Journal: International journal of molecular sciences

Article Title: Effect of Airborne Particulate Matter on the Immunologic Characteristics of Chronic Rhinosinusitis with Nasal Polyps.

doi: 10.3390/ijms23031018

Figure Lengend Snippet: Figure 5. The pivotal effect of PM10-induced IL-33 for Th2 differentiation effects of conditioned media on PM10-induced human nasal polyp derived fibroblast (NPDFs-CM) on the inflammatory cytokine production in CD4+ T cells. (A) Th2 differentiation was determined by the expression of GATA3 and ST2 (IL-33 receptor). (B,C) The protein levels of IFN-γ, IL-17A, IL-4, IL-13, and IL-10 in CD4+ T cell populations were determined by flow cytometry according to the addition of ST2. The graphic data represents the means ± SEM (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.0001 as compared to the PM10-treated group.

Article Snippet: TMCs (1 × 106 cells) were cultured with 1 μg/mL of phytohemagglutinin (PHA) in NPDF-CM for 72 h. For Th2 differentiation, CD4+ T cells from TMCs were purified using a CD4+ T Cell Isolation Kit MicroBeads (Miltenyi Biotech, Bisley, Surrey, UK), and purified CD4+ T cells were differentiated using a CellXVivo Human Th2 Cell Differentiation Kit (RnDSystems, Minneapolis, MN, USA) following the manufacturer’s instructions.

Techniques: Derivative Assay, Expressing, Cytometry

Journal: Hepatology Communications

Article Title: Hyperoxidized Albumin Modulates Platelets and Promotes Inflammation Through CD36 Receptor in Severe Alcoholic Hepatitis

doi: 10.1002/hep4.1440

Figure Lengend Snippet: Validation of platelet functionality in SAH. (A) Platelet activation/aggregation in SAH histogram shows the percent expression of PAC‐1, P‐selectin (CD62p), and intracellular Ca +2 (secretion) in SAH and HC. (B) Increased expression (relative change) of various mRNA in SAH platelets linked to platelet and complement activation. Protein expression of Gp2b/3a/β‐actin ratio in platelets of patients with SAH. Results expressed as mean ± SEM (* P < 0.05, ** P < 0.01 for SAH versus HC). (C) Patients with SAH showed higher plasma levels of PF‐4 (65.85 ± 0.9457 versus 81.13 ± 6.595 ng/mL, P < 0.02), P‐selectin (6.360 ± 0.1639 versus 10.118 ± 2.1492 ng/mL, P < 0.0001), and soluble cluster of differentiation 40 ligand (1,726 ± 134.4 versus 2,528 ± 1.525.4 pg/mL, P < 0.05). Circulating levels of PF‐4, P‐selectin, and sCD40L correlated with the MELD score in SAH (r > 0.5, P < 0.05). (D) Representative western blot of SNARE complex proteins in platelets isolated from SAH versus HC (n = 5). Results are expressed as mean ± SEM (* P < 0.05, ** P < 0.01, *** P < 0.001 as indicated).

Article Snippet: Plasma‐citrated blood samples were centrifuged for 15 minutes at 3,000 g , and levels of PF‐4 (E‐EL‐H2216; Elabscience, Houston, TX), P‐selectin (E‐EL‐H0917; Elabscience), and sCD40L (E‐EL‐H0035; Elabscience) were measured.

Techniques: Biomarker Discovery, Activation Assay, Expressing, Clinical Proteomics, Western Blot, Isolation