Dose–Responses

doi: 10.3390/ijms23105605

Figure Lengend Snippet: ITDR-CETSA experiments for a compound concentration range between 0.3 nM and 20 µM as well as without compound (only DMSO) at a constant treatment temperature of 51 °C. ( a ) Immunoblots for one of the replicates of ITDR-CETSA experiments for living cells and cell extract, respectively. ( b ) Dose–response curves (solid lines) derived from integrated, normalized, and fitted signal intensities from the immunoblots. Datapoints and error bars represent means over the four replicates (n = 4) +/− one standard deviation. The fitted pEC50 values are summarized in . Data for ERK 11e are shown in .

Article Snippet: 40 μL of 10 mM stock solutions of the three different kinase inhibitors AMG-548 (Tocris, cat. no. 3920), SB203580 (Tocris, cat. no. 1202), or ERK 11e (VX-11e; Tocris, cat. no. 4465) in dimethyl sulfoxide (DMSO) were added separately to three of the aliquots to obtain a final concentration of 20 μM per compound.

Techniques: Concentration Assay, Western Blot, Derivative Assay, Standard Deviation

Journal: Cell Reports Medicine

Article Title: Unedited allogeneic iNKT cells show extended persistence in MHC-mismatched canine recipients

doi: 10.1016/j.xcrm.2023.101241

Figure Lengend Snippet:

Article Snippet: Sterile Filtered DMSO , GoldBio , Cat# D-361-10.

Techniques: Control, Virus, Recombinant, Sterility, Activation Assay, Expressing, Amplification, Clone Assay, Software, Modification, Saline, Cell Culture