digitalmicrograph Search Results


device ccd camera  (Gatan Inc)
97
Gatan Inc device ccd camera
Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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digitalmicrograph software 1.8  (Hrem Research Inc)
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Hrem Research Inc digitalmicrograph software 1.8
Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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digitalmicrograph  (CrystalMaker)
90
CrystalMaker digitalmicrograph
Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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smoire for digitalmicrograph  (Hrem Research Inc)
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Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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image processing package digitalmicrograph  (Hrem Research Inc)
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Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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smartalign (commercial) plug-ins for the digitalmicrograph software  (Hrem Research Inc)
90
Hrem Research Inc smartalign (commercial) plug-ins for the digitalmicrograph software
Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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qdpc plug-in for digitalmicrograph  (Basler)
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Basler qdpc plug-in for digitalmicrograph
Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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digitalmicrograph software  (Verlag GmbH)
90
Verlag GmbH digitalmicrograph software
Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
Digitalmicrograph Software, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stack acquisition tool digitalmicrograph script  (Digiscan GmbH)
90
Digiscan GmbH stack acquisition tool digitalmicrograph script
Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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ppa digitalmicrograph plug-in  (Hrem Research Inc)
90
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Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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digitalmicrograph (dm) script  (Hrem Research Inc)
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Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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commercial package hrem research msa plug-in for digitalmicrograph  (Hrem Research Inc)
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Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital <t>CCD</t> camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.
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Image Search Results


Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital CCD camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.

Journal:

Article Title: Replication of Herpes Simplex Virus 1 Depends on the ? 1 34.5 Functions That Facilitate Virus Response to Interferon and Egress in the Different Stages of Productive Infection

doi: 10.1128/JVI.78.14.7653-7666.2004

Figure Lengend Snippet: Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital CCD camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.

Article Snippet: Images were taken at various magnifications with a digital charge-coupled device (CCD) camera (Software digital micrograph; Gatan Inc.).

Techniques: Infection, Staining, Transmission Assay, Microscopy

Intracellular distribution of virions in cells infected with γ134.5 mutants with deletions in the amino-terminal domain. Confluent monolayers of MEF 3T6 cells in 35-mm dishes were infected with the indicated viruses at 0.5 PFU per cell. At 24 h postinfection, cells were harvested and processed for electron microscopic analysis as described in Materials and Methods. Digital images were taken at various magnifications with a Gatan digital CCD camera. R4002-infected MEF 3T6 cells (A), R931-infected MEF 3T6 cells (B), R908-infected MEF 3T6 cells (C), and R909-infected MEF 3T6 cells (D) are shown. Scale bars are shown in each picture. Nuc, nuclear region; Cyt, cytoplasm.

Journal:

Article Title: Replication of Herpes Simplex Virus 1 Depends on the ? 1 34.5 Functions That Facilitate Virus Response to Interferon and Egress in the Different Stages of Productive Infection

doi: 10.1128/JVI.78.14.7653-7666.2004

Figure Lengend Snippet: Intracellular distribution of virions in cells infected with γ134.5 mutants with deletions in the amino-terminal domain. Confluent monolayers of MEF 3T6 cells in 35-mm dishes were infected with the indicated viruses at 0.5 PFU per cell. At 24 h postinfection, cells were harvested and processed for electron microscopic analysis as described in Materials and Methods. Digital images were taken at various magnifications with a Gatan digital CCD camera. R4002-infected MEF 3T6 cells (A), R931-infected MEF 3T6 cells (B), R908-infected MEF 3T6 cells (C), and R909-infected MEF 3T6 cells (D) are shown. Scale bars are shown in each picture. Nuc, nuclear region; Cyt, cytoplasm.

Article Snippet: Images were taken at various magnifications with a digital charge-coupled device (CCD) camera (Software digital micrograph; Gatan Inc.).

Techniques: Infection