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Reagena Ltd
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Thermo Fisher
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Image Search Results
Journal: PLoS ONE
Article Title: NR4A2 Is Regulated by Gastrin and Influences Cellular Responses of Gastric Adenocarcinoma Cells
doi: 10.1371/journal.pone.0076234
Figure Lengend Snippet: A : Real-time cell migration monitored (0-24 h) in AGS-G R cells transfected with siNR4A2 or siCtr, with or without gastrin treatment (10 nM). Results show one representative of three biological replicas; mean ±SD of three technical replicas. B : Invasion assay with AGS-G R cells transfected with pCMX-NR4A2 or pCMX (control) was performed in 24-well plates containing 8-µm pore Matrigel-coated inserts (with or without 0.3 nM gastrin). Cells invading the lower surface of the membrane were stained with Reastain Quick-Diff reagents and total numbers of cells in 5 fields per membrane were counted. The mean of three independent experiments is shown.
Article Snippet: AGS-G R cells (4.0 x 10 4 cells/well) in 0.5 ml serum-free medium were plated in the insert with or without addition of gastrin (0.3 nM) for 24 h. Cells invading the lower surface of the membrane were stained with
Techniques: Migration, Transfection, Invasion Assay, Control, Membrane, Staining