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Image Search Results
Journal: International journal of molecular sciences
Article Title: Effect of Airborne Particulate Matter on the Immunologic Characteristics of Chronic Rhinosinusitis with Nasal Polyps.
doi: 10.3390/ijms23031018
Figure Lengend Snippet: Figure 5. The pivotal effect of PM10-induced IL-33 for Th2 differentiation effects of conditioned media on PM10-induced human nasal polyp derived fibroblast (NPDFs-CM) on the inflammatory cytokine production in CD4+ T cells. (A) Th2 differentiation was determined by the expression of GATA3 and ST2 (IL-33 receptor). (B,C) The protein levels of IFN-γ, IL-17A, IL-4, IL-13, and IL-10 in CD4+ T cell populations were determined by flow cytometry according to the addition of ST2. The graphic data represents the means ± SEM (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.0001 as compared to the PM10-treated group.
Article Snippet: TMCs (1 × 106 cells) were cultured with 1 μg/mL of phytohemagglutinin (PHA) in NPDF-CM for 72 h. For Th2 differentiation, CD4+ T cells from TMCs were purified using a CD4+ T Cell Isolation Kit MicroBeads (Miltenyi Biotech, Bisley, Surrey, UK), and purified CD4+ T cells were differentiated using a
Techniques: Derivative Assay, Expressing, Cytometry