Journal: PLoS ONE

Article Title: Developmental Splicing Deregulation in Leukodystrophies Related to EIF2B Mutations

doi: 10.1371/journal.pone.0038264

Figure Lengend Snippet: List of the genes selected for QRT-PCR analysis.

Article Snippet: DIAPH3 , Diaphanous homolog 3 , Hs01107326_ml.

Techniques:

Journal: PLoS ONE

Article Title: Developmental Splicing Deregulation in Leukodystrophies Related to EIF2B Mutations

doi: 10.1371/journal.pone.0038264

Figure Lengend Snippet: Recapitulative data of microarray and QRT-PCR experiments.

Article Snippet: DIAPH3 , Diaphanous homolog 3 , Hs01107326_ml.

Techniques: Microarray

Journal: Cancer research

Article Title: A pro-tumorigenic mDia2-MIRO1 axis controls mitochondrial positioning and function in cancer-associated fibroblasts

doi: 10.1158/0008-5472.CAN-22-0162

Figure Lengend Snippet: A Quantification of total proteins secreted by sh-EV and -mDia2 HDFs. n=3.

Article Snippet: Proximity ligation assays (PLA) were performed with mDia2 (sc-293288, Santa Cruz, Santa Cruz, CA), MIRO1 (NBP1-59021, Novus Biologicals, Littleton, CA) and TRAK1 (H00022906-M01A, Thermo Fisher Scientific) antibodies (1:500) and MitoTracker Green as described for fluorescence microscopy, followed by ligation of probes specific for each antibody using a PLA Kit (DUO92101, Sigma-Aldrich).

Techniques:

Journal: Cancer research

Article Title: A pro-tumorigenic mDia2-MIRO1 axis controls mitochondrial positioning and function in cancer-associated fibroblasts

doi: 10.1158/0008-5472.CAN-22-0162

Figure Lengend Snippet: A Representative images of sh-EV and sh-mDia2 HDFs stained with MitoTracker (green), rhodamine-coupled phalloidin (red) and Hoechst (blue). Graph shows fluorescence intensity of MitoTracker relative to the highest intensity value. The relative distance is indicated with values from 1 to 10 (see example on the top). n=10.

Article Snippet: Proximity ligation assays (PLA) were performed with mDia2 (sc-293288, Santa Cruz, Santa Cruz, CA), MIRO1 (NBP1-59021, Novus Biologicals, Littleton, CA) and TRAK1 (H00022906-M01A, Thermo Fisher Scientific) antibodies (1:500) and MitoTracker Green as described for fluorescence microscopy, followed by ligation of probes specific for each antibody using a PLA Kit (DUO92101, Sigma-Aldrich).

Techniques: Staining, Fluorescence

Journal: Cancer research

Article Title: A pro-tumorigenic mDia2-MIRO1 axis controls mitochondrial positioning and function in cancer-associated fibroblasts

doi: 10.1158/0008-5472.CAN-22-0162

Figure Lengend Snippet: A Representative Western blot of total lysate of sh-EV or -mDia2 fibroblasts for mDia2, MIRO1, GAPDH, and α-tubulin.

Article Snippet: Proximity ligation assays (PLA) were performed with mDia2 (sc-293288, Santa Cruz, Santa Cruz, CA), MIRO1 (NBP1-59021, Novus Biologicals, Littleton, CA) and TRAK1 (H00022906-M01A, Thermo Fisher Scientific) antibodies (1:500) and MitoTracker Green as described for fluorescence microscopy, followed by ligation of probes specific for each antibody using a PLA Kit (DUO92101, Sigma-Aldrich).

Techniques: Western Blot

Journal: Cancer research

Article Title: A pro-tumorigenic mDia2-MIRO1 axis controls mitochondrial positioning and function in cancer-associated fibroblasts

doi: 10.1158/0008-5472.CAN-22-0162

Figure Lengend Snippet: A, B sh-EV or -mDia2 HDFs co-cultured for 7 days with SCC13 cells immunostained for E-cadherin (red) and counterstained with MitoTracker (green) and Hoechst (blue) (A). The mitochondrial distribution relative to the highest intensity value from the perinuclear region of fibroblasts to the interface with SCC13 cells was quantified (B). Relative distances range from 1-10. n=4-6.

Article Snippet: Proximity ligation assays (PLA) were performed with mDia2 (sc-293288, Santa Cruz, Santa Cruz, CA), MIRO1 (NBP1-59021, Novus Biologicals, Littleton, CA) and TRAK1 (H00022906-M01A, Thermo Fisher Scientific) antibodies (1:500) and MitoTracker Green as described for fluorescence microscopy, followed by ligation of probes specific for each antibody using a PLA Kit (DUO92101, Sigma-Aldrich).

Techniques: Cell Culture

Journal: Discover Oncology

Article Title: DIAPH3 is a multifaceted prognostic biomarker that links immunotherapy response to tumor microenvironment in prostate cancer

doi: 10.1007/s12672-026-04413-6

Figure Lengend Snippet: DIAPH3 expression and its prognostic value in TCGA cohort. A Pan-cancer analysis of DIAPH3 expression in TIMER2.0 database. B DIAPH3 expression in PCa and adjacent tissues of patients from GEPIA2 database, and normal prostate tissues were matched for GTEx database. C DIAPH3 expression in PCa and adjacent tissues from patients in Yinchuan cohort. D Association between DIAPH3 expression and BCRFS/PFS of TCGA PCa patients

Article Snippet: After blocking with 5% normal goat serum for 1 h, sections were incubated with primary antibody against DIAPH3 (rabbit polyclonal, 1:300 dilution; Proteintech, Cat#14342-1-AP) at 4 °C overnight.

Techniques: Expressing

Journal: Discover Oncology

Article Title: DIAPH3 is a multifaceted prognostic biomarker that links immunotherapy response to tumor microenvironment in prostate cancer

doi: 10.1007/s12672-026-04413-6

Figure Lengend Snippet: The association between DIAPH3 expression and prognosis of PCa patients with radical surgery in multiple datasets. A Association between DIAPH3 expression and BCRFS of PCa patients with radical surgery in Stockholm and Moffitt cohorts. B DIAPH3 expression in Yinchuan cohort patients with different progression status

Article Snippet: After blocking with 5% normal goat serum for 1 h, sections were incubated with primary antibody against DIAPH3 (rabbit polyclonal, 1:300 dilution; Proteintech, Cat#14342-1-AP) at 4 °C overnight.

Techniques: Expressing

Journal: Discover Oncology

Article Title: DIAPH3 is a multifaceted prognostic biomarker that links immunotherapy response to tumor microenvironment in prostate cancer

doi: 10.1007/s12672-026-04413-6

Figure Lengend Snippet: Development and validation of nomogram model predicting BCRFS and PFS for PCa after radical prostatectomy. A Nomogram model predicting BCRFS of PCa. B C-index of nomogram model and model without DIAPH3 for predicting BCRFS. C Nomogram model predicting PFS of PCa. D C-index of nomogram model and model without DIAPH3 for predicting PFS. Due to limitation of follow up time, the 5-year calibration curve in Yinchuan cohort was not calculated

Article Snippet: After blocking with 5% normal goat serum for 1 h, sections were incubated with primary antibody against DIAPH3 (rabbit polyclonal, 1:300 dilution; Proteintech, Cat#14342-1-AP) at 4 °C overnight.

Techniques: Biomarker Discovery

Journal: Discover Oncology

Article Title: DIAPH3 is a multifaceted prognostic biomarker that links immunotherapy response to tumor microenvironment in prostate cancer

doi: 10.1007/s12672-026-04413-6

Figure Lengend Snippet: The association between DIAPH3 expression and prognosis of PCa patients with radical radiotherapy. A Association between DIAPH3 expression and BCRFS of PCa patients with radical radiotherapy in UK cohort. B Association between DIAPH3 expression and PFS of PCa patients with radical radiotherapy in UK cohort. C BCRFS of PCa patients of integrated cohorts with radical radiotherapy and radical surgery in low DIAPH3 expression group. D BCRFS of PCa patients of integrated cohorts with radical radiotherapy and radical surgery in high DIAPH3 expression group. E BCRFS of PCa patients with radical radiotherapy and radical surgery in high DIAPH3 expression group after propensity score matching. F Subgroup analysis of BCRFS of PCa patients with radical radiotherapy and radical surgery in high DIAPH3 expression group after propensity score matching.(RP is the reference, Patients with high DIAPH3 results may benefit more from RRT)

Article Snippet: After blocking with 5% normal goat serum for 1 h, sections were incubated with primary antibody against DIAPH3 (rabbit polyclonal, 1:300 dilution; Proteintech, Cat#14342-1-AP) at 4 °C overnight.

Techniques: Expressing

Journal: Discover Oncology

Article Title: DIAPH3 is a multifaceted prognostic biomarker that links immunotherapy response to tumor microenvironment in prostate cancer

doi: 10.1007/s12672-026-04413-6

Figure Lengend Snippet: Exploration of potential biological process, somatic mutation and immune microenvironment status related to DIAPH3 expression. A Differential expressed genes between patients with low and high DIAPH3. B Gene function enrichment analysis for identified differential expressed genes based on KEGG annotation. C Somatic mutation and tumor immune microenvironment status between patients with low and high DIAPH3 expression

Article Snippet: After blocking with 5% normal goat serum for 1 h, sections were incubated with primary antibody against DIAPH3 (rabbit polyclonal, 1:300 dilution; Proteintech, Cat#14342-1-AP) at 4 °C overnight.

Techniques: Mutagenesis, Expressing

Journal: Discover Oncology

Article Title: DIAPH3 is a multifaceted prognostic biomarker that links immunotherapy response to tumor microenvironment in prostate cancer

doi: 10.1007/s12672-026-04413-6

Figure Lengend Snippet: The association between DIAPH3 expression and immunotherapy response in a pan-cancer cohort. A IPS in TCGA-PRAD patients with low and high DIAPH3 expression. B TIDE score in TCGA-PRAD patients with low and high DIAPH3 expression. C Association between DIAPH3 expression and overall survival of pan-cancer receiving immunotherapy, including bladder cancer, esophageal adenocarcinoma, glioblastoma, melanoma and urothelial cancers and so on. D Mediation analysis of immune cells in effect of DIAPH3 on immunotherapy reponse. *Note: The analysis and significant finding presented here are based on the integrated pan-cancer cohort

Article Snippet: After blocking with 5% normal goat serum for 1 h, sections were incubated with primary antibody against DIAPH3 (rabbit polyclonal, 1:300 dilution; Proteintech, Cat#14342-1-AP) at 4 °C overnight.

Techniques: Expressing

Journal: Discover Oncology

Article Title: DIAPH3 is a multifaceted prognostic biomarker that links immunotherapy response to tumor microenvironment in prostate cancer

doi: 10.1007/s12672-026-04413-6

Figure Lengend Snippet: Identification of potential drug interventions for DIAPH3 expression. A Exploratory analysis of DIAPH3 expression and inferred drug sensitivity based on GDSC database. The box plots illustrate the distribution of predicted IC50 values. These results represent computational predictions that require further statistical and experimental validation. B DIAPH3 expression and inferred drug sensitivity based on CTRP and PRISM databases

Article Snippet: After blocking with 5% normal goat serum for 1 h, sections were incubated with primary antibody against DIAPH3 (rabbit polyclonal, 1:300 dilution; Proteintech, Cat#14342-1-AP) at 4 °C overnight.

Techniques: Expressing, Biomarker Discovery

Journal: Tumor Biology

Article Title: Role of MEK1 and DIAPH3 expression in colorectal adenoma-carcinoma sequence

doi: 10.3233/tub-230038

Figure Lengend Snippet: Fig. 1. MEK1 and DIAPH3 expression in colorectal carcinoma and adenoma. (A) Marked MEK1 cytoplasmic staining in a case of grade II CRC (B) Moderate MEK1 cytoplasmic staining in moderately dysplastic epithelium of adenoma. (C) Moderate DIAPH3 cytoplasmic staining in in a case of grade II CRC. (D) Negative DIAPH3 staining in moderately dysplastic adenoma. (x 200).

Article Snippet: Sections were immunostained with 0.1 ml of anti- DIAPH3 Antibody (Novus Biologicals cat # NBP1-89099) and anti- MEK1 [p Ser221] Antibody (Novus Biologicals cat # NBP1-41823).

Techniques: Expressing, Staining

Journal: PLoS ONE

Article Title: Transcription factor programming of human ES cells generates functional neurons expressing both upper and deep layer cortical markers

doi: 10.1371/journal.pone.0204688

Figure Lengend Snippet: List of primers/probes used in single cell qPCR analysis.

Article Snippet: DIAPH3 , , cortical-spinal/motor neurons , Hs01107330_m1.

Techniques: Expressing, Control

Journal: BMC Gastroenterology

Article Title: Novel stromal biomarker screening in pancreatic cancer patients using the in vitro cancer-stromal interaction model

doi: 10.1186/s12876-020-01556-w

Figure Lengend Snippet: Survival analysis using the TCGA RNA-Seq dataset

Article Snippet: The rabbit polyclonal DIAPH3 antibody (GTX102892; Gene Tex, Irvine, CA, USA) was used at a dilution of 1:200.

Techniques:

Journal: BMC Gastroenterology

Article Title: Novel stromal biomarker screening in pancreatic cancer patients using the in vitro cancer-stromal interaction model

doi: 10.1186/s12876-020-01556-w

Figure Lengend Snippet: RNA validation analysis using the qRT-PCR. The mRNA expression levels of BRIP1, DIAPH3, MCM8, WDHD1, and GAPDH were evaluated by qRT-PCR in the PDAC and non-PDAC tissues of five resected specimens. Overall fold change was calculated as the median and interquartile-range (IQR): a BRIP1 (IQR, 0.07 to 0.11), b DIAPH3 (IQR, 0.15 to 1.35), c MCM8 (IQR, 0.03 to 0.3), d WDHD1 (IQR, 0.02 to 0.12). qRT-PCR, Real-Time Quantitative Reverse Transcription PCR; PDAC, pancreatic ductal adenocarcinoma

Article Snippet: The rabbit polyclonal DIAPH3 antibody (GTX102892; Gene Tex, Irvine, CA, USA) was used at a dilution of 1:200.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Reverse Transcription

Journal: BMC Gastroenterology

Article Title: Novel stromal biomarker screening in pancreatic cancer patients using the in vitro cancer-stromal interaction model

doi: 10.1186/s12876-020-01556-w

Figure Lengend Snippet: DIAPH3 expression in PDAC fibroblasts. The stromal DIAPH3 expression pattern is depicted in a – b . Weak ( a , b ) and strong ( c , d ) expression of DIAPH3 was shown by cytoplasmic staining of PDAC fibroblasts. Strong staining of DIAPH3 in stromal fibroblasts was defined as readily visible staining. Comparable IHC staining was observed in lymphocytes, and intensity of stained stromal fibroblasts were judged by reference to those of lymphocytes. Strong staining of DIAPH3 was also defined as the staining in at least 30% of stromal fibroblasts. DIAPH3, Diaphanous-related formin-3; PDAC, pancreatic ductal adenocarcinoma

Article Snippet: The rabbit polyclonal DIAPH3 antibody (GTX102892; Gene Tex, Irvine, CA, USA) was used at a dilution of 1:200.

Techniques: Expressing, Staining, Immunohistochemistry

Journal: BMC Gastroenterology

Article Title: Novel stromal biomarker screening in pancreatic cancer patients using the in vitro cancer-stromal interaction model

doi: 10.1186/s12876-020-01556-w

Figure Lengend Snippet: Stromal DIAPH3 expression levels for overall survival analysis. Kaplan-Meier curves showing OS for 216 PDAC patients in TMAs according to stromal DIAPH3 expression levels. Stromal DIAPH3 expression was a negative predictive marker for OS in PDAC patients who underwent surgery. OS, overall survival; PDAC, pancreatic ductal adenocarcinoma; TMA, tissue microarray; DIAPH3, diaphanous-related formin-3

Article Snippet: The rabbit polyclonal DIAPH3 antibody (GTX102892; Gene Tex, Irvine, CA, USA) was used at a dilution of 1:200.

Techniques: Expressing, Marker, Microarray