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Image Search Results


The impact of CCl 4 and the bilberry fruit extract on pro-inflammatory mediators in liver homogenate a .

Journal: Antioxidants

Article Title: Anthocyanins Protect Hepatocytes against CCl 4 -Induced Acute Liver Injury in Rats by Inhibiting Pro-inflammatory mediators, Polyamine Catabolism, Lipocalin-2, and Excessive Proliferation of Kupffer Cells

doi: 10.3390/antiox8100451

Figure Lengend Snippet: The impact of CCl 4 and the bilberry fruit extract on pro-inflammatory mediators in liver homogenate a .

Article Snippet: The liver homogenate concentration level of Lipocalin-2 (Neutrophil gelatinase-associated lipocalin), CD68 (Cluster of Differentiation 68), TNF-α (tumor necrosis factor-alpha), IL-4 (interleukin-4), IL-6 (interleukin-6), and IL-13 (interleukin-13) were determined using a commercial ELISA kit (Rat NGAL ELISA kit, ab207925, Abcam, USA; Rat CD68 ELISA kit, CSB-E13297r, Cusabio, USA; Rat TNF-α ELISA kit, Antibodies-online, Germany; Rat IL-4 ELISA kit, Antibodies-online, Germany; Rat IL-6 ELISA kit, R&D Systems, USA; Rat IL-13 ELISA kit, Antibodies-online, Germany) according to the manufacturer’s instructions.

Techniques:

Effect of CCl 4 and bilberry fruit extract on histopathology of rat liver after 24 h (H&E and Immunohistochemical staining). I -group I (untreated group), II -group II (treated group), III -group III (CCl 4 ), IV -group IV (treated + CCl 4 ). ( A ) group I (untreated group), 100×; ( B ) group II (treated group), 100×; ( C1 – C11 ) group III (CCl 4 ); C1-Necrosis followed by minor and severe reversible change, 100×; C2-Necrosis followed by macrovesicular hepatocytes, 100×; C3-Necrosis followed by vacuolar hepatocytes, 100×; C4-Hemorrhagic coagulation necrosis, 100×; C5-Necrosis (🞲), macrovesicular hepatocytes (→), hydropic hepatocytes ( → ), inflammatory mononuclear infiltrate ( 🞲 ), 200×; C6-Hemorrhagic coagulation necrosis (🞲), 200×; C7-Necrosis (🞲), vacuolar hepatocytes (→), 200×; C8- macrovesicular fatty hepatocytes (→), 400×; C9-Necrosis (🞲), microvesicular fatty hepatocytes (→), 400×; C10-Necrosis (🞲), 400×; C11-Inflammatory mononuclear infiltrate (🞲), 400×; ( D1 – D3 ) group IV (treated + CCl 4 ); D1-minor reversible change (hydropic hepatocytes), 100×; D2- minor reversible change (vacuolar hepatocytes) and extended sinusoidal spaces, 200×; D3-Hydropic hepatocytes (→), 400×; ( E ) immunohistochemical detection of COX-2, 100×; ( F ) immunohistochemical detection of iNOS (NOS2), 100×; ( G ) immunohistochemical detection of TNF-α, 100×, ( H ) immunohistochemical detection of NGAL, 100×; ( I ) immunohistochemical detection Kupffer cells identification marker-CD68, 100×; ( J ) morphometric analysis extent of the necrotic area on a selected H&E field in the liver. The data show the average value ± S.D. (%) for 10 fields at the magnification of 200× for each animal in each group (H&E staining); ( K ) semiquantitative evaluation of COX-2, iNOS, TNF-α, NGAL, and CD68 immunohistochemistry staining. Staining intensity was graded as: - (negative), +/- (weak positive), + (positive), ++ (strongly positive), or +++ (very strongly positive).

Journal: Antioxidants

Article Title: Anthocyanins Protect Hepatocytes against CCl 4 -Induced Acute Liver Injury in Rats by Inhibiting Pro-inflammatory mediators, Polyamine Catabolism, Lipocalin-2, and Excessive Proliferation of Kupffer Cells

doi: 10.3390/antiox8100451

Figure Lengend Snippet: Effect of CCl 4 and bilberry fruit extract on histopathology of rat liver after 24 h (H&E and Immunohistochemical staining). I -group I (untreated group), II -group II (treated group), III -group III (CCl 4 ), IV -group IV (treated + CCl 4 ). ( A ) group I (untreated group), 100×; ( B ) group II (treated group), 100×; ( C1 – C11 ) group III (CCl 4 ); C1-Necrosis followed by minor and severe reversible change, 100×; C2-Necrosis followed by macrovesicular hepatocytes, 100×; C3-Necrosis followed by vacuolar hepatocytes, 100×; C4-Hemorrhagic coagulation necrosis, 100×; C5-Necrosis (🞲), macrovesicular hepatocytes (→), hydropic hepatocytes ( → ), inflammatory mononuclear infiltrate ( 🞲 ), 200×; C6-Hemorrhagic coagulation necrosis (🞲), 200×; C7-Necrosis (🞲), vacuolar hepatocytes (→), 200×; C8- macrovesicular fatty hepatocytes (→), 400×; C9-Necrosis (🞲), microvesicular fatty hepatocytes (→), 400×; C10-Necrosis (🞲), 400×; C11-Inflammatory mononuclear infiltrate (🞲), 400×; ( D1 – D3 ) group IV (treated + CCl 4 ); D1-minor reversible change (hydropic hepatocytes), 100×; D2- minor reversible change (vacuolar hepatocytes) and extended sinusoidal spaces, 200×; D3-Hydropic hepatocytes (→), 400×; ( E ) immunohistochemical detection of COX-2, 100×; ( F ) immunohistochemical detection of iNOS (NOS2), 100×; ( G ) immunohistochemical detection of TNF-α, 100×, ( H ) immunohistochemical detection of NGAL, 100×; ( I ) immunohistochemical detection Kupffer cells identification marker-CD68, 100×; ( J ) morphometric analysis extent of the necrotic area on a selected H&E field in the liver. The data show the average value ± S.D. (%) for 10 fields at the magnification of 200× for each animal in each group (H&E staining); ( K ) semiquantitative evaluation of COX-2, iNOS, TNF-α, NGAL, and CD68 immunohistochemistry staining. Staining intensity was graded as: - (negative), +/- (weak positive), + (positive), ++ (strongly positive), or +++ (very strongly positive).

Article Snippet: The liver homogenate concentration level of Lipocalin-2 (Neutrophil gelatinase-associated lipocalin), CD68 (Cluster of Differentiation 68), TNF-α (tumor necrosis factor-alpha), IL-4 (interleukin-4), IL-6 (interleukin-6), and IL-13 (interleukin-13) were determined using a commercial ELISA kit (Rat NGAL ELISA kit, ab207925, Abcam, USA; Rat CD68 ELISA kit, CSB-E13297r, Cusabio, USA; Rat TNF-α ELISA kit, Antibodies-online, Germany; Rat IL-4 ELISA kit, Antibodies-online, Germany; Rat IL-6 ELISA kit, R&D Systems, USA; Rat IL-13 ELISA kit, Antibodies-online, Germany) according to the manufacturer’s instructions.

Techniques: Histopathology, Immunohistochemical staining, Staining, Coagulation, Marker, Immunohistochemistry